ESC & iPSC News Volume 17.18 | May 11 2022

May 11, 2022

141

2022-05-11 | ESC 17.18

ESC & iPSC News by STEMCELL Technologies

Vol. 17.18 – 11 May, 2022

TOP STORY

FTO Mediates LINE1 m⁶A Demethylation and Chromatin Regulation in mESCs and Mouse Development

Scientists showed that fat mass and obesity-associated protein (FTO) mediated m⁶A demethylation of long-interspersed element-1 (LINE1) RNA in mouse ESCs, regulating LINE1 RNA abundance and the local chromatin state, which in turn modulated transcription of LINE1-containing genes.
[Science]

Abstract Press Release

Virtual Conference Exhibition: Pluripotent Stem Cells

PUBLICATIONSRanked by the impact factor of the journal

**Activation of Xist by an Evolutionarily Conserved Function of KDM5C Demethylase**

Ectopic expression of mouse and human KDM5C, but not the Y-linked homolog KDM5D, induced Xist in male mouse ESCs (mESCs). Similarly, marsupial Kdm5c but not Kdm5d also upregulated Xist in male mESCs,
[Nature Communications]

Full Article

Preventing Erosion of X-Chromosome Inactivation in Human Embryonic Stem Cells

Female human ESCs (hESCs) often undergo erosion of X-inactivation upon prolonged culture. Researchers investigated the sources of X-inactivation instability by deriving new primed pluripotent hESC lines.
[Nature Communications]

Full Article

Generation of RYBP FLAG-HA Knock-In Human Embryonic Stem Cell Line through CRISPR/Cas9-Mediated Homologous Recombination

To investigate the role of RYBP in human ESCs (hESCs), the authors generated an hESC line with FLAG-HA tag knock-in to RYBP locus through CRISPR/Cas9-mediated homologous recombination.
[Stem Cell Research]

Abstract Full Article

Generation of a Human Embryonic Stem Cell Line (WAe009-a-78) Carrying Homozygous TBX18 Knockout

Using an episomal vector-based CRISPR/Cas9 system, scientists established a homozygous TBX18 knockout human ESC line.
[Stem Cell Research]

Full Article

Caffeic Acid Selectively Eliminates Teratogenic Human-Induced Pluripotent Stem Cells via Apoptotic Cell Death

Investigators suggested that Caffeic acid was effective in preparing safe iPSC-based therapeutic cells without the risk of teratoma formation and DNA damage in normal cells and iPSC-derived differentiated cells.
[Phytomedicine]

Full Article Graphical Abstract

Selective Activation and Down-Regulation of Trk Receptors by Neurotrophins in Human Neurons Co-Expressing TrkB and TrkC

Using neurons differentiated from human ESCs expressing both TrkB and TrkC, researchers compared Trk activation by brain-derived neurotrophic factor and neurotrophin-3.
[Journal of Neurochemistry]

Full Article Graphical Abstract

**Hyperexcitability in Young iPSC-Derived C9ORF72 Mutant Motor Neurons Is Associated with Increased Intracellular Calcium Release**

The authors studied motor neuron cultures derived from iPSC lines generated from C9ORF72 expansion carriers and unaffected controls.
[Scientific Reports]

Full Article

Heterogeneous Nuclear Ribonucleoprotein U-Actin Complex Derived from Extracellular Vesicles Facilitates Proliferation and Migration of Human Coronary Artery Endothelial Cells by Promoting RNA Polymerase II Transcription

Investigators explored the regulation of ESCs-derived-extracellular vesicles-hnRNPU-actin complex in human coronary artery endothelial cell growth.
[Bioengineered]

Full Article Graphical Abstract

Webinar: Advances in Human Brain Organoid Models

REVIEWS

Scalable Manufacturing of Clinical-Grade Differentiated Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells for Regenerative Therapy

Scientists discuss the future directions for realizing hPSC-based cardiac regenerative therapy.
[Cell Proliferation]

Full Article

Cardiac Organoids: A 3D Technology for Modeling Heart Development and Disease

The authors discuss the recent advances in cardiac organoid technologies derived from human ESCs and hiPSCs, with a focus on the summary of methods for organoid generation.
[Stem Cell Reviews and Reports]

Abstract Graphical Abstract

INDUSTRY AND POLICY NEWS

Bit.bio Launches Huntington’s Disease Human Cell Model

Bit.bio launches their iPSC-derived ioGlutamatergic Neurons HTT^50CAG/WT cells to advance research and accelerate drug discovery for Huntington’s disease.
[Bit.bio]

Press Release

FEATURED EVENT

ESC & iPSC News Volume 17.18 | May 11 2022

FTO Mediates LINE1 m⁶A Demethylation and Chromatin Regulation in mESCs and Mouse Development

**Activation of Xist by an Evolutionarily Conserved Function of KDM5C Demethylase**

Preventing Erosion of X-Chromosome Inactivation in Human Embryonic Stem Cells

Generation of RYBP FLAG-HA Knock-In Human Embryonic Stem Cell Line through CRISPR/Cas9-Mediated Homologous Recombination

Generation of a Human Embryonic Stem Cell Line (WAe009-a-78) Carrying Homozygous TBX18 Knockout

Caffeic Acid Selectively Eliminates Teratogenic Human-Induced Pluripotent Stem Cells via Apoptotic Cell Death

Selective Activation and Down-Regulation of Trk Receptors by Neurotrophins in Human Neurons Co-Expressing TrkB and TrkC

**Hyperexcitability in Young iPSC-Derived C9ORF72 Mutant Motor Neurons Is Associated with Increased Intracellular Calcium Release**

Heterogeneous Nuclear Ribonucleoprotein U-Actin Complex Derived from Extracellular Vesicles Facilitates Proliferation and Migration of Human Coronary Artery Endothelial Cells by Promoting RNA Polymerase II Transcription

Scalable Manufacturing of Clinical-Grade Differentiated Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells for Regenerative Therapy

Cardiac Organoids: A 3D Technology for Modeling Heart Development and Disease

Bit.bio Launches Huntington’s Disease Human Cell Model

Danish Stem Cell Society (DASCS) 2022

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FTO Mediates LINE1 m6A Demethylation and Chromatin Regulation in mESCs and Mouse Development

Activation of Xist by an Evolutionarily Conserved Function of KDM5C Demethylase

Preventing Erosion of X-Chromosome Inactivation in Human Embryonic Stem Cells

Generation of RYBP FLAG-HA Knock-In Human Embryonic Stem Cell Line through CRISPR/Cas9-Mediated Homologous Recombination

Generation of a Human Embryonic Stem Cell Line (WAe009-a-78) Carrying Homozygous TBX18 Knockout

Caffeic Acid Selectively Eliminates Teratogenic Human-Induced Pluripotent Stem Cells via Apoptotic Cell Death

Selective Activation and Down-Regulation of Trk Receptors by Neurotrophins in Human Neurons Co-Expressing TrkB and TrkC

Hyperexcitability in Young iPSC-Derived C9ORF72 Mutant Motor Neurons Is Associated with Increased Intracellular Calcium Release

Heterogeneous Nuclear Ribonucleoprotein U-Actin Complex Derived from Extracellular Vesicles Facilitates Proliferation and Migration of Human Coronary Artery Endothelial Cells by Promoting RNA Polymerase II Transcription

Scalable Manufacturing of Clinical-Grade Differentiated Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells for Regenerative Therapy

Cardiac Organoids: A 3D Technology for Modeling Heart Development and Disease

Bit.bio Launches Huntington’s Disease Human Cell Model

FTO Mediates LINE1 m⁶A Demethylation and Chromatin Regulation in mESCs and Mouse Development

**Activation of Xist by an Evolutionarily Conserved Function of KDM5C Demethylase**

**Hyperexcitability in Young iPSC-Derived C9ORF72 Mutant Motor Neurons Is Associated with Increased Intracellular Calcium Release**