ESC & iPSC News 14.46 November 27, 2019 | |
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TOP STORYResearchers investigated the fidelity of cytidine deaminase-mediated base editing in human iPSCs by whole genome sequencing after sustained or transient base editor expression. [Nat Commun] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)Automated Design of Pluripotent Stem Cell Self-Organization The authors combined genetic engineering with computational modeling, machine learning, and mathematical pattern optimization to create a data-driven approach to control human PSC self-organization by knock down of genes previously shown to affect stem cell colony organization, CDH1 and ROCK1. [Cell Syst] Abstract | Graphical Abstract A Versatile ES Cell-Based Melanoma Mouse Modeling pPlatform Scientists described the development and validation of an ESC-genetically engineered mouse model platform for rapid modeling of melanoma in mice. The platform incorporated twelve clinically relevant genotypes composed of combinations of four driver alleles and regulatory alleles to spatiotemporally controlled the perturbation of genes-of-interest. [Cancer Res] Abstract Researchers used eSpCas9 to successfully achieve seamless correction of the two most prevalent Usher syndrome (USH)2A mutations in iPSCs of patients with USH or autosomal recessive retinitis pigmentosa. [Mol Ther Methods Clin Dev] Abstract | Full Article The authors developed a protocol based on HBA promoter-driven transient expression of transcription factors by means of adeno-associated viral vectors, that allowed them to generate very consistent numbers of dopaminergic neurons from four different human iPSC lines. [Cell Death Dis] Full Article Investigators found that SOX21 was required for rostral forebrain fate specification. SOX21 knockout led to activation of Wnt signaling, resulting in caudalization of regional identity of rostral forebrain neural progenitor cells. [Stem Cell Reports] Full Article Scientists showed that Hesx1 was expressed in the preimplantation mouse embryo, where it was required during developmental diapause. Absence of Hesx1 led to reduced expression of epiblast and primitive endoderm determinants and failure of diapaused embryos to resume embryonic development after implantation. [Stem Cell Reports] Full Article The authors used a human (h)iPSC cardiomyocyte (CM) model of dilated cardiomyopathy containing phospholamban (PLB) mutation R14del, where they observed that adeno-associated virus 2-driven expression of PLB mutants rescued arrhythmic calcium transients and alleviated decreased calcium transport. [J Mol Cell Cardiol] Abstract Investigators established a disease model of hedgehog-related tumorigenesis with Gorlin syndrome-derived induced pluripotent stem cells (GS-iPSCs). Induced neural progenitor cells (NPCs) from GS-iPSCs showed constitutive high GLI1 expression and higher sensitivity to SMO inhibition compared with wild-type induced NPCs. [Lab Invest] Abstract Quantification of the Morphological Characteristics of hESC Colonies Using image analysis and computational tools, scientists precisely quantified properties using phase-contrast images of human (h)ESC colonies of different sizes during days two, three and four after plating. [Sci Rep] Full Article Exosome/Microvesicle Content Is Altered in LRRK2 Mutant iPSC-Derived Neural Cells Researchers profiled two microvesicles from different Parkinson’s disease tissue sources: neural progenitor cells derived from an endogenous adult stem/progenitor cell, called adult human neural progenitor cells; and Leucine-Rich Kinase 2 (LRRK2) gene identified patient iPSCs. [J Comp Neurol] Abstract Subscribe to one of our other 19 science newsletters such as Cell Therapy News & Mesenchymal Cell News. | |
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REVIEWSAdvances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications The authors provide a comprehensive portrait of important progress in stem cell research and development. Innovative technologies and real-world applications are emphasized. [Stem Cell Rev Rep] Abstract An Insight into Reprogramming Barriers to iPSC Generation Scientists present a comprehensive overview of the barriers that inhibit reprogramming and the understanding of which will pave the way to develop safe strategies for efficient reprogramming. [Stem Cell Rev Rep] Abstract Visit our reviews page to see a complete list of reviews in the ESC & iPSC research field. | |
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INDUSTRY NEWSNovoheart is pleased to announce a collaboration with global biopharmaceutical company AstraZeneca, in an effort to develop the world’s first human-specific in vitro, functional model of heart failure with preserved ejection fraction, a common condition especially among the elderly and in women. [Novoheart Holdings, Inc.] Press Release Speeding Cell, Gene Therapy Development An innovative new major private-public partnership led by Harvard and MIT aims to accelerate one of the hottest and most promising areas of medical research: cell and gene therapy. [Harvard University] Press Release | |
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POLICY NEWSFive Ways China Must Cultivate Research Integrity A swift increase in scientific productivity has outstripped the country’s ability to promote rigor and curb academic misconduct; it is time to seize solutions. [Nature News] Editorial Elsevier Signs First Open-Access Deal in the United States Publishing giant Elsevier has signed its first open-access deal with a US institution, Carnegie Mellon University (CMU) in Pittsburgh, Pennsylvania, Inside Higher Ed reports. The arrangement, which CMU announced on 21 November, will allow CMU scholars to publish articles in any Elsevier journal on an immediately free-to-read basis. [ScienceInsider] Editorial
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EVENTSNEW Advances in Stem Cells and Regenerative Medicine Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Fellow – iPSC Heart Cells (Stanford University) Research Techician – iPSC Culture Lab (University of Luxembourg) Postdoctoral Associate – Stem Cell Research (University of Minnesota Twin Cities) Faculty Position – Stem Cell Research (Yale University School of Medicine) Multiple Positions – Stem Cell Biology (NIH NCATS Stem Cell Translation Laboratory) Research Assistant – Stem Cells and Engineering (New York Blood Center) Postdoctoral Researcher – CRISPR/Cas9 in ESCs (Institute of Molecular Biology GmbH) Postdoctoral Researcher – iPSC Models in Neurology (IRIBHM) Postdoctoral Scientist – Regenerative Medicine (Cedars-Sinai Medical Center) PhD Position – Human Inducible Stem Cells (University of Fribourg) Research Associate – Computational Image Analysis and AI (University of Bristol) Assistant Specialist – Division of Cardiovascular Medicine (University of California, Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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