ESC & iPSC News Volume 15.10 | Mar 18 2020

TOP STORY

Extracellular Nanovesicles for Packaging of CRISPR-Cas9 Protein and sgRNA to Induce Therapeutic Exon Skipping

Scientists developed an extracellular nanovesicle-based ribonucleoprotein delivery system named NanoMEDIC by utilizing two distinct homing mechanisms. NanoMEDIC achieved over 90% exon skipping efficiencies in skeletal muscle cells derived from Duchenne muscular dystrophy patient iPSCs. [Nat Commun] Full Article

(Ranked by impact factor of the journal)

Phosphoproteomics Identifies a Bimodal EPHA2 Receptor Switch that Promotes Embryonic Stem Cell Differentiation

Researchers showed that EPHA2 maintained pluripotency and restrained commitment by antagonising ERK1/2 signaling. Upon ESC differentiation, FGF4 utilised a bimodal strategy to disable EPHA2, which was accompanied by transcriptional induction of EFN ligands. [Nat Commun] Full Article

Specialized Cytonemes Induce Self-Organization of Stem Cells

Investigators describe specialized ESC-generated cytonemes that reacted to trophoblast stem cell-secreted Wnts. Cytoneme formation and length were controlled by actin, intracellular calcium stores, and components of the Wnt pathway. [Proc Natl Acad Sci USA] Full Article

Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation

Scientists showed that alkaline phosphatase placental-like 2 (ALPPL2) could interact with the RNA-binding protein IGF2BP1 to stabilize the mRNA levels of the naive pluripotency transcription factors TFCP2L1 and STAT3 to regulate naive pluripotency. [Cell Rep] Full Article | Graphical Abstract

A Susceptibility Locus on Chromosome 13 Profoundly Impacts the Stability of Genomic Imprinting in Mouse Pluripotent Stem Cells

Researchers documented a genetic determinant that segregated mouse pluripotent cells into stable and unstable cell lines. Unstable lines exhibited hypermethylation at Dlk1-Dio3 and other imprinted loci, in addition to impaired developmental potential. [Cell Rep] Full Article | Graphical Abstract

Enhanced Inner Ear Organoid Formation from Mouse Embryonic Stem Cells by Photobiomodulation

Scientists determined the optimal condition to differentiate the ESCs into otic organoid using different culture techniques and photobiomodulation parameters. [Mol Ther Methods Clin Dev] Abstract | Full Article | Graphical Abstract

A Novel Model of Liver Cancer Stem Cells Developed from Induced Pluripotent Stem Cells

The authors generated a new model of liver cancer stem cells by converting mouse iPSCs with hepatocellular carcinoma cell line Huh7 cells conditioned medium. [Br J Cancer] Full Article

Syngeneic Mesenchymal Stem Cells Reduce Immune Rejection after Induced Pluripotent Stem Cell-Derived Allogeneic Cardiomyocyte Transplantation

To determine whether mesenchymal stem cell (MSC) co-transplantation could reduce immune rejection after allogeneic iPSC-derived cardiomyocyte transplantation, the latter cell type, harboring a luciferase transgene, was subcutaneously transplanted alone or together with syngeneic MSCs into BALB/c mice. [Sci Rep] Full Article

The Transcription Factor-MicroRNA Regulatory Network during hESC-Chondrogenesis

Scientists developed an unbiased approach for integrating transcriptome and miRome using protein-protein interactions, transcription factor regulation and miRNA target interactions and identified key regulatory networks prominent in human ESC chondrogenesis. [Sci Rep] Full Article

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Application of Adult and Pluripotent Stem Cells in Interstitial Cystitis/Bladder Pain Syndrome Therapy: Methods and Perspectives

The authors provide a brief overview of the etiology, pathophysiology, diagnosis, and treatment of interstitial cystitis/bladder pain syndrome as well as a summary of adult stem cells and PSCs [J Clin Med] Abstract | Full Article Download

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Coriell Institute for Medical Research Awarded $8.6 Million Biobanking Contract from National Institute on Aging

The National Institute on Aging has extended its biobanking contract with the Coriell Institute for Medical Research for an additional five years. [Coriell Institute for Medical Research] Press Release

Journals, Peer Reviewers Cope with Surge in COVID-19 Publications

Academic journals have already taken steps to accelerate the flow of peer-reviewed information by expediting editorial and peer review for coronavirus-related manuscripts and granting them open-access status upon publication. [The Scientist] Editorial

Near-Lockdown in the Bay Area ‘Ratchets Up’ Challenges for Local Biotechs and Labs

Seven counties in the San Francisco Bay Area went into near-lockdown — ushering in a new reality for biopharma companies and biomedical labs. The “shelter in place” order offers significant leeway for medical research. [STAT News] Editorial

NEW Cambridge International Stem Cell Symposium 2020
September 27-29, 2020
Cambridge, United Kingdom

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Position – Pluripotent Stem Cell-Derived Human Lung Organoids (Columbia University Medical Center)

NEW Technician – Stem Cell Culture (University of Luxembourg)

NEW Postdoctoral Position – Pluripotency (Cancer Research Center)

NEW Laboratory Research Scientist – Human Embryo and Stem Cell Unit (The Francis Crick Institute)

Research Specialist – Stem Cells for Regenerative Medicine (University of Illinois at Chicago)

Postdoctoral Position – Human Pluripotent Stem Cells to Hepatobiliary Tissues (Johns Hopkins University School of Medicine)

Head – Automated Stem Cell and Organoid Facility (Fondazione Human Technopole)

Postdoctoral Associate – Cerebral Organoids (Weill Cornell Medicine)

Postdoctoral Fellowship – Stem Cell Niche Biology (University of California, San Francisco)

Assistant Specialist – Division of Cardiovascular Medicine (University of California, Davis)

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