The Dynamics of DNA Methylation Fidelity during Mouse Embryonic Stem Cell Self-Renewal and Differentiation Scientists showed that methylation fidelity increases globally during differentiation of mouse embryonic stem cells, and is particularly high in the promoter regions of actively expressed genes and positively correlated with active histone modification marks and binding of transcriptional factors. [Genome Res] Abstract | Full Article Tet Oxidizes Thymine to 5-Hydroxymethyluracil in Mouse Embryonic Stem Cell DNA Scientists synthesized isotopologs of all major oxidized pyrimidine and purine bases and performed quantitative MS to show that ten eleven translocation (Tet)-induced oxidation is not limited to 5-methylcytosine but that thymine is also a substrate that gives 5-hydroxymethyluracil in mouse embryonic stem cells. [Nat Chem Biol] Abstract | Press Release Physiological and Ultrastructural Features of Human Induced Pluripotent and Embryonic Stem Cell-Derived Skeletal Myocytes In Vitro Investigators showed that the electrophysiological properties of human induced pluripotent stem-derived skeletal myocytes are strictly similar to those of their embryonic stem cell counterparts, and both are typical of aneural mammalian skeletal muscle. [Proc Natl Acad Sci USA] Abstract Small Molecule Compound Induces Chromatin De-Condensation and Facilitates Induced Pluripotent Stem Cell Generation Researchers report the identification of a novel chemical, CYT296, which improves OSKM-mediated induction of induced pluripotent stem cells over tenfold and enables efficient reprogramming with only Oct4 in combination with other small molecules. [J Mol Cell Biol] Abstract Mutation Frequency Dynamics in HPRT Locus in Culture Adapted Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Correspond to Their Differentiated Counterparts Scientists showed that the increase in the mutation frequency caused by the inhibition of base excision repair was similar to that caused by the human embryonic stem cell adaptation process. [Stem Cell Dev] Abstract Genetic and Chemical Correction of Cholesterol Accumulation and Impaired Autophagy in Hepatic and Neural Cells Derived from Niemann-Pick Type C Patient-Specific iPS Cells Researchers report the generation of isogenic pairs of Niemann-Pick type C patient-specific induced pluripotent stem (iPS) cells using transcription activator-like effector nucleases. [Stem Cell Rep] Full Article | Graphical Abstract | Press Release Path to the Clinic: Assessment of iPSC-Based Cell Therapies In Vivo in a Nonhuman Primate Model Scientists developed a clinically relevant nonhuman primate model to assess the tumorigenic potential and in vivo efficacy of both undifferentiated and differentiated induced pluripotent stem cells (iPSCs) in autologous settings without immunosuppression. [Cell Rep] Full Article | Graphical Abstract | Press Release Stable Enhanced Green Fluorescent Protein Expression after Differentiation and Transplantation of Reporter Human Induced Pluripotent Stem Cells Generated by AAVS1 Transcription Activator-Like Effector Nucleases It is not clear whether targeted transgene expression at the adeno-associated virus integration site 1 (AAVS1) locus is always protected from silencing when driven by various promoters, especially after differentiation and transplantation from human induced pluripotent stem cells. Investigators described a pair of transcription activator-like effector nucleases that enable more efficient genome editing than the commercially available zinc finger nuclease at the AAVS1 site. [Stem Cells Transl Med] Abstract |