ESC & iPSC News Volume 16.35 | Sep 8 2021

2021-09-08 | ESC 16.35

ESC & iPSC News by STEMCELL Technologies
Vol. 16.35 – 8 September, 2021

Developmental Chromatin Programs Determine Oncogenic Competence in Melanoma

Using a combination of a human PSC–derived cancer model along with zebrafish transgenesis, researchers demonstrated that the transforming ability of BRAFV600E along with additional mutations depended on the intrinsic transcriptional program present in the cell of origin.

AbstractGraphical AbstractPress

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In Vitro Reconstitution of the Whole Male Germ-Cell Development from Mouse Pluripotent Stem Cells

Investigators showed an in vitro reconstitution of whole male germ-cell development by PSCs. Mouse ESCs were induced into primordial germ cell-like cells, which were expanded for epigenetic reprogramming.
[Cell Stem Cell]

AbstractGraphical Abstract

A Genome-Engineered Bioartificial Implant for Autoregulated Anticytokine Drug Delivery

The authors used CRISPR-Cas9 genome editing of iPSCs to create a synthetic gene circuit that sensed changing levels of endogenous inflammatory cytokines to trigger a proportional therapeutic response.
[Science Advances]

Full Article

Single-Cell Transcriptome of Early Hematopoiesis Guides Arterial Endothelial-Enhanced Functional T Cell Generation from Human PSCs

Scientists performed extensive single-cell transcriptomic analyses to map fate choices and gene expression patterns during hematopoietic differentiation of human PSCs and showed that oxidative metabolism was dysregulated during in vitro directed differentiation.
[Science Advances]

Full Article

OCT4 Cooperates with Distinct ATP-Dependent Chromatin Remodelers in Naïve and Primed Pluripotent States in Human

Researchers mapped the OCT4 interactomes in naïve and primed human ESCs (hESCs), revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes. In naïve hESCs, OCT4 was associated with both BRG1 and BRM, the two paralog ATPases of the BAF complex.
[Nature Communications]

Full Article

Improved Modeling of Human AD with an Automated Culturing Platform for iPSC Neurons, Astrocytes and Microglia

Investigators generated a induced pluripotent stem cell Alzheimer’s disease model using human derived cells, which showed signs of Aβ plaques, dystrophic neurites around plaques, synapse loss, dendrite retraction, axon fragmentation, phospho-Tau induction, and neuronal cell death in one model.
[Nature Communications]

Full Article

A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium

Scientists established a stepwise method to differentiate iPSCs into retinal pigment epithelium (RPE) (iPSC-RPE), which enabled efficient isolation and purification of patient-derived iPSC-RPE cells with high quality.
[Methods in Molecular Biology]


Patient-Specific iPSCs Carrying an SFTPC Mutation Reveal the Intrinsic Alveolar Epithelial Dysfunction at the Inception of Interstitial Lung Disease

Researchers presented a human in vitro model permitting investigation of epithelial-intrinsic events culminating in AEC2 dysfunction, using patient-specific iPSCs carrying an AEC2-exclusive disease-associated variant (SFTPCI73T).
[Cell Reports]

Full ArticleGraphical Abstract

Generation of Human Blastocyst-Like Structures from Pluripotent Stem Cells

The authors developed a 3D, two-step induction protocol for generating blastocyst-like structures from human extended PSCs.
[Cell Discovery]

Full Article

The Aryl Hydrocarbon Receptor Promotes Differentiation during Mouse Preimplantational Embryo Development

Scientists showed that the aryl hydrocarbon receptor (AhR) regulated pluripotency factors and maintained the metabolic activity required for proper embryo differentiation. AhR-lacking embryos showed a pluripotent phenotype characterized by a delayed expression of trophectoderm differentiation markers.
[Stem Cell Reports]

Full Article

Cell Cycle-Coupled Changes in the Level of Reactive Oxygen Species Support the Proliferation of Human Pluripotent Stem Cells

Using human ESCs as well as human iPSCs, investigators demonstrated that the level of reactive oxygen species in pluripotent cells oscillated in accordance with the cell cycle progression with the peak occurring at transition from S to G2/M phase of the cycle.
[Stem Cells]

AbstractFull Article

Identification of New Transcription Factors that Can Promote Pluripotent Reprogramming

The chromatin accessibility and transcriptional level of human ESCs and human urine cells were compared by Assay for Transposase-Accessible Chromatin with high-throughput sequencing and RNA sequencing RNA-seq to identify potential reprogramming factors.
[Stem Cell Reviews and Reports]

Full ArticleGraphical Abstract

Fluorescent Tagging of Endogenous Heme Oxygenase-1 in Human Induced Pluripotent Stem Cells for High Content Imaging of Oxidative Stress in Various Differentiated Lineages

Researchers presented the generation and application of a fluorescent human iPSC reporter line for heme oxygenase-1, which was considered a sensitive and reliable biomarker for the oxidative stress response.
[Archives of Toxicology]

Full Article

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Restoring Normal Islet Mass and Function in Type 1 Diabetes through Regenerative Medicine and Tissue Engineering

The authors discuss the potential regenerative capacity of the remaining islet cells and the utility of stem cell-derived β-like cells to restore β-cell function and describe tissue engineering approaches that might improve the engraftment, function, and survival of β-cell replacement therapies.
[Lancet Diabetes & Endocrinology]

Full Article

Pluripotent Stem Cells for Skeletal Tissue Engineering

Investigators review the use of human PSCs for skeletal tissue engineering, the different methods for characterization of skeletal cells and constructs at the tissue and single-cell level, and indicate newer resources not yet fully utilized in this field.
[Critical Reviews in Biotechnology]

Full Article


Adaptimmune Enters into a Strategic Collaboration with Genentech to Research, Develop, and Commercialize Cancer-Targeted Allogeneic T-Cell Therapies

Adaptimmune Therapeutics plc announced that it has entered into a strategic collaboration and license agreement with Genentech to develop and commercialize allogeneic cell therapies to treat multiple oncology indications. Adaptimmune will be responsible for developing clinical candidates using its iPSC derived allogeneic platform to produce T-cells.
[Adaptimmune Therapeutics plc]

Press Release


The NYSCF Conference – New York Stem Cell Foundation

October 19 – 20, 2021

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Biomedical Engineering – Biomaterials, Organoids, and Organ-on-a-Chip

University of Glasgow – Glasgow, Scotland, United Kingdom

Postdoctoral Fellow – Pathogenesis of Cancer and Neurodegenerative Diseases

St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

Postdoctoral Scientist – Organoid Research

Helmholtz Zentrum Münche – Neuherberg, Germany

Postdoctoral Research Fellow – Cardiac and Endothelial Cell Function

Stanford University – La Jolla, California, United States

Development Engineer – Cell Therapies and Regenerative Medicine

Danaher – Toronto, Ontario, Canada

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