 | | Vol. 16.39 – 6 October, 2021 |
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| The authors examined the molecular mechanisms coordinating the naïve to primed transition by using inducible ESC to genetically eliminate all RAS proteins. They showed that differentiated RASKO ESCs remained trapped in an intermediate state of pluripotency with naïve-associated features.
[Science Advances] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| Single-cell RNA sequencing across multiple stages of an in vitro primordial germ cell-like cells (PGCLC) differentiation system showed that PGCLC genes initially expressed in the naïve pluripotent stage became homogeneously dismantled in germline competent epiblast like-cells.
[Nature Communications] |
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| Scientists reported the establishment of a human congenital cataract in vitro model through differentiation of patient-specific iPSCs into regenerated lenses.
[NPJ Regenerative Medicine] |
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| Investigators reported the development of a novel human iPSC-derived, inducible neuronal model of Niemann-Pick disease, type C1, and demonstrated the utility of this new cell line in high-throughput drug/chemical screens to identify potential therapeutic agents.
[BMC Biology] |
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| Scientists analyzed loss of imprinting (LOI) in a large cohort of both bulk and single-cell RNA sequencing samples of naive and primed human PSCs (hPSCs). They showed that naive hPSCs exhibited high levels of non-random LOI, with bias toward paternally methylated imprinting control regions.
[Stem Cell Reports] |
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| Researchers reported the first-ever application of doggybone DNA (dbDNA™) vectors to generate human iPSCs. They achieved comparable iPSC reprogramming efficiencies using transiently expressing dbDNA™ vectors with the same iPSC reprogramming coding sequences as the state-of-the-art OriP/EBNA1 episomal vectors.
[Molecular Therapy-Methods & Clinical Development] |
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| Investigators showed that the transcripts of Gadd45 family genes, including Gadd45a, Gadd45b, and Gadd45g, were gradually increased upon mouse ESC differentiation. Upregulation of Gadd45 members decreased cell proliferation and induced endodermal and trophectodermal lineages.
[Cell Death Discovery] |
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| To explore the relationship between PLCγ2 and TREM2 and the role of PLCγ2 in regulating immune cell function, the authors generated human iPSC- derived macrophages from isogenic lines with homozygous PLCG2 knockout.
[Scientific Reports] |
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| Researchers used miR-27b-inducible human iPSCs (hiPSCs) to investigate the roles of miR-27b in the undifferentiated and early-differentiated stages. In undifferentiated hiPSCs, miR-27b suppressed the expression of pluripotency markers and cell proliferation.
[Scientific Reports] |
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| Scientists used Resf1 knockout cell lines to determine the requirements of RESF1 for ESC self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells
[Life Science Alliance] |
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| Investigators presented a novel vitrification-based method for adherent human iPSCs, designed for automated handling by microfluidic approaches and with ready-to-use potential, in suspension-based bioreactors after thawing, for example.
[Cryobiology] |
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| The past decade has seen considerable advances in in vitro gametogenesis, which aims to recreate germ cell development from PSCs in culture.
[Science] |
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| Human PSCs hold great promise for generating endodermal cells and their derivatives for the development of new therapeutics against many life-threatening diseases. Scientists review recent advances in metabolic and epigenetic regulation of endoderm differentiation.
[Trends in Cell Biology] |
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| The authors focus on computational approaches for the analysis of single-cell omics and imaging data and discuss their use to address many of the major challenges involved in the development, validation, and use of cells obtained from pluripotent cell differentiation.
[Cell Reports Methods] |
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| The University of California, Riverside, has received a grant of $5 million from the California Institute for Regenerative Medicine, the state’s stem cell agency, to train young scientists and physicians in stem cell research.
[University of California, Riverside] |
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| November 1 – 3, 2021
Virtual |
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| | Buck Institute for Research on Aging – Novato, California, United States |
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| | Imperial College London – London, England, United Kingdom |
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| | The International Society for Cell & Gene Therapy – Vancouver, British Columbia, Canada |
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| | University of Copenhagen – Copenhagen, Denmark |
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| | Imperial College London – London, England, United Kingdom |
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