ESC & iPSC News Volume 17.08 | Mar 2 2022

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    2022-03-02 | ESC 17.08


    ESC & iPSC News by STEMCELL Technologies
    Vol. 17.08 – 2 March, 2022
    TOP STORY

    8C-Like Cells Capture the Human Zygotic Genome Activation Program In Vitro

    The authors reported the discovery of human 8-cell (8C)-like cells among naive ESCs, which transcriptionally resembled the 8C human embryo.
    [Cell Stem Cell]

    Full ArticleGraphical Abstract

    Quantify Cytokines with Confidence with High-Performance ELISA Kits
    PUBLICATIONSRanked by the impact factor of the journal

    Ultraefficient Extracellular Vesicle-Guided Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes

    Researchers demonstrated the use of extracellular vesicles derived from ESCs undergoing cardiac differentiation as biomimetic tools to induce cardiac reprogramming with extremely high efficiency.
    [Science Advances]

    Full Article

    Glutamine-Dependent Signaling Controls Pluripotent Stem Cell Fate

    Scientists revealed that, although all three germ lineages were capable of de novo glutamine (Gln) synthesis, only ectoderm generated sufficient Gln to sustain cell viability and differentiation, and this finding clarified lineage fate restrictions under Gln withdrawal.
    [Developmental Cell]

    Full ArticleGraphical Abstract

    Epigenetic Inheritance Is Gated by Naïve Pluripotency and Dppa2

    Using genome-wide CRISPR screening, the authors identified molecular factors that restricted heritable memory of epialleles in naïve PSCs, and demonstrated that removal of chromatin factor Dppa2 unlocked the potential for epigenetic inheritance uncoupled from DNA sequence.
    [EMBO Journal]

    Full ArticleGraphical Abstract

    BEND3 Safeguards Pluripotency by Repressing Differentiation-Associated Genes

    Investigators found that BEND3 was highly expressed in PSCs, and the induction of differentiation resulted in the down-regulation of BEND3.
    [Proceedings of the National Academy of Sciences of the United States of America]

    Abstract

    An HNF1α Truncation Associated with Maturity-Onset Diabetes of the Young Impairs Pancreatic Progenitor Differentiation by Antagonizing HNF1β Function

    Scientists used maturity-onset diabetes of the young 3 (MODY3) patient and CRISPR/Cas9-engineered hiPSCs grown as 3D organoids to investigate how HNF1αp291fsinsC affected hiPSC differentiation during pancreatic development.
    [Cell Reports]

    Full Article

    dCas9 Fusion to Computer-Designed PRC2 Inhibitor Reveals Functional TATA Box in Distal Promoter Region

    The authors identified critical polycomb repressive complex 2 (PRC2) functional sites at high resolution. They fused a computationally designed protein, EED binder, which competed with EZH2 and thereby inhibited PRC2 function, to dCas9 to allow for PRC2 inhibition at a precise locus using gRNA.
    [Cell Reports]

    Full ArticleGraphical Abstract

    RNA-Seq Is Not Required to Determine Stable Reference Genes for qPCR Normalization

    Investigators demonstrated that the statistical approach to determine the best reference genes from commonly used conventional candidates was more important than the preselection of ‘stable’ candidates from RNA-Seq data.
    [PLoS Computational Biology]

    Full Article

    Tumor RNA Transfected DCs Derived from iPS Cells Elicit Cytotoxicity against Cancer Cells Induced from Colorectal Cancer Patients In Vitro

    Researchers investigated iPSC-dendritic cells–in vitro transcriptional RNA (ivtRNA) which were induced by transfecting ivtRNA obtained from tumors of three colorectal cancer patients, and examined its antitumor effect.
    [Scientific Reports]

    Full Article

    Metabolites of Soil Microorganisms Modulate Amyloid β Production in Alzheimer’s Neurons

    Scientists conducted a library screening to quantify amyloid β and neuronal toxicity by using cortical neurons from hiPSCs of Alzheimer’s disease patients after adding secondary metabolites.
    [Scientific Reports]

    Full Article

    Learn about standards for PSC banking by watching Dr. Glyn Stacey's webinar from the Nature Research Round Table on hPSC Quality
    REVIEWS

    Microengineered Platforms for Characterizing the Contractile Function of In Vitro Cardiac Models

    The authors discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models, including single cardiomyocytes, 2D monolayers of cardiomyocytes, and 3D cardiac tissues.
    [Microsystems & Nanoengineering]

    Full Article

    INDUSTRY AND POLICY NEWS

    MDimune Signs Research Agreement with YiPSCELL to Develop iPSC-Based BioDrone® Therapeutics

    MDimune announced a joint research agreement with YiPSCELL to develop BioDrone® Platform therapeutics based on iPSCs.
    [MDimune (EIN Presswire, Inc.)]

    Press Release

    FEATURED EVENT

    SSCN Swiss Stem Cell Network

    May 9, 2022
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Postdoctoral Research Associate – Morphogenesis and Differentiation

    University of Edinburgh – Edinburgh, Scotland, United Kingdom

    Postdoctoral Scholar – Cancer Cell Identity

    Oregon Health and Science University – Portland, Oregon, United States

    Postdoctoral Fellow – Diabetes, Cardiovascular Disorders and Non-Alcoholic Steatohepatitis

    NIH National Heart, Lung, and Blood Institute – Bethesda, Maryland, United States

    Postdoctoral Position – Tissue Engineering and Epigenetics

    University of Lausanne – Lausanne, Switzerland

    PhD Position – iPSC-Based Neuronal Transplants

    University Hospital Bonn – Bonn, Germany

    > See All Jobs

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