A Method to Recapitulate Early Embryonic Spatial Patterning in Human Embryonic Stem Cells Scientists show that geometric confinement is sufficient to trigger self-organized patterning in human embryonic stem cells. In response to BMP4, colonies reproducibly differentiated to an outer trophectoderm-like ring, an inner ectodermal circle and a ring of mesendoderm expressing primitive-streak markers in between. [Nat Methods] Abstract | Press Release Low-Grade Chromosomal Mosaicism in Human Somatic and Embryonic Stem Cell Populations Scientists studied the genetic content of 92 individual human cells, including fibroblasts, amniocytes and embryonic stem cells, using single-cell array-based comparative genomic hybridization. [Nat Commun] Abstract Suv39h-Dependent H3K9me3 Marks Intact Retrotransposons and Silences LINE Elements in Mouse Embryonic Stem Cells Scientists dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP sequencing in mouse embryonic stem cells (ESCs). Refined bioinformatic analyses of repeat subfamilies indicated selective accumulation of Suv39h-dependent H3K9me3 at interspersed repetitive elements that cover ~5% of the ESC epigenome. [Mol Cell] Abstract | Graphical Abstract CstF-64 Supports Pluripotency and Regulates Cell Cycle Progression in Embryonic Stem Cells through Histone 3′ End Processing The role of 3′ end processing in regulation of embryonic stem cell (ESC) pluripotency and cell cycle is poorly understood. To better understand these processes, investigators examined mouse ESCs that lack the 3′ end-processing factor CstF-64. [Nucleic Acids Res] Full Article Passage Number Is a Major Contributor to Genomic Structural Variations in Mouse iPS Cells The authors studied the extent of genomic variation on the scale of individual cells originating from the same animal. They used mouse neural stem cells grown from embryonic cells and induced pluripotent stem (iPS) cells generated from embryonic brain cells, B cells or fibroblasts, and performed comparative analysis with cultures of fibroblasts from the same mouse. [Stem Cells] Abstract Generation of Insulin-Producing β-Like Cells from Human iPS Cells in a Defined and Completely Xeno-Free Culture System Scientists developed a five-step xeno-free culture system to efficiently differentiate human induced pluripotent stem (iPS) cells into insulin-producing cells in vitro. [J Mol Cell Biol] Abstract Antiviral Responses in Mouse Embryonic Stem Cells: Differential Development of Cellular Mechanisms in Type I Interferon Production and Response Researchers report that mouse embryonic stem cells are able to respond to type-I interferons (IFNs), express IFN-stimulated genes, and mediate the antiviral effect of type-I IFNs against La Crosse virus and chikungunya virus. [J Biol Chem] Abstract | Full Article Dual Gene Expression in Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells Using Episomal Vectors Scientists sought to establish an efficient method of transfection and rotary culture to generate embryoid bodies that stably express two genes. The pMetLuc2-Reporter vector was transfected using FuGENE HD, Lipofectamine LTX, X-tremeGENE, or TransIT-2020 transfection reagents. [Tissue Eng Part A] Abstract Derivation and Long-Term Culture of an Embryonic Stem Cell-Like Line from Zebrafish Blastomeres under Feeder-Free Condition Scientists describe the derivation and long-term culture of an embryonic stem cell-like line derived from zebrafish blastomeres without the use of feeder cells. [Zebrafish] Full Article | Press Release Antitumor Effects of CD40 Ligand-Expressing Endothelial Progenitor Cells Derived from Human Induced Pluripotent Stem Cells in a Metastatic Breast Cancer Model Scientists sought to explore whether human induced pluripotent stem cells could be used as a reliable and accessible cell source to generate human endothelial progenitor cells suitable for cancer treatment. [Stem Cells Transl Med] Abstract |