Dynamic Regulation of 5-Hydroxymethylcytosine in Mouse Embryonic Stem Cells and During Differentiation Researchers propose that the balance between hydroxymethylation and methylation in the genome is inextricably linked with the balance between pluripotency and lineage commitment. [Nature] Dual Functions of Tet1 in Transcriptional Regulation in Mouse Embryonic Stem Cells The study not only establishes a role for Tet1 in modulating DNA methylation levels at CpG-rich promoters, but also reveals a dual function of Tet1 in promoting transcription of pluripotency factors as well as participating in the repression of Polycomb-targeted developmental regulators. [Nature] Mutant Human Embryonic Stem Cells Reveal Neurite and Synapse Formation Defects in Type 1 Myotonic Dystrophy By using neuronal progeny derived from human embryonic stem cells carrying the causal myotonic dystrophy type 1 mutation, researchers have identified an early developmental defect in genes involved in neurite formation and the establishment of neuromuscular connections. [Cell Stem Cell] A More Efficient Method to Generate Integration-Free Human iPS Cells Researchers report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. [Nat Methods] Genome-Wide Analysis of 5-Hydroxymethylcytosine Distribution Reveals Its Dual Function in Transcriptional Regulation in Mouse Embryonic Stem Cells Researchers present a genome-wide profile of 5-hydroxymethylcytosine in mouse embryonic stem cells. [Genes Dev] Genetic Correction and Analysis of Induced Pluripotent Stem Cells from a Patient with Gyrate Atrophy Findings confirm that induced pluripotent stem cells themselves may carry a significant mutational load at initial isolation, but that the clonal events and prolonged cultured required for correction of a genetic defect can be accomplished without a substantial increase in mutational burden. [Proc Natl Acad Sci U S A] Generation of Stable Pluripotent Stem Cells From NOD Mouse Tail-Tip Fibroblasts The objective of this study was to determine whether somatic fibroblasts from NOD mice could be reprogrammed to become induced pluripotent stem cells, providing an alternative source of stem cells for the production of genetically modified NOD cells and mice. [Diabetes] One-Dimensional Self-Assembly of Mouse Embryonic Stem Cells Using an Array of Hydrogel Microstrands Researchers synthesized an array of one-dimensional alginate gel microstrands and aqueous microstrands through an SU-8 filter device by means of capillary action. Furthermore, they investigated self-assembly behaviors and differentiation potentials of mouse embryonic stem cells cultured in microstrands of varying diameters. [Biomaterials] Feeder-Free Derivation of Induced Pluripotent Stem Cells from Human Immature Dental Pulp Stem Cells Data demonstrate that human immature dental pulp stem cell-induced pluripotent stem cells offer an advantageous cell system for future cell therapy and basic studies, particularly as a model for pediatric developmental disorders. [Cell Transplant] Differentiation of Human Embryonic Stem Cell-Derived Retinal Progenitors into Retinal Cells by Sonic Hedgehog and/or Retinal Pigmented Epithelium and Transplantation into the Subretinal Space of Sodium Iodate-Injected Rabbits Researchers differentiated initially retinal progenitors from adherent feeder-free human embryonic stem cells with the use of defined media supplemented with a specific combination of growth factors. [Stem Cells Dev] |