The Histone H2A Deubiquitinase Usp16 Regulates Embryonic Stem Cell Gene Expression and Lineage Commitment Researchers report that Usp16, a histone H2A deubiquitinase, regulates H2A deubiquitination and gene expression in embryonic stem cells (ESCs), and importantly, is required for ESC differentiation. [Nat Commun] Abstract Rescue of Embryonic Stem Cells from Cellular Transformation by Proteomic Stabilization of Mutant p53 and Conversion into WT Conformation Investigators found that, despite harboring mutant p53, ES cells (ESCs) remain pluripotent and benign and have relatively normal karyotype compared with ESCs knocked out for p53. [Proc Natl Acad Sci USA] Abstract 3D Mouse Embryonic Stem Cell Culture for Generating Inner Ear Organoids Scientists designated the stem cell-derived epithelia harboring hair cells, supporting cells and sensory-like neurons as inner ear organoids. This method provides a reproducible and scalable means to generate inner ear sensory tissue in vitro. [Nat Protoc] Abstract Optimizing Human Embryonic Stem Cells Differentiation Efficiency by Screening Size-Tunable Homogenous Embryoid Bodies Investigators fabricated size-tunable concave microwells to control the physical environment, thereby regulating the size of embryoid bodies formed from single human embryonic stem cells. [Biomaterials] Abstract Induced Pluripotent Stem Cell-Derived Hepatocytes and Endothelial Cells in Multi-Component Hydrogel Fibers for Liver Tissue Engineering Previously, the authors developed methods of encapsulating cells within separate domains in multi-component hydrogel fibers and methods of assembling fibers to form 3D-patterned tissue constructs. They combined these approaches to encapsulate hepatocytes and endothelial cells within their specific niches, and to assemble them into endothelialized liver tissue constructs. [Biomaterials] Abstract Embryonic Stem Cell Therapy Improves Bone Quality in a Model of Impaired Fracture Healing in the Mouse; Tracked Temporally Using In Vivo Micro-CT Investigators used an estrogen-deficient mouse model of osteoporosis to test the efficacy of a cell-generated bone tissue construct for bone augmentation of an impaired healing fracture. A reduction in new bone formation at the defect site was observed in ovariectomized fractures compared to the control group using repeated measures in vivo micro-computed tomography (micro-CT) imaging over four weeks. [Bone] Abstract Recombinase-Mediated Reprogramming and Dystrophin Gene Addition in mdx Mouse Induced Pluripotent Stem Cells Researchers employed non-viral, plasmid-mediated methods to reprogram mdx fibroblasts, using phiC31 integrase to insert a single copy of the reprogramming genes at a safe location in the genome. [PLoS One] Full Article Functional Evaluation of ES-Somatic Cell Hybrids In Vitro and In Vivo Researchers determined that the somatic genome is actively transcribed by generating ES-somatic cell hybrids using Rag2-deficient embryonic stem cells (ESCs) fused to autologous wild-type somatic cells. [Cell Reprogram] Abstract Fate of iPSCs Derived from Azoospermic and Fertile Men following Xenotransplantation to Murine Seminiferous Tubules Undifferentiated induced pluripotent stem cells (iPSCs) transplanted directly into murine seminiferous tubules differentiated extensively to germ-cell-like cells that localized near the basement membrane, demonstrated morphology indistinguishable from fetal germ cells, and expressed germ-cell-specific proteins diagnostic of primordial germ cells. [Cell Rep] Full Article | Graphical Abstract | Press Release |