N6-Methyladenosine Modification Destabilizes Developmental Regulators in Embryonic Stem Cells Researchers identified two proteins, the putative N6-methyladenosine (m6A) MTase, methyltransferase-like 3 (Mettl3), and a related but uncharacterized protein Mettl14, that function synergistically to control m6A formation in mammalian cells. Knockdown of Mettl3 and Mettl14 in mouse embryonic stem cells led to similar phenotypes, characterized by lack of m6A RNA methylation and lost self-renewal capability. [Nat Cell Biol] Abstract High-Throughput Sequencing Reveals the Disruption of Methylation of Imprinted Gene in Induced Pluripotent Stem Cells Researchers found that methylation of an imprinted gene, Zrsr1, was consistently disrupted in the induced pluripotent stem cell lines with reduced pluripotency. [Cell Res] Abstract Modeling and Rescuing Neurodevelopmental Defect of Down Syndrome Using Induced Pluripotent Stem Cells from Monozygotic Twins Discordant for Trisomy 21 Researchers report the generation and characterization of induced pluripotent stem cells derived from monozygotic twins discordant for trisomy 21 in order to eliminate the effects of the variability of genomic background. [EMBO Mol Med] Abstract Inhibition of Stearoyl-CoA Desaturase Selectively Eliminates Tumorigenic Nanog-Positive Cells: Improving the Safety of iPS Cell Transplantation to Myocardium The authors found that plurisin#1 treatment significantly decreased the mRNA and protein level of Nanog, a marker for both cell pluripotency and tumor progression; importantly, they provide evidence that PluriSin#1 treatment at 20 µM for 1 day significantly induces the apoptosis of Nanog-positive induced pluripotent stem (iPS) cell derivates. [Cell Cycle] Full Article Identification of a Novel Gene Signature of ES Cells Self-Renewal Fluctuation through System-Wide Analysis Investigators aimed to characterize the high-pluripotent Embryonic Stem (ES) cell metastate marked by the expression of Zscan4 through a supervised machine learning framework based on an ensemble of support vector machine classifiers. [PLoS One] Full Article Generation of iPSC Lines from Archived Non-Cryoprotected Biobanked Dura Mater Researchers report the generation of induced pluripotent stem cells (iPSCs) from frozen non-cryoprotected dural tissue stored at -80°C for up to 11 years. This autopsy cohort included subjects with Alzheimer’s disease and four other neurodegenerative diseases. [Acta Neuropathol Commun] Abstract | Full Article | Press Release PI3K/Akt1 Signaling Specifies Foregut Precursors by Generating Regionalized Extra-Cellular Matrix The authors used mouse embryonic stem cell differentiation to uncover a new mechanism for PI3K signaling that is required for endoderm specification. They found that PI3K signaling promotes the transition from naïve endoderm precursors into committed anterior endoderm. [eLife] Full Article | Press Release Myogenic Differentiation of Muscular Dystrophy-Specific Induced Pluripotent Stem Cells for Use in Drug Discovery Researchers have a particular interest in modeling skeletal muscle from various genetic backgrounds; however, efficient and reproducible methods for the myogenic differentiation of induced pluripotent stem cells have not previously been demonstrated. [Stem Cell Transl Med] Abstract Pluripotent Stem Cells Derived from Mouse and Human White Mature Adipocytes Scientists defined stem cell characteristics of dedifferentiated fat cells as they are generated from adipocytes and the relationship between these characteristics and lineage differentiation. [Stem Cell Transl Med] Abstract |