Combinatorial Analysis of Developmental Cues Efficiently Converts Human Pluripotent Stem Cells into Multiple Neuronal Subtypes Scientists describe a scalable, automated approach to systematically test the combinatorial actions of small molecules for the targeted differentiation of human pluripotent stem cells (hPSCs). Applied to the generation of neuronal subtypes, this analysis revealed an unappreciated role for canonical Wnt signaling in specifying motor neuron diversity from hPSCs and allowed us to define rapid, efficient procedures to generate spinal and cranial motor neurons as well as spinal interneurons and sensory neurons. [Nat Biotechnol] Abstract | Press Release Human ESC-Derived Dopamine Neurons Show Similar Preclinical Efficacy and Potency to Fetal Neurons when Grafted in a Rat Model of Parkinson’s Disease The authors provide a comprehensive preclinical assessment of human embryonic stem cell (hESC)-derived midbrain dopamine neurons in a rat model of Parkinson’s disease. They show long-term survival and functionality using clinically relevant MRI and PET imaging techniques and demonstrate efficacy in restoration of motor function with a potency comparable to that seen with human fetal dopamine neuron [Cell Stem Cell] Full Article | Graphical Abstract | Press Release hPSC-Derived Maturing GABAergic Interneurons Ameliorate Seizures and Abnormal Behavior in Epileptic Mice Scientists demonstrate that human pluripotent stem cells (hPSC)-derived maturing GABAergic interneurons migrate extensively and integrate into dysfunctional circuitry of the epileptic mouse brain. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Transdifferentiation of Human Fibroblasts to Endothelial Cells: Role of Innate Immunity Scientists show that toll-like receptor 3 (TLR3) agonist Poly I:C, combined with exogenous EC growth factors, transdifferentiated human fibroblasts into ECs. Induced ECs significantly improved limb perfusion and neovascularization in the murine ischemic hindlimb. [Circulation] Abstract | Press Release iPSC-Derived Dopamine Neurons Reveal Differences between Monozygotic Twins Discordant for Parkinson’s Disease Investigators applied induced pluripotent stem cell (iPSC) technology for Parkinson’s disease modeling using twins’ fibroblasts to evaluate and dissect genetic and nongenetic contributions. [Cell Rep] Full Article | Graphical Abstract | Press Release Acute Loss of Cited2 Impairs Nanog Expression and Decreases Self-Renewal of Mouse Embryonic Stem Cells The authors show that Cited2 is important for proliferation, survival and self-renewal of mouse ESC. They position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3 and Klf4 as its direct targets. [Stem Cells] Abstract Induced Pluripotent Stem Cells Restore Function in a Human Cell Loss Model of Open-Angle Glaucoma Using controlled cell loss in ex vivo perfused human outflow pathway organ culture, the authors developed compelling experimental evidence that this level of cell loss compromises intraocular pressure homeostatic function. This function was restored by repopulation of the model with fresh trabecular meshwork cells. They then differentiated induced pluripotent stem cells and used them to repopulate this cell depletion model. [Stem Cells] Abstract | Full Article Reactivation of Inactive X Chromosome and Post-Transcriptional Reprogramming of Xist in Induced Pluripotent Stem Cells Researchers investigated whether the sex of somatic cells affects reprogramming efficiency, differentiation potential, and the post-transcriptional processing of Xist RNA after reprogramming. [J Cell Sci] Abstract Direct Lineage Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells Investigators used transduction of doxycycline-inducible transcription factors to generate stable tripotent neural stem cells (NSCs). The induced NSCs maintained their characteristics in the absence of exogenous factor expression and were transcriptionally, epigenetically, and functionally similar to primary brain-derived NSCs. [Stem Cell Rep] Full Article | Graphical Abstract | Press Release |