Human Embryonic Stem Cell-Derived Hemogenic Endothelial Cells Undergo Distinct Waves of Endothelial to Hematopoietic Transition Several studies have demonstrated that hematopoietic cells originate from endothelium in early development; however, the phenotypic progression of progenitor cells during human embryonic hemogenesis is not well described. Researchers defined the developmental hierarchy among intermediate populations of hematopoietic progenitor cells derived from human embryonic stem cells. [Blood] Abstract Role of SOX17 in Hematopoietic Development from Human Embryonic Stem Cells To search for genes that promote hematopoietic development from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), researchers overexpressed several known hematopoietic regulator genes in hESCs/iPSCs-derived CD34+CD43– endothelial cells enriched in hemogenic endothelium. [Blood] Abstract Reverse Engineering a Mouse Embryonic Stem Cell-Specific Transcriptional Network Reveals a New Modulator of Neuronal Differentiation The overexpression and knock-down of E130012A19Rik in embryonic stem cell lines undergoing differentiation into neurons and glia cells caused a strong up-regulation of the glutamatergic neurons marker Vglut2 and a strong down-regulation of the GABAergic neurons marker GAD65 and of the radial glia marker Blbp. [Nucleic Acids Res] Full Article Transcription Elongation Factor Tcea3 Regulates the Pluripotent Differentiation Potential of Mouse Embryonic Stem Cells, via the Lefty1-Nodal-Smad2 Pathway The authors report that the transcription elongation factor Tcea3 is highly enriched in mouse embryonic stem cells (ESCs) and plays important roles in regulating the differentiation. Strikingly, altering Tcea3 expression in mouse ESCs did not affect self-renewal under non-differentiating conditions; however, upon exposure to differentiating cues, its overexpression impaired in vitro differentiation capacity, and its knockdown biased differentiation towards mesodermal and endodermal fates. [Stem Cells] Abstract Intrapatient Variations in Type 1 Diabetes-Specific Induced Pluripotent Stem Cell Differentiation into Insulin-Producing Cells From individual patients with type 1 diabetes, multiple human induced pluripotent clones were produced and prospectively screened using a battery of developmental markers to assess respective differentiation propensity and proficiency in yielding functional insulin-producing progeny. [Mol Ther] Abstract Human Embryonic Stem Cell-Derived Test Systems for Developmental Neurotoxicity: a Transcriptomics Approach The fields of developmental neurotoxicity (DNT) and reproductive toxicity (RT) would greatly benefit from in vitro tests that allow the identification of toxicant-induced changes of the cellular proteostasis, or of its underlying transcriptome network. Therefore, the ‘human embryonic stem cell (hESC)-derived novel alternative test systems’ European commission research project established RT tests based on defined differentiation protocols of hESC and their progeny. In this study, valproic acid and methylmercury were used as positive control compounds to address fundamental questions. [Arch Toxicol] Full Article HMGA1 Reprograms Somatic Cells into Pluripotent Stem Cells by Inducing Stem Cell Transcriptional Networks Researchers demonstrated for the first time that high mobility group A1 (HMGA1) enhances cellular reprogramming from a somatic cell to a fully pluripotent stem cell. These findings identify a novel role for HMGA1 as a key regulator of the stem cell state by inducing transcriptional networks that drive pluripotency. [PLoS One] Full Article Keratinocyte Growth Factor and Dexamethasone Plus Elevated cAMP Levels Synergistically Support Pluripotent Stem Cell Differentiation into Alveolar Epithelial Type II Cells Alveolar epithelial type II (ATII)-like cells can be generated from murine embryonic stem cells (ESCs), albeit to date no robust protocols applying specific differentiation factors are established. Scientists hypothesized that keratinocyte growth factor, an important mediator of lung organogenesis and primary ATII cell maturation and proliferation, together with dexamethasone, 8-bromoadenosine-cAMP and isobutylmethylxanthine, which induce maturation of primary fetal ATII cells, also support the alveolar differentiation of murine ESCs. [Tissue Eng Part A] Abstract Injectable Polyethylene Glycol-Fibrinogen Hydrogel Adjuvant Improves Survival and Differentiation of Transplanted Mesoangioblasts in Acute and Chronic Skeletal Muscle Degeneration Researchers investigated the use of intra-muscular transplantation of mesoangioblasts (vessel-associated progenitor cells), delivered with an injectable hydrogel biomaterial directly into the tibialis anterior muscle of acutely injured or dystrophic mice. The hydrogel cell carrier, made from a polyethylene glycol-fibrinogen matrix, is polymerized in situ together with mesoangioblasts to form a resorbable cellularized implant. [Skelet Muscle] Full Article | Press Release |