Specific Collagen XVIII Isoforms Promote Adipose Tissue Accrual via Mechanisms Determining Adipocyte Number and Affect Fat Deposition Researchers describe a previously unidentified extracellular matrix-directed mechanism contributing to the control of the multistep adipogenic program that determines the number of precursors committing to adipocyte differentiation, the maintenance of the differentiated state, and the physiological consequences of its impairment on ectopic fat deposition. [Proc Natl Acad Sci USA] Abstract Caveolin-1 Is Required for TGF-β-Induced Transactivation of the EGF Receptor Pathway in Hepatocytes through the Activation of the Metalloprotease TACE/ADAM17 Researchers show that immortalized Cav1−/− hepatocytes were more sensitive to the pro-apoptotic effects induced by transforming growth factor-beta (TGF-β), showing a higher activation of caspase-3, higher decrease in cell viability and prolonged increase through time of intracellular reactive oxygen species. [Cell Death Dis] Full Article Ubiquitin-Conjugating Enzyme Complex Uev1A-Ubc13 Promotes Breast Cancer Metastasis through Nuclear Factor-κB Mediated Matrix Metalloproteinase-1 Gene Regulation Scientists investigated roles of different UEV1 splicing variants, and its close homolog MMS2, in promoting tumorigenesis and metastasis in breast cancer cells. They experimentally manipulated the UEV1 and MMS2 levels in MDA-MB-231 breast cancer cells and monitored their effects on cell invasion and migration, as well as tumor formation and metastasis in xenograft mice. [Breast Cancer Res] Abstract | Full Article Localized Micro- and Nano-Scale Remodeling in the Diabetic Aorta Scientists found that the medial aortic layer underwent significant thickening in diabetic animals but without significant changes in collagen or elastin composition (or abundance). Scanning acoustic microscopy demonstrated that such tissue remodeling was associated with a significant decrease in acoustic wave speed in the inter-lamellar spaces of the vessel wall. [Acta Biomater] Full Article Rapid Prototyping Amphiphilic Polymer/Hydroxyapatite Composite Scaffolds with Hydration-Induced Self-Fixation Behavior Researchers demonstrate the feasibility of rapid prototyping biphasic PLA-PEG-PLA (PELA)/hydroxyapatite-PELA scaffolds for potential guided bone regeneration where an osteoconductive scaffold interior encouraging osteointegration and a non-adhesive surface discouraging fibrous tissue encapsulation is desired. [Tissue Eng Part C Methods] Abstract Targeted Injection of a Biocomposite Material Alters Macrophage and Fibroblast Phenotype and Function following Myocardial Infarction: Relation to LV Remodeling The effects of calcium hydroxyapatite injections upon key cell types responsible for the myocardial infarction remodeling process, the macrophage and fibroblast, were examined. [J Pharmacol Exp Ther] Abstract Mechanical Checkpoint for Persistent Cell Polarization in Adhesion-Naive Fibroblasts Using nonadhered round fibroblast cells, scientists show that stiffness sensing through single localized integrin-mediated cues are necessary and sufficient to trigger and direct a shape polarization. [Biophys J] Abstract Processing Large-Diameter Poly(L-Lactic Acid) Microfiber Mesh/Mesenchymal Stromal Cell Constructs via Resin Embedding: An Efficient Histologic Method Scientists performed a complete histologic analysis of constructs based on large diameter poly-L-lactic acid microfibers obtained via dry-wet spinning and rat mesenchymal stromal cells differentiated towards the osteogenic lineage, using acrylic resin embedding. [Biomed Mater] Abstract |