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Pulmonary Cell News| ESC & iPSC News 14.02 January 23, 2019 | |
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TOP STORYIdentifying cis Elements for Spatiotemporal Control of Mammalian DNA Replication Researchers created a series of CRISPR-mediated deletions and inversions of a pluripotency-associated topologically associating domain (TAD) in mouse ESCs. CTCF-associated domain boundaries were dispensable for replication timing (RT). CTCF protein depletion weakened most TAD boundaries but had no effect on RT or A/B compartmentalization genome-wide. [Cell] Abstract | Press Release | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells Introduction of an MHC class I-restricted T cell receptor (TCR) in PSCs produced naive, antigen-specific CD8αβ+ T cells that lacked endogenous TCR expression and showed anti-tumor efficacy in vitro and in vivo. Functional assays and RNA sequencing aligned PSC-derived T cells with primary naive CD8+ T cells. [Cell Stem Cell] Abstract | Press Release | Graphical Abstract Functional validation of clinical-grade iPSC-retinal pigment epithelium (RPE) patches revealed specific features that distinguished transplantable from nontransplantable patches. Compared to RPE cells in suspension, the biodegradable scaffold approach improved integration and functionality of RPE patches in rats and in a porcine laser-induced RPE injury model that mimicked age-related macular degeneration-like eye conditions. [Sci Transl Med] Abstract | Press Release | Editorial Albeit with no observable cytotoxicity, significant differentiation retardation was found in female mouse ESCs (mESCs) upon low-dose silver nanoparticle (AgNP) exposure. Mechanistic investigations uncovered expedited inactivation for the inactive X chromosome and attenuated maintenance of the active X chromosome state during mESC differentiation upon the challenge of low-dose AgNPs, indicative of disordered X chromosome inactivation. [ACS Nano] Abstract | Graphical Abstract Generation of Lung Organoids from Human Pluripotent Stem Cells In Vitro Human lung organoids could be generated from human PSCs (hPSCs) in 50-85 days, and bud tip progenitor organoids could be generated in 22 days. The two hPSC-derived models were benchmarked with human fetal tissue and found to be representative of human fetal-like tissue. [Nat Protoc] Abstract Investigators found that the naive-primed pluripotency axis was preserved across species, although the timing of the transition to a primed state was species specific. They also identified markers for distinguishing human naive and primed pluripotency as well as strong co-regulatory relationships between lineage markers and epigenetic regulators that were exclusive to naive cells. [Cell Rep] Full Article | Press Release | Graphical Abstract Acquisition of Dynamic Function in Human Stem Cell-Derived β Cells Scientists report a differentiation strategy focused on modulating transforming growth factor β signaling, controlling cellular cluster size, and using an enriched serum-free media to generate stem cell-derived β cells that expressed β cell markers and underwent glucose-stimulated insulin secretion with first- and second-phase dynamic insulin secretion. [Stem Cell Reports] Full Article | Press Release | Graphical Abstract Researchers applied the method for obtaining isogenic cells homozygous for variant B cystatin C, a recessive risk factor for age-related macular degeneration and Alzheimer’s disease, in both iPSCs and a human RPE cell line. Bi-allelic gene edited clones were validated by sequencing, demonstrating that the double antibiotic templates approach worked efficiently for both iPSCs and human differentiated cells. [Sci Rep] Full Article The authors showed that a novel chondrogenic differentiation method of iPSCs by using the combination of 3-D cultivation in UPAL gel and multi-step differentiation via mesenchymal stem cell-like cells could efficiently and specifically differentiate iPSCs into chondrocytes. [J Biomed Mater Res A] Abstract A Human Embryonic Stem Cell-Based Model for Benzo[a]pyrene-Induced Embryotoxicity Investigators used a human ESC derived embryoid body (EB) as an in vitro model to investigate the embryotoxicity effects of benzo[a]pyrene (B[a]P). EBs were exposed to B[a]P for 14 days, and the morphology, viability and differentiation of the cells were analyzed, in addition to the molecular changes. [Reprod Toxicol] Abstract | Graphical Abstract Subscribe to one of our other 19 science newsletters such as Cell Therapy News & Mesenchymal Cell News. | |
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REVIEWSInduced Pluripotent Stem Cells for Neural Drug Discovery Recent advances with iPSCs have enabled modeling of human diseases with patient-derived neural cells. Utilizing iPSC-derived neurons advances compound screening and evaluation of drug efficacy. Therefore, application of iPSC-derived human neurons is a new direction for neuronal drug discovery. [Drug Discov Today] Full Article Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field. | |
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INDUSTRY NEWSSTEMCELL Technologies has released mTeSRâ„¢ Plus, an enhanced version of mTeSRâ„¢1, the most widely published feeder-free human PSC maintenance medium. The new medium has undergone successful beta testing with several members of the Stem Cell COREdinates group and has completed rigorous in-house validation testing. [STEMCELL Technologies Inc.] Press Release The Center for Beta Cell Therapy in Diabetes and ViaCyte, Inc. announced that a human stem cell-derived product candidate has been implanted in type 1 diabetes patients at a subtherapeutic dose. [ViaCyte, Inc.] Press Release The Cystinosis Research Foundation announced that the FDA granted approval for the first human clinical trial for stem cell and gene therapy treatment for cystinosis. Previous studies with mice found that the treatment rescued the kidneys, eyes and thyroid from the effects of cystine, effectively preventing the symptoms of cystinosis. [The Cystinosis Research Foundation (Business Wire, Inc.)] Press Release Thyas Co. Ltd. signed a licensing agreement with iPS Academia Japan, Inc. Under the agreement, Thyas obtains exclusive rights on patents related to production and use of iPS cell-derived T cells, and non-exclusive rights on the patents related to iPS cell generation, for development and commercialization of autologous immunotherapy of cancers and infectious diseases. [Thyas Co. Ltd. (GlobeNewswire, Inc)] Press Release | |
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POLICY NEWSCRISPR-Baby Scientist Fired by University The decision by the Southern University of Science and Technology in Shenzhen follows a report of findings from an investigation into He’s work by provincial health authorities. A probe by the Guangdong health ministry found that He broke national regulations against using gene-editing for reproductive purposes, Chinese state media agency Xinhua reported. [Nature News] Editorial Number of EU Research Students in Britain Drops From Pre-Referendum High If the trend persists, and there is preliminary evidence from the Russell Group — a body that represents 24 of the United Kingdom’s leading research universities — that it will, this would mark a reversal of years of growth, from around 12,700 postgraduate research students in 2008-2009 to more than 15,000 in 2015-2016. [Nature News] Editorial Congress to Vote on Previous Spending Deals That Could Presage Final 2019 Budgets Congress plans to pass 2019 spending bills that would give healthy increases to the National Science Foundation, NASA, and a handful of other science agencies that are now closed because of the partial shutdown of the US government. [ScienceInsider] Editorial Scientific Societies Worry Plan S Will Make Them Shutter Journals, Slash Services An existential threat. That’s what scientific societies supported by journal subscriptions call Plan S. Introduced in September 2018 by European research funders and endorsed by others since then, the plan will require that grantees’ papers be immediately available free of charge. [ScienceInsider] Editorial
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EVENTSNEW Regenerative Medicine Workshop Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Position – Stem Cells & Neurodegeneration (Lund University) Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.) Scientist – Cell Culture Media and Cell Line Development (STEMCELL Technologies Inc.) Postdoctoral Fellow – Stem Cell Biology (City of Hope) Senior Laboratory Research Scientist – Pluripotent Stem Cell Research (The Francis Crick Institute) Postdoctoral Fellow – Genetics and Epigenomics of iPSC Biology (Stanford University) Assistant Associate Professor/Professor in Residence – Stem Cell Program (UC Davis Medical Center) Postdoctoral Fellows – Human iPSC Cell Differentiation Research (Boston Children’s Hospital) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (UC Davis) Postdoctoral Position – Stem Cells and Diabetes (A*STAR) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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