Defining the Earliest Step of Cardiovascular Progenitor Specification During Embryonic Stem Cell Differentiation This study identifies the early multipotent cardiovascular progenitors residing at the top of the cellular hierarchy of cardiovascular lineages during embryonic stem cell differentiation. [J Cell Biol] Snail and the miR-200 Family Act in Opposition to Regulate EMT and Germ Layer Fate Restriction in Differentiating ES Cells Researchers show that Snail homolog 1 (Snail) is expressed during embryonic stem (ES) cell differentiation and is capable of inducing epithelial-to-mesenchymal transition (EMT) on day 2 of ES cell differentiation. [Stem Cells] Direct In Vivo Cellular Reprogramming Involves Transition Through Discrete, Non-Pluripotent Steps Findings suggest that direct cell type conversion occurs via successive steps, and that dedifferentiation can occur in the absence of cell division. Furthermore, the results suggest that mechanisms are in place in vivo to restrict cell potential during reprogramming, a finding with important implications for regenerative medicine. [Development] Regulation of Mouse Embryonic Stem Cell Self-Renewal by a Yes-YAP-TEAD2 Signaling Pathway Downstream of LIF Researchers show that YAP, TEAD2 and Yes are highly expressed in self-renewing embryonic stem cells, are activated by leukemia inhibitory factor (LIF) and serum, and are downregulated when cells are induced to differentiate. [J Cell Sci] REST Interacts with Cbx Proteins and Regulates Polycomb Repressive Complex 1 Occupancy at RE1 Elements Researchers found that REST and nine REST-associated proteins co-purified with Cbx family Polycomb group proteins from mouse embryonic stem cells. [Mol Cell Biol] Embryonic Stem Cells, Derived Either After In Vitro Fertilization or Nuclear Transfer, Prolong Survival of Semiallogeneic Heart Transplants Researchers investigated the immunomodulatory properties of murine embryonic stem cells, obtained either by in vitro fertilization or by nuclear transfer, in heart transplant mouse models. [J Immunol] Musashi 1 Cooperates in Abnormal Cell LINeage Protein 28 (Lin28)-Mediated Let-7 Family MicroRNA Biogenesis in Early Neural Differentiation Results suggest that Musashi1 can influence stem-cell maintenance and differentiation by controlling the subcellular localization of proteins involved in microRNA biogenesis, as well as by regulating the translation of its target mRNA. [J Biol Chem] Efficient Derivation and Concise Gene Expression Profiling of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors (EMPs) Researchers report a simple and efficient method of generating human embryonic stem cell-derived mesenchymal progenitors and transcriptome profiling using a concise, custom-designed, oligomnucleotide gene expression microarray. [Cell Transplant] DNA Methylation Assay for X-Chromosome Inactivation in Female Human Induced Pluripotent Stem Cells Researchers provide a detailed protocol for a simple, reliable and affordable X-inactivation assay based on promoter methylation and CAG-repeat polymorphism in the human androgen receptor gene at Xq11.2. [Stem Cell Rev] Assessment of Differentiation Aspects by the Morphological Classification of Embryoid Bodies Derived from Human Embryonic Stem Cells Researchers sought to classify the differentiation potentials of embryoid bodies by morphologies to enable one to control the differentiation of specific lineages from human embryonic stem cells with high efficiency. [Stem Cells Dev] |