Loss of Transcriptional Control over Endogenous Retroelements during Reprogramming to Pluripotency Scientists asked how endogenous retroelement control is maintained during the generation of induced pluripotent stem cells, as this procedure involves profound epigenetic remodeling. [Genome Res] Abstract Generation of Organized Germ Layers from a Single Mouse Embryonic Stem Cell Researchers present a method of generating organized germ layers from a single mouse embryonic stem cell cultured in a soft fibrin matrix. Spatial organization of germ layers is regulated by cortical tension of the colony, matrix dimensionality and softness, and cell-cell adhesion. [Nat Commun] Full Article | Press Release Differential Role of Nonhomologous End Joining Factors in the Generation, DNA Damage Response, and Myeloid Differentiation of Human Induced Pluripotent Stem Cells Repair of DNA double-strand breaks is important for reprogramming of somatic cells into induced pluripotent stem cells. To compare the specific contribution of DNA ligase 4, Artemis, and DNA-protein kinase catalytic subunit in this process and to gain insights into phenotypic variability associated with these disorders, researchers reprogrammed patient-derived fibroblast cell lines with nonhomologous end-joining defects. [Proc Natl Acad Sci USA] Abstract From Confluent Human iPS Cells to Self-Forming Neural Retina and Retinal Pigmented Epithelium Scientists developed a simple retinal differentiation method, based on confluent human induced pluripotent stem (iPS) cells, bypassing embryoid body formation and the use of exogenous molecules, coating, or Matrigel. [Proc Natl Acad Sci USA] Abstract Reprogramming of Mouse Somatic Cells into Pluripotent Stem-Like Cells Using a Combination of Small Molecules Somatic cells can be reprogrammed to generate induced pluripotent stem (iPS) cells by overexpression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc. However, exogenous expression of pluripotency factors raised concerns for clinical applications. Investigators showed that iPS-like cells were generated from mouse somatic cells in two steps with small molecule compounds. [Biomaterials] Abstract The Effects of Triclosan on Pluripotency Factors and Development of Mouse Embryonic Stem Cells and Zebrafish Mouse embryonic stem cells were acutely exposed to triclosan (TCS) for 24 hours, and general cytotoxicity and the effect of TCS on pluripotency were then evaluated. [Arch Toxicol] Abstract Conversion of Partially Reprogrammed Cells to Fully Pluripotent Stem Cells Is Associated with Further Activation of Stem Cell Maintenance- and Gamete Generation-Related Genes Researchers generated induced pluripotent stem cells from mouse embryonic fibroblasts (with Oct4-GFP reporter) by transfection of pCX-OSK-2A (Oct4, Sox2, and Klf4) and pCX-cMyc vectors. [Stem Cells Dev] Abstract MicroRNA-Mediated Survivin Control of Pluripotency Because the protein survivin is uniquely overexpressed in virtually all human cancers and in human embryonic stem cells, scientists sought to investigate its role in supporting the distinctive capabilities of these cell types. [J Cell Physiol] Abstract A Multiplex High-Throughput Gene Expression Assay to Simultaneously Detect Disease and Functional Markers in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Investigators used a multiplex high-throughput gene expression assay that simultaneously detects endogenous expression of multiple developmental, functional, and disease markers in induced pluripotent stem cell-derived retinal pigment epithelium. [Stem Cells Transl Med] Abstract |