Substrates with Patterned Extracellular Matrix and Subcellular Stiffness Gradients Reveal Local Biomechanical Responses A substrate fabrication process is developed to pattern both the extracellular matrix and rigidity at sub-cellular spatial resolution. When growing cells on these substrates, it is found that cells respond locally in their cytoskeleton assembly. [Adv Mater] Abstract α SNAP Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells The authors found that siRNA-mediated knockdown of N-ethylmaleimide sensitive factor attachment protein alpha (αSNAP) induced detachment of intestinal epithelial cells, while overexpression of αSNAP increased extracellular matrix adhesion and inhibited cell invasion. [J Biol Chem] Abstract | Full Article The Critical Role of Sestrin 1 in Regulating the Proliferation of Cardiac Fibroblasts With knockdown of Sestrin 1 by siRNA, investigators surveyed the effect of Sestrin 1 on cardiac fibroblast proliferation. Downregulation of Sestrin 1 promotes Ang II-induced proliferation of cardiac fibroblasts, leading to increased DNA synthesis and collagen production. [Arch Biochem Biophys] Abstract Periodically-Perforated Core-Shell Collagen Biomaterials Balance Cell Infiltration, Bioactivity, and Mechanical Properties Scientists used indentation analyses to demonstrate that collagen-glycosaminoglycan (CG) membranes, while less permeable than porous CG scaffolds, show similar permeability to dense materials such as small intestine submucosa. [J Biomed Mater Res A] Abstract Human Collagen-Based Multi-Layer Scaffolds for Tendon-to-Bone Interface Tissue Engineering Researchers developed a human collagen-based multi-layer scaffold mimicking the tendon-to-bone region. The multi-layer scaffold provided a gradual transition of the physical, chemical, mechanical environment and supported the adhesion and proliferation of human fibroblasts, chondrocytes, and osteoblasts towards each corresponding matrix. [J Biomed Mater Res A] Abstract Fibronectin Alters the Rate of Formation and Structure of the Fibrin Matrix Scientists examined the influence of fibronectin on the kinetics of formation, structural characteristics and composition of reconstituted fibrin clots or fibrin matrices. Fibrin matrices were formed by adding thrombin to 1, 2 or 4 mg/ml fibrinogen supplemented with 0-0.4 mg/ml fibronectin. [Biochem Biophys Res Commun] Abstract In Vitro Synthesis of Tensioned Synoviocyte Bioscaffolds for Meniscal Fibrocartilage Tissue Engineering Investigators compared meniscal -like extracellular matrix content of hyperconfluent synoviocyte cell sheets and hyperconfluent synoviocyte sheets which have been tensioned over wire hoops (tensioned synoviocyte bioscaffolds) and cultured for one month. [BMC Vet Res] Full Article Matrix Identity and Tractional Forces Influence Indirect Cardiac Reprogramming The authors identified matrix identity and cell-generated tractional forces as key determinants of the dedifferentiation and differentiation stages during reprogramming. Cell proliferation, matrix mechanics, and matrix microstructure are also important, but play lesser roles. [Sci Rep] Full Article |