ESC & iPSC News Volume 16.44 | Nov 10 2021

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    2021-11-10 | ESC 16.44


    ESC & iPSC News by STEMCELL Technologies
    Vol. 16.44 – 10 November, 2021
    TOP STORY

    High-Impact FN1 Mutation Decreases Chondrogenic Potential and Affects Cartilage Deposition via Decreased Binding to Collagen Type II

    Researchers aimed to identify the causal pathogenic mutation in an early-onset osteoarthritis family, followed by functional studies in human iPSCs in an in vitro organoid cartilage model.
    [Science Advances]

    Full Article


    A Labroots 2021 Webinar: iPSCs As Models, Part 1: What Are Induced Pluripotent Stem Cells and How Do You Use Them?, presented by Dr. Anjana Nityanandam, Director of the Human Stem Cell Lab core facility at St. Jude Children’s Research Hospital. Click to Watch Now.
    PUBLICATIONSRanked by the impact factor of the journal

    Inability to Switch from ARID1A-BAF to ARID1B-BAF Impairs Exit from Pluripotency and Commitment towards Neural Crest Formation in ARID1B-Related Neurodevelopmental Disorders

    The authors showed that ARID1B-Brg1/Brm associated factor (BAF) regulated the exit from pluripotency and lineage commitment by attenuating thousands of enhancers and genes of the NANOG and SOX2 networks. In iPSCs, these enhancers were maintained active by ARID1A-containing BAF.
    [Nature Communications]

    Full Article

    p53 Inactivation Unmasks Histone Methylation-Independent WDR5 Functions That Drive Self-Renewal and Differentiation of Pluripotent Stem Cells

    Wdr5 deletion in p53-null mouse ESCs led to impaired self-renewal, defective retinal neuroectoderm differentiation, and de-repression of germ cell/meiosis-specific genes.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    PCSK9 Regulates the NODAL Signaling Pathway and Cellular Proliferation in hiPSCs

    Proprotein convertase subtilisin kexin type 9 (PCSK9) inhibition in human iPSCs (hiPSCs) with the use of short hairpin RNA, CRISPR/cas9-mediated knockout, or endogenous PCSK9 loss-of-function mutation R104C/V114A unveiled its new role as a potential cell cycle regulator through the NODAL signaling pathway.
    [Stem Cell Reports]

    Full Article

    Cell-Cell Communication through FGF4 Generates and Maintains Robust Proportions of Differentiated Cell Types in Embryonic Stem Cells

    Scientists reported that the differentiation of robust proportions of epiblast-like and primitive endoderm-like cells in mouse ESC cultures emerged at the population level through cell-cell communication via a short-range fibroblast growth factor 4 (FGF4) signal.
    [Development]

    Full Article

    Thin Films of Functionalized Carbon Nanotubes Support Long-Term Maintenance and Cardio-Neuronal Differentiation of Canine Induced Pluripotent Stem Cells

    Investigators proposed thin films of functionalized carbon nanotubes as alternatives for the feeder-free in vitro culture of canine iPSCs, considered as the cellular model.
    [Nanomedicine-Nanotechnology Biology and Medicine]

    AbstractGraphical Abstract

    Developmental Defects and Impaired Network Excitability in a Cerebral Organoid Model of KCNJ11 p.V59M-Related Neonatal Diabetes

    Researchers generated cerebral organoids derived from human iPSCs (hiPSCs) possessing the KCNJ11 mutation p.Val59Met (V59M) and from non-pathogenic/normal hiPSCs.
    [Scientific Reports]

    Full Article

    Use of Induced Pluripotent Stem Cells to Build Isogenic Systems and Investigate Type 1 Diabetes

    The authors differentiated iPSC from one donor into dendritic cells, macrophages, endothelial cells, and β-cells, and engineered T cell avatars from the same donor to provide an in vitro platform to study genetic influences on critical cellular interactions.
    [Frontiers in Endocrinology]

    Full Article

    Mutated JAK2 Signal Transduction in Human Induced Pluripotent Stem Cell (iPSC)-Derived Megakaryocytes

    iPSCs were virally transduced to express wild-type JAK2, JAK2p.V617F, or JAK2p.N542_E543del, differentiated into megakaryocytes in the presence versus absence of doxycycline, and compared to ensure that the differences were solely from mutated JAK2 expressions.
    [Platelets]

    Abstract

    Neuronal and Cardiac Toxicity of Pharmacological Compounds Identified through Transcriptomic Analysis of Human Pluripotent Stem Cell-Derived Embryoid Bodies

    Scientists used human iPSC-derived embryoid bodies as an in vitro model to investigate the embryotoxic effects of a carefully selected set of pharmacological compounds.
    [Toxicology and Applied Pharmacology]

    Abstract


    Andreas Kurtz spearheaded a global stem cell registry to make hPSC data more accessible. Watch the webinar.
    REVIEWS

    Mechanical Regulation of Early Vertebrate Embryogenesis

    The authors review insights into mechanical control of early vertebrate development, including the role of forces in tissue patterning and embryonic axis formation.
    [Nature Reviews Molecular Cell Biology]

    Abstract

    INDUSTRY AND POLICY NEWS

    EdiGene Enters Research Collaboration with the University of Wisconsin-Madison on In Vivo RNA Editing Therapies

    EdiGene, Inc. has entered into a research collaboration with the University of Wisconsin–Madison as part of the company’s effort of translating proprietary LEAPER™ RNA editing technology into in vivo therapies.
    [EdiGene, Inc.]

    Press Release

    Dendreon Pharmaceuticals and Shoreline Biosciences Announce CMC and Manufacturing Alliance to Advance the Future of iPSC Cellular Therapy

    Dendreon Pharmaceuticals and Shoreline Biosciences, a biotechnology company developing allogeneic off-the-shelf, standardized, and targeted natural killer and macrophage cellular immunotherapies derived from iPSC for cancer, announced an alliance to advance the future of iPSC-derived cellular therapies.
    [Shoreline Biosciences]

    Press Release

    FEATURED EVENT

    Stem Cell Society Singapore Symposium 2021

    December 8 – 9, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Automation Scientist – Modeling Pediatric Diseases

    St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

    Researcher – Organoid Models for Drug Discovery

    KU Leuven – Leuven, Brussels

    Postdoctoral Research Fellow – Mammalian Synthetic Biology and Organoid Engineering

    The University of British Columbia – Vancouver, British Columbia, Canada

    Postdoctoral Fellow – Organoid Development

    Roche – Basel, Switzerland

    Postdoctoral Scholar – Regenerative Medicine

    University of Southern California School of Dentistry – Los Angeles, California, United States

    > See All Jobs

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