Generation of the Epicardial Lineage from Human Pluripotent Stem Cells Researchers describe the generation of epicardial lineage cells from human pluripotent stem cells by stage-specific activation of the BMP and WNT signaling pathways. [Nat Biotechnol] Abstract A Cell Engineering Strategy to Enhance the Safety of Stem Cell Therapies Using a cell engineering approach, investigators show that aggressive cancers derived from human or murine induced pluripotent stem cells and embryonic stem cells are strikingly sensitive to temporary MYC blockade. [Cell Rep] Full Article | Graphical Abstract H3K36 Histone Methyltransferase Setd2 Is Required for Murine Embryonic Stem Cell Differentiation toward Endoderm Researchers show that Setd2 mainly regulates differentiation of murine embryonic stem cells toward primitive endoderm. [Cell Rep] Full Article | Graphical Abstract Nrf2, a Regulator of the Proteasome, Controls Self-Renewal and Pluripotency in Human Embryonic Stem Cells Investigators show that Nrf2 acts as a key pluripotency gene and a regulator of proteasome activity in human embryonic stem cells. [Stem Cells] Abstract Xist Repression Shows Time-Dependent Effects on the Reprogramming of Female Somatic Cells to Induced Pluripotent Stem Cells Researchers demonstrate that the downregulation of Xist by an isopropyl β-d-1-thiogalactopyranoside-inducible short hairpin RNA system can greatly impair the mesenchymal-to-epithelial transition in the early phase of induced pluripotent stem cells (iPSC) induction but can significantly promote the transition of pre-iPSCs to iPSCs in the late phase. [Stem Cells] Abstract Human Germ Cell Formation in Xenotransplants of Induced Pluripotent Stem Cells Carrying X Chromosome Aneuploidies Scientists derived induced pluripotent stem cells from Turner syndrome and control individuals and examined germ cell development as a function of X chromosome composition. [Sci Rep] Full Article PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells Because germline regulators such as OCT4, NANOG, and SOX2 are known to be potent factors that reprogram differentiated somatic cells into induced pluripotent stem cells (iPSCs), researchers investigated whether the PIWI protein family is involved in iPSC production. [PLoS One] Full Article Transcriptomic Characterization of C57BL/6 Mouse Embryonic Stem Cell Differentiation and Its Modulation by Developmental Toxicants The authors characterized gene expression changes during mouse embryonic stem cell differentiation and their modulation by developmental toxicants. [PLoS One] Full Article Combinatorial Modulation of Signaling Pathways Reveals Cell-Type-Specific Requirements for Highly Efficient and Synchronous iPSC Reprogramming The differentiated state of somatic cells provides barriers for the derivation of induced pluripotent stem cells (iPSCs). To address why some cell types reprogram more readily than others, scientists studied the effect of combined modulation of cellular signaling pathways. [Stem Cell Reports] Full Article | Press Release Mll2 Controls Cardiac Lineage Differentiation of Mouse Embryonic Stem Cells by Promoting H3K4me3 Deposition at Cardiac-Specific Gene Investigators show that Mll2, a trithorax member, is required for proliferation but is dispensable for maintaining the pluripotency of mouse embryonic stem cells (ESCs). Differentiation of ESCs toward mesodermal and endodermal lineages is severely altered and, in particular, the cardiac lineage differentiation of ESCs is completely abolished in the absence of Mll2. [Stem Cell Rep] Abstract |