Derivation and Propagation of Human Embryonic Stem Cell Lines from Frozen Embryos in an Animal Product-Free Environment The protocols described are comprehensive instructions for deriving human embryonic stem cell lines in xeno-free conditions from cryopreserved embryos. [Nat Protoc] Abstract Global Identification of Transcriptional Regulators of Pluripotency and Differentiation in Embryonic Stem Cells Investigators proposed a systematic approach to integrate genomic and epigenomic data for identification of direct regulatory interactions. This approach allows reconstruction of cell-type-specific transcription networks in embryonic stem cells and fibroblasts at an unprecedented scale. [Nucleic Acids Res] Full Article Human Induced Pluripotent Stem Cells Can Reach Complete Terminal Maturation: In Vivo and In Vitro Evidence in the Erythropoietic Differentiation Model Researchers demonstrated that human induced pluripotent stem cells can achieve complete terminal erythroid maturation and open the way to generation of functionally corrected red blood cells from sickle cell disease induced pluripotent stem cells, without any genetic modification or drug treatment. [Haematologica] Abstract | Full Article Human Umbilical Cord Mesenchymal Stem Cells Support Non-Tumorigenic Expansion of Human Embryonic Stem Cells Researchers tested human umbilical cord-derived mesenchymal stem cells as the feeder for human embryonic stem cells. [Cell Transplant] Abstract Programming of Regulatory T Cells from Pluripotent Stem Cells and Prevention of Autoimmunity Investigators showed how to generate functional regulatory T (Treg) cells from induced pluripotent stem cells and to determine the potential role of such cells for Treg cell-based immunotherapy against autoimmunity in a therapeutic setting. [J Immunol] Abstract Efficient Engineering of Vascularized Ectopic Bone from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells Scientists have developed efficient and reproducible directed differentiation systems toward human mesenchymal stem cells from human embryonic stem cells, including co-culture with murine OP9 stromal cells and feeder layer-free. [Tissue Eng Part A] Abstract Bisphenol A Modulates Germ Cell Differentiation and Retinoic Acid Signaling in Mouse ES Cells Researchers investigated the effect of bisphenol A using in vitro differentiation of embryonic stem (ES) cells focusing on the expression of germ cell marker genes. [Reprod Toxicol] Abstract Trim71 Cooperates with MicroRNAs to Repress Cyclin-Dependent Kinase Inhibitor 1a Expression and Promote Embryonic Stem Cell Proliferation Scientists demonstrated that tripartite motif-containing protein 71 (Trim71) is a factor that facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal. [Nat Commun] Abstract |