 | | Vol. 17.47 – 7 December, 2022 |
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| Researchers showed that combinatorial genetic deletions of all four proteins with functional methyl-CpG-binding domains in mESCs, derived neurons, or a human cell line did not reactivate genes or repeats with methylated promoters.
[Nature Genetics] |
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 | | PUBLICATIONSRanked by the impact factor of the journal |
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| To address why acute depletion of CTCF and cohesin only marginally affected expression of most genes, scientists used high-resolution Micro-C and nascent transcript profiling in mESCs.
[Nature Genetics] |
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| Investigators sub-cloned candidate hiPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types.
[Cell Stem Cell] |
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| Using an unbiased and integrated approach combining microfluidic cultures with transcriptional, proteomic, and secretome analyses, researchers found that naive, but not primed, hiPSC colonies were characterized by a self-organized extracellular matrix-rich microenvironment.
[Cell Stem Cell] |
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| The authors showed that tricarboxylic acid (TCA) cycle enzymes including Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha, and Mdh2 were translocated to the nucleus during somatic cell reprogramming, primed-to-naive transition and totipotency acquisition.
[Nature Communications] |
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| Scientists applied short-read or Oxford Nanopore Technologies long-read genome sequencing to 38 bulk miPSC lines reprogrammed from 10 parental cell types, and 18 single-cell miPSC clones.
[Nature Communications] |
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| Researchers analyzed origin activity in mESCs in the absence or presence of mild replicative stress induced by aphidicolin, a DNA polymerase inhibitor, or by deregulation of origin licensing factor CDC6.
[Nucleic Acids Research] |
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| The authors reported the development of two transgenic mouse lines from which 2° cells reprogram with unprecedented efficiency. These systems were derived by exposing primary reprogramming cells containing doxycycline-inducible Yamanaka factor expression to a transient interruption in transgene expression.
[Proceedings of the National Academy of Sciences of the United States of America] |
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| Sets of time-lapse bright-field images were taken to track the reprogramming process of CD34+ cells biologically identified as just beginning reprogramming.
[Computer Methods and Programs in Biomedicine] |
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| Investigators reported GermLayerTracker, a combination of site-specific DNA methylation assays that served as a biomarker for early germ layer specification.
[Stem Cell Reports] |
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| The authors summarize current knowledge of the nature of recurrent genetic and epigenetic variants in hPSC culture, the methods for their detection, and what is known concerning their effects on cell behavior in vitro or in vivo.
[Cell Stem Cell] |
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| MDimune Inc. announced a research collaboration agreement with the Clear Vision Research Lab at the Australian National University.
[MDimune Inc. (BioSpace.com.)] |
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| June 5 – 8, 2023
Winston-Salem, North Carolina, United States |
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| | Boston Children’s Hospital – Boston, Massachusetts, United States |
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| | Max Delbrück Center for Molecular Medicine – Berlin-Buch, Germany |
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| | University of Bristol – Bristol, England, United Kingdom |
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| | City of Hope – Los Angeles, California, United States |
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| | University of Edinburgh – Edinburgh, Scotland, United Kingdom |
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