Somatic Coding Mutations in Human Induced Pluripotent Stem Cells Researchers show that 22 human induced pluripotent stem cell lines reprogrammed using five different methods each contained an average of five protein-coding point mutations in the regions sampled (an estimated six protein-coding point mutations per exome). [Nature] Short-Term Immunosuppression Promotes Engraftment of Embryonic and Induced Pluripotent Stem Cells This report describes a short-term immunosuppressive approach capable of inducing engraftment of transplanted embryonic stem cells and induced pluripotent stem cells, providing a significant improvement in our mechanistic understanding of the critical role costimulatory molecules play in leukocyte activation. [Cell Stem Cell] LRRK2 Mutant iPSC-Derived DA Neurons Demonstrate Increased Susceptibility to Oxidative Stress Researchers report generation of induced pluripotent stem cells that carry the p.G2019S mutation (G2019S-iPSCs) in the Leucine-Rich Repeat Kinase-2 (LRRK2) gene, the most common Parkinson’s disease-related mutation, and their differentiation into dopaminergic (DA) neurons. [Cell Stem Cell] Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm Prospective isolation, lineage tracing, and developmental studies of SOX17+ human embryonic stem cell progeny have revealed fundamental aspects of human endodermal biology. [Cell Stem Cell] FGF2 Sustains NANOG and Switches the Outcome of BMP4-Induced Human Embryonic Stem Cell Differentiation Results demonstrate that FGF2 signaling switches the outcome of BMP4-induced differentiation of human embryonic stem cells by maintaining NANOG levels through the MEK-ERK pathway. [Cell Stem Cell] Isolation of Epiblast Stem Cells from Preimplantation Mouse Embryos Researchers show that epiblast stem cells can be routinely derived from pre-implantation mouse embryos. [Cell Stem Cell] A Bioinformatic Assay for Pluripotency in Human Cells Researchers report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles. [Nat Methods] Defining the Earliest Step of Cardiovascular Progenitor Specification During Embryonic Stem Cell Differentiation The study identifies the early multipotent cardiovascular progenitors residing at the top of the cellular hierarchy of cardiovascular lineages during embryonic stem cell differentiation. [J Cell Biol] Self-Renewal Induced Efficiently, Safely, and Effective Therapeutically with One Regulatable Gene in a Human Somatic Progenitor Cell Precursor/progenitor cells from the organ of therapeutic concern typically lack only one critical attribute – the capacity for sustained self-renewal. Researchers speculated that this could be induced in a regulatable manner such that cells proliferate only in vitro and differentiate in vivo without the need for promoting pluripotence or specifying lineage identity. [Proc Natl Acad Sci U S A] The Controlled Generation of Functional Basal Forebrain Cholinergic Neurons from Human Embryonic Stem Cells Researchers demonstrate here a method for the derivation of a predominantly pure population of basal forebrain cholinergic neurons from human embryonic stem cells using diffusible ligands present in the forebrain at developmentally relevant time periods. [Stem Cells] Assessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-Induced Embryonic Stem Cell-Derived Progenitors Researchers show that Pax7, like Pax3, can drive the expansion of an embryonic stem-derived myogenic progenitor with significant muscle regenerative potential. [Stem Cells] |