High Dub3 Expression in Mouse ESCs Couples the G1/S Checkpoint to Pluripotency Investigators observed high Cdc25A levels in G1 that persist even after DNA damage in mouse embryonic stem cells (ESCs). They also found higher expression of Dub3, a deubiquitylase that controls Cdc25A protein abundance. Moreover, they demonstrated that the Dub3 gene is a direct target of Esrrb, a key transcription factor of the self-renewal machinery. [Mol Cell] Abstract | Graphical Abstract Isogenic Human Pluripotent Stem Cell Pairs Reveal the Role of a KCNH2 Mutation in Long-QT Syndrome To study the LQT2-associated c.A2987T KCNH2 mutation under genetically defined conditions, the authors derived induced pluripotent stem cells from a patient carrying this mutation and corrected it. Furthermore, they introduced the same point mutation in human embryonic stem cells, generating two genetically distinct isogenic pairs of long-QT syndromes (LQTS) and control lines. [EMBO J] Full Article A Conserved Oct4/POUV-Dependent Network Links Adhesion and Migration to Progenitor Maintenance The authors examined the function of Oct4 homologs in Xenopus embryos and compared this to the role of Oct4 in maintaining mammalian embryo-derived stem cells. Based on a combination of expression profiling of Oct4/POUV-depleted Xenopus embryos and in silico analysis of existing mammalian Oct4 target data sets, they defined a set of evolutionary-conserved Oct4/POUV targets. [Curr Biol] Abstract | Graphical Abstract | Press Release Beta Cell Dysfunction Due to Increased ER Stress in a Stem Cell Model of Wolfram Syndrome To investigate the cause of beta cell failure, researchers used induced pluripotent stem cells to create insulin-producing cells from individuals with Wolfram syndrome. WFS1-deficient beta cells showed increased levels of endoplasmic reticulum (ER) stress molecules, and decreased insulin content. [Diabetes] Abstract | Press Release Identification of Ccr4-Not Complex Components as Regulators of Transition from Partial to Genuine Induced Pluripotent Stem Cells Researchers performed genome-wide screening with a piggyBac vector to identify genes involved in conversion from partial to genuine induced pluripotent stem cells. This screening led to identification of Cnot2, one of the core components of the Ccr4-Not complex. [Stem Cells Dev] Abstract Gain of 20q11.21 in Human Embryonic Stem Cells Improves Cell-Survival by Increased Expression of Bcl-xL Scientists studied three human embryonic stem cell lines that acquired a gain of 20q11.21 during in vitro culture. The study of the mRNA gene expression levels of the loci located in the common region of duplication showed that HM13, ID1, BCL2L1, KIF3B and the immature form of the micro RNA miR-1825 were up-regulated in mutant cells. [Mol Hum Reprod] Abstract A Modified TALEN-Based System for Robust Generation of Knock-Out Human Pluripotent Stem Cell Lines and Disease Models The modular architecture of TAL effectors theoretically enables targeting of any genomic locus and several cloning systems for custom transcription activator-like effector nuclease (TALEN) assembly have recently been established. However, there is a lack of versatile TALEN expression systems applicable to human pluripotent stem cells (hPSCs). Here, the authors extend an existing TALE assembly system by a dual set of expression vectors for efficient application of TALEN technology in hPSCs. [BMC Genomics] Abstract | Full Article Functional Differences in Engineered Myocardium from Embryonic Stem Cell-Derived versus Neonatal Cardiomyocytes Scientists built laminar myocardium engineered from cardiomyocytes that were differentiated from mouse embryonic stem cell-derived cardiac progenitors or harvested directly from neonatal mouse ventricles, and compared their anatomy and physiology in vitro. [Stem Cell Rep] Full Article | Graphical Abstract |