Predicting the Human Epigenome from DNA Motifs Scientists cataloged the cis elements in embryonic stem cells and four derived lineages and found numerous motifs that have location preference, such as at the center of H3K27ac or at the edges of H3K4me3 and H3K9me3, which provides mechanistic insight about the shaping of the epigenome. [Nat Methods] Abstract OVOL2 Is a Critical Regulator of ER71 in Generating FLK1+ Cells, Hematopoietic and Endothelial Cells from Embryonic Stem Cells Researchers investigated an altered translation factor in three cell states considered to have reduced general translation: immature Xenopus laevis oocytes, mouse ES cells, and the transition state of proliferating mammalian cells to quiescence upon growth-factor deprivation. [Proc Natl Acad Sci USA] Abstract Control of Embryonic Stem Cell Identity by BRD4-Dependent Transcriptional Elongation of Super-Enhancer-Associated Pluripotency Genes Scientists demonstrate that BRD4, a member of the bromodomain and extraterminal domain family of epigenetic readers, regulates the self-renewal ability and pluripotency of embryonic stem cells. [Cell Rep] Full Article | Graphical Abstract | Press Release Induction of the G2/M Transition Stabilizes Haploid Embryonic Stem Cells Researchers developed a parthenogenetic haploid embryonic stem cell culture condition using a small molecular inhibitor of Wee1 kinase to regulate the cell cycle by accelerating the G2/M phase transition and preventing re-entry into extra G1/S phase. [Development] Abstract DEPTOR Is a Stemness Factor that Regulates Pluripotency of Embryonic Stem Cells Scientists show that DEPTOR plays a key role in maintaining stem cell pluripotency by limiting mTOR activity in undifferentiated embryonic stem cells (ESCs). DEPTOR levels dramatically decrease with differentiation of mouse ESCs and knockdown of DEPTOR is sufficient to promote ESC differentiation. [J Biol Chem] Abstract | Full Article Temporal Analysis of Genome Alterations Induced by Single-Cell Passaging in Human Embryonic Stem Cells Researchers prospectively monitored three human embryonic stem cell lines that were initially derived and cultured on human feeders and passaged mechanically before switching to enzymatic single-cell passaging. [Stem Cells Dev] Abstract Directed Differentiation of Embryonic Stem Cells Using a Bead-Based Combinatorial Screening Method The authors describe three experiments comprising stepwise exposure of mouse or human embryonic cells to 10,000 combinations of serum-free differentiation media, through which they discovered multiple novel, efficient and robust protocols to generate a number of specific hematopoietic and neural lineages. [PLoS One] Full Article | Press Release Defining Differentially Methylated Regions Specific for the Acquisition of Pluripotency and Maintenance in Human Pluripotent Stem Cells via Microarray Investigators determined the DNA methylation profiles of ten human cell lines, including two embryonic stem cell (ESC) lines, four virally derived induced pluripotent stem cell (iPSC) lines, two episomally derived iPSC lines, and the two parental cell lines from which the iPSCs were derived using Illumina’s Infinium HumanMethylation450 BeadChip. [PLoS One] Full Article Cyclin D1 Acts as a Barrier to Pluripotent Reprogramming by Promoting Neural Progenitor Fate Commitment Investigators identified Cyclin D1 to be capable of transcriptionally upregulating Pax6, which promoted reprogramming cells to commit to a neural progenitor fate rather than a pluripotent cell fate. [FEBS Lett] Abstract Single-Cell Cloning and Expansion of Human Induced Pluripotent Stem Cells by a Microfluidic Culture Device Researchers demonstrate that a newly developed microfluidic device helped to improve culture conditions and establish a clonality-validated human pluripotent stem cell line after tracing its growth at the single-cell level. [Biochem Biophys Res Commun] Abstract |