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Pulmonary Cell News| ESC & iPSC News 12.04 February 1, 2017 | |
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TOP STORYInterspecies Organogenesis Generates Autologous Functional Islets Researchers injected mouse pluripotent stem cells (PSCs) into Pdx-1-deficient rat blastocysts, they generated rat-sized pancreata composed of mouse-PSC-derived cells. Islets subsequently prepared from these mouse–rat chimeric pancreata were transplanted into mice with streptozotocin-induced diabetes. The transplanted islets successfully normalized and maintained host blood glucose levels for over 370 days in the absence of immunosuppression. [Nature] Abstract | Press Release | |
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PUBLICATIONS(Ranked by impact factor of the journal)Interspecies Chimerism with Mammalian Pluripotent Stem Cells Scientists established a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and showed enrichment of rat pluripotent stem cell-derivatives in several tissues of gene-edited organogenesis-disabled mice. [Cell] Abstract | Press Release | Graphical Abstract Investigators showed that neural progenitor cells derived from human induced pluripotent stem cells (iPSCs) retain the parental mitochondrial DNA (mtDNA) profile and exhibit a metabolic switch toward oxidative phosphorylation. [Cell Stem Cell] Abstract | Press Release | Graphical Abstract Researchers reported the derivation of human-induced pluripotent stem cells (hiPSCs) from patients with diagnosed age-related macular degeneration (AMD), including two donors with the rare ARMS2/HTRA1 homozygous genotype. The hiPSC-derived retinal pigment epithelium cells produced several AMD/drusen-related proteins, and those from the AMD donors show significantly increased complement and inflammatory factors, which are most exaggerated in the ARMS2/HTRA1 lines. [Cell Stem Cell] Abstract | Graphical Abstract The authors described a platform based on a genetically diverse panel of induced pluripotent stem cells (iPSCs) that reproduces susceptibility to develop a cardiotoxic drug response. They generated iPSC-derived cardiomyocytes from patients presenting in vivo with extremely low or high changes in cardiac repolarization in response to a pharmacological challenge with sotalol. In vitro, the responses to sotalol were highly variable but strongly correlated to the inter-individual differences observed in vivo. [Elife] Full Article Researchers generated PCSK1 (PC1/3)-deficient human embryonic stem cell (hESC) lines using both short hairpin RNA and CRISPR-Cas9, and investigated pro-opiomelanocortin (POMC) processing using hESC-differentiated hypothalamic neurons. The increased levels of unprocessed POMC and the decreased ratios of processed POMC-derived peptides in both PCSK1 knockdown and knockout hESC-derived neurons phenocopied POMC processing reported in PC1/3-null mice and PC1/3-deficient patients. [Stem Cell Reports] Full Article Differentiation of Human Induced Pluripotent Stem Cells to Mammary-Like Organoids Investigators developed a reliable two-step protocol to generate human mammary-like organoids from induced pluripotent stem cells (iPSCs). Non-neural ectoderm-cell-containing spheres, referred to as mEBs, were first differentiated and enriched from iPSCs using MammoCult medium. They then generated mammary-like organoids from 10-day mEBs using 3D floating mixed gel culture and a three-stage differentiation procedure. [Stem Cell Reports] Full Article The authors described the highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. The simple addition of laminin fragments to a cell suspension during passaging accelerated the adhesion of single dissociated hPSCs onto culture vessels that were not precoated with any culture substrate. [Sci Rep] Full Article Scientists investigated the effect of human TNNI3K on the differentiation of mouse embryonic stem cells into cardiomyocytes. They found that the number of troponin-T positive cells was greater in the group with TNNI3K overexpression as compared to that in control group, while less such cells were detected in the mTnni3k knock-down group as evaluated on flow cytometry and ImageXpress Micro system. [Cell Physiol Biochem] Full Article | |
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REVIEWSInduced Pluripotent Stem Cell Research in the Era of Precision Medicine The authors discuss how induced pluripotent stem cell research will further contribute to human health in the coming era of precision medicine. [Stem Cells] Abstract Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field. | |
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INDUSTRY NEWSCIRM Approves New Funding to UC San Diego Researchers Fighting Zika Virus and Cancer The Independent Citizens Oversight Committee of the California Institute for Regenerative Medicine (CIRM) has approved a pair of $2 million awards to University of California San Diego School of Medicine researchers to advance studies of new treatments for Zika virus infections and the use of stem cell-derived natural killer cells to target ovarian cancer and other malignancies. [University of California] Press Release PPMD Awards $250,000 Grant to UT Southwestern Medical Center to Explore CRISPR/Cas9 Technology PPMD announced a $250,000 grant to be awarded to Dr. Eric Olson and the Department of Molecular Biology at UT Southwestern Medical Center. This grant, part of PPMD’s Gene Transfer Initiative, will support Dr. Olson’s ongoing study of CRISPR/Cas9 technology as a potential treatment for Duchenne. [Parent Project Muscular Dystrophy (PPMD)] Press Release | |
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POLICY NEWSOn 22 April, Empiricists Around the Country Will March for Science The grassroots team coordinating the March for Science on Washington, D.C., have now set a date: 22 April. And they are inviting organizers in cities around the world to lead parallel demonstrations. [ScienceInsider] Editorial Trump Agenda Threatens US Legacy of Science Diplomacy A newly minted leader with no experience governing at home or establishing policy abroad now oversees the United States’ vast diplomatic enterprise. US President Donald Trump has a deep bench of scientific and technical expertise to tap across multiple government agencies — but it is not clear that he will use it. [Nature News] Editorial
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EVENTSNEW Epigenetic Regulation of Stem Cell Differentiation Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Associate Principle Scientist – In Vitro Biologist (AstraZeneca) NEW Head Scientist – iPSC Research (Victor Chang Cardiac Research Institute) Postdoctoral Position – Stem Cells and Epigenetics (University of Michigan) Postdoctoral Fellow – Multiple Areas (University of Oklahoma) Regulatory Support Scientist – Biological Product Development (Cook MyoSite, Inc.) Postdoctoral Fellow – Development of the Cardiovascular System (Goethe University Frankfurt) Research Scientist – Stem Cell Gene Editing (Universal Cells Inc.) Assistant or Associate Member – Stem CellGene Therapy (Fred Hutchinson Cancer Research Center) Postdoctoral Fellow – Pluripotent Stem Cell Technology (University of California, Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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Home ESC & iPSC News Volume 12.04 | Feb 1 2017
