ESC & iPSC News Volume 16.31 | Aug 11 2021

    By
    Justin.choi
    -
    0
    115







    2021-08-11 | ESC 16.31


    ESC & iPSC News by STEMCELL Technologies
    Vol. 16.31 – 11 August, 2021
    TOP STORY

    Dissecting OCT4 Defines the Role of Nucleosome Binding in Pluripotency

    Researchers systematically dissected OCT4 to show that nucleosome binding was encoded within the DNA-binding domain and yet could be uncoupled from free-DNA binding. They found that stable interactions between OCT4 and nucleosomes were continuously required for maintaining the accessibility of pluripotency enhancers in stem cells.
    [Nature Cell Biology]

    Abstract

    Virtual Conference Exhibition: Organoids
    PUBLICATIONSRanked by the impact factor of the journal

    Induction and Expansion of Human PRRX1+ Limb-Bud-Like Mesenchymal Cells from Pluripotent Stem Cells

    Scientists reported a protocol that leveraged PRRX1–tdTomato reporter human PSCs for the selective induction of expandable and ontogenetically defined PRRX1+ limb-bud-like mesenchymal cells under defined xeno-free conditions.
    [Nature Biomedical Engineering]

    Abstract

    Human Sensorimotor Organoids Derived from Healthy and Amyotrophic Lateral Sclerosis Stem Cells Form Neuromuscular Junctions

    Investigators generated sensorimotor organoids containing physiologically functional neuromuscular junctions and applied the model to different subgroups of amyotrophic lateral sclerosis.
    [Nature Communications]

    Full Article

    CTCF Is a Barrier for 2C-Like Reprogramming

    The authors showed that the CCCTC-binding factor (CTCF) was a barrier for 2C-like reprogramming. Depletion of CTCF in ESCs efficiently promoted spontaneous and asynchronous conversion to a 2C-like state and was reversible upon restoration of CTCF levels.
    [Nature Communications]

    Full Article

    Generation of Insulin-Producing Pancreatic β Cells from Multiple Human Stem Cell Lines

    Scientists detailed a six-stage planar differentiation methodology for generating human pluripotent stem cell–derived pancreatic β cells that secreted high amounts of insulin in response to glucose stimulation.
    [Nature Protocols]

    Abstract

    Identification of Cancer-Related Mutations in Human Pluripotent Stem Cells Using RNA-Seq Analysis

    Researchers discussed the relevance of detecting mutations for various human PSCs (hPSCs) applications, and presented a detailed protocol to identify cancer-related point mutations using data from RNA sequencing, an assay commonly performed during the growth and differentiation of hPSCs.
    [Nature Protocols]

    Abstract

    A Novel Approach for Studying Mast Cell-Driven Disorders: Mast Cells Derived from Induced Pluripotent Stem Cells

    Investigators developed a novel strategy for the rapid and efficient differentiation of mast cells from human iPSCs.
    [Journal of Allergy and Clinical Immunology]

    Abstract

    Establishment of an Integration-Free Human Induced Pluripotent Stem Cell Line (TJCi001-a) from Normal Bone Marrow-Derived Mesenchymal Stem Cells

    Scientists generated an iPSC line from bone marrow-mesenchymal stem cells employing a non-integrating episomal vector. The generated iPSCs expressed pluripotency markers, showed a normal karyotype, and exhibited the potential for in vitro differentiation into three germ layers.
    [Stem Cell Research]

    Full Article

    Generation of Bi-Allelic MYBPC3 Truncating Mutant and Isogenic Control from an iPSC Line of a Patient with Hypertrophic Cardiomyopathy

    The authors reprogrammed skin fibroblasts from a hypertrophic cardiomyopathy patient carrying a heterozygous MYBPC3 truncating mutation into human iPSC (hiPSC) and used CRISPR/Cas9 to generate bi-allelic MYBPC3 truncating mutation and isogenic control hiPSC lines.
    [Stem Cell Research]

    Full Article

    A Comprehensive Analysis of Gene Expression Changes in a High Replicate and Open-Source Dataset of Differentiating hiPSC-Derived Cardiomyocytes

    Researchers performed a comprehensive analysis of the transcriptional changes occurring during human iPSC (hiPSC) differentiation to cardiomyocytes. Using single cell RNA-seq, they sequenced > 20,000 single cells from 55 independent samples representing two differentiation protocols and multiple hiPSC lines.
    [Scientific Reports]

    Full Article

    Return to your lab safely and efficiently with these guides and resources so that you can focus more on moving your research goals forward.
    REVIEWS

    iPSC-Derived Natural Killer Cells for Cancer Immunotherapy

    Investigators detail the benefits of using iPSCs in natural killer cell immunotherapies and discuss the challenges that must be overcome before this approach becomes mainstream in the clinic.
    [Molecules and Cells]

    Abstract

    INDUSTRY AND POLICY NEWS

    Scientist Develops Human-Based Models to Study Neurodevelopmental Disorders

    Yogita Adlakha, an Inspire Faculty Fellow, has developed human-based models to study neuron development and neurodevelopmental disorders such as autism, which can help design treatment strategies for such brain disorders.
    [Outlook India]

    Press Release

    FEATURED EVENT

    ISSCR: Stem Cells – From Basic Science to Clinical Translation

    October 27 – 29, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Research Assistant – Pharmacology

    The Scripps Research Institute – La Jolla, California, United States

    PhD Student – Stem Cell Biology in Intestinal Tumorigenesis

    Philipps University of Marburg – Marburg, Germany

    Postdoctoral Associate – Neuroimmunology of Brain Repair

    MSH Medical School Hamburg – Hamburg, Germany

    Assistant Professor – Translational Metabolism

    Dalhousie University – Halifax, Nova Scotia, Canada

    Postdoctoral Positions – Stem Cell-Based Therapy

    UCLA Jules Stein Eye Institute – Los Angeles, California, United States

    > See All Jobs

    Submit an article, publication, job or event
    Brought to you by
    stemcell-logo-for newsletter-2
    ESC & iPSC News Twitter