The Role of Microscaffold Properties in Controlling the Collagen Assembly in 3D Dermis Equivalent using Modular Tissue Engineering Investigators succeeded in creating 3D viable dermis equivalent tissue, via a bottom-up method, and proved that the final properties, in terms of collagen assembly and organization of the 3D tissue, are tunable and controllable by micro-scaffold properties and degradation rate. [Biomaterials] Abstract Cooperation of Binding Sites at the Hydrophilic Domain of Cell-Surface Sulfatase Sulf1 Allows for Dynamic Interaction of the Enzyme with Its Substrate Heparan Sulfate Sulf1 is a cell-surface sulfatase removing internal 6-O-sulfate groups from heparan sulfate (HS) chains. Thereby it modulates the activity of HS-dependent growth factors. For HS interaction Sulf1 employs a unique hydrophilic domain. Affinity-chromatography, AFM-single-molecule force spectroscopy and immunofluorescence on living cells were used to analyze specificity, kinetics and structural basis of this interaction. [Biochim Biophys Acta] Abstract Evidence for the Regulatory Role of Lipocalin 2 in High-Fat Diet-Induced Adipose Tissue Remodeling in Male Mice The authors investigated the role of Lipocalin 2 (Lcn2) in adipose tissue remodeling during high fat diet-induced obesity. They found that Lcn2 protein is highly abundant selectively in inguinal adipose tissue. [Endocrinology] Abstract In Vitro Mesenchymal Trilineage Differentiation and Extracellular Matrix Production by Adipose and Bone Marrow Derived Adult Equine Multipotent Stromal Cells on a Collagen Scaffold Researchers evaluated the capability of equine multipotent stromal cells from bone marrow and adipose tissue on a type I collagen scaffold to undergo chondrogenic, osteogenic and adipogenic differentiation and form extracellular matrix in vitro. [Stem Cell Rev] Full Article Prediction and Analysis of Higher-Order Coiled-Coils: Insights from Proteins of the Extracellular Matrix, Tenascins and Thrombospondins Scientists evaluated LOGICOIL, a new multi-state predictor for coiled-coil domains, with regard to families of extracellular matrix proteins. [Int J Biochem Cell Biol] Abstract Porous Chitosan Scaffolds with Embedded Hyaluronic Acid/Chitosan/Plasmid-DNA Nanoparticles Encoding TGF-β1 Induce DNA Controlled Release, Transfected Chondrocytes, and Promoted Cell Proliferation In this study, tissue engineering and local therapeutic gene delivery systems are combined with the design of a novel gene-activated matrix embedded with hybrid hyaluronic acid/chitosan/plasmid-DNA nanoparticles encoding transforming growth factor (TGF)-β1. A chitosan scaffold functioned as the three-dimensional carrier for the nanoparticles. [PLoS One] Full Article Uniaxial Cyclic Strain Stimulates Cell Proliferation and Secretion of IL-6 and VEGF of Human Dermal Fibroblasts Seeded on Chitosan Scaffolds Human dermal fibroblasts were inoculated into chitosan sponge scaffolds coated with type I collagen and might be developed as a dermal substitute and/or dressing material. [J Biomed Mater Res A] Abstract |