Quantitative High Throughput Screening Using a Primary Human Three-Dimensional Organotypic Culture Predicts In Vivo Efficacy Investigators showed that a multilayered culture containing primary human fibroblasts, mesothelial cells and extracellular matrix can be adapted into a reliable 384- and 1,536-multi-well high throughput screening assay that reproduces the human ovarian cancer metastatic microenvironment. [Nat Commun] Abstract | Press Release Transplantation of Bovine Adrenocortical Cells Encapsulated in Alginate Scientists created a bioartificial adrenal with 3D cell culture conditions by encapsulation of bovine adrenocortical cells (BACs) in alginate. They found that, compared with BACs in monolayer culture, encapsulation in alginate significantly increased the life span of BACs. [Proc Natl Acad Sci USA] Abstract | Full Article Stretching Fibroblasts Remodels Fibronectin and Alters Cancer Cell Migration Researchers investigated the role of mechanical stimuli on human prostatic fibroblasts using a microfluidic platform. Stretched normal tissue-associated fibroblasts exhibited an enhanced capability for directing co-cultured cancer cell migration in a persistent manner. [Sci Rep] Full Article Fibronectin Promotes Cell Proliferation and Invasion through mTOR Signaling Pathway Activation in Gallbladder Cancer Investigators found that fibronectin (FN) was overexpressed in gallbladder cancer (GBC) tissues and was associated with a worse prognosis in GBC patients. In vitro experimental studies indicated that exogenous FN significantly enhanced cell proliferation, invasion and active MMP-9 secretion in human GBC cell lines. [Cancer Lett] Abstract Matrix Metalloproteinase-9 Is Essential for Physiological Beta Cell Function and Islet Vascularization in Adult Mice Researchers investigated the effects of the extracellular matrix-degrading enzyme gelatinase B/matrix metalloproteinase-9 (Mmp-9) on islet function in mice. Islet function of Mmp9-deficient mice and their wild-type littermates was evaluated both in vivo and in vitro. [Am J Pathol] Abstract | Full Article Substrate Stiffness and Composition Specifically Direct Differentiation of Induced Pluripotent Stem Cells Investigators determined the effect that the stiffness and protein composition of the underlying substrate has on the differentiation of induced-pluripotent stem cells, and the potential synergy with specific soluble cues. [Tissue Eng Part A] Abstract Maintenance of HL-1 Cardiomyocyte Functional Activity in PEGylated Fibrin Gels Scientists cultured HL-1 cardiomyocytes either on top of plated polyethylene glycol (PEG) fibrin or embedded in 3D gels and evaluated cardiomyocyte function by assessing the expression of cardiomyocyte specific markers, sarcomeric α-actin and connexin 43, as well as contractile activity. [Biotechnol Bioeng] Abstract Profilin1 Regulates Invadopodium Maturation in Human Breast Cancer Cells Researchers showed that depletion of profilin1 leads to an increase in the number of mature invadopodia and these assemble and disassemble more rapidly than in control cells. They found that loss of profilin1 expression increased the levels of PI(3,4)P2 at the invadopodium and as a result, enhanced recruitment of the interacting adaptor Tks5. [Eur J Cell Biol] Abstract | Press Release Photo-Crosslinkable Biopolymers Targeting Stem Cell Adhesion and Proliferation: The Case Study of Gelatin and Starch-Based IPNs The authors focused on the development of biomaterials that support the adhesion and the proliferation of adipose-tissue derived stem cells. An in vitro cellular assay revealed that the hydrogels developed are biocompatible and supported cell adhesion of adipose-tissue derived mesenchymal stem cells. [J Mater Sci-Mater M] Abstract Substrate Stiffness Affects Human Keratinocyte Colony Formation Researchers investigated the role of substrate elasticity (stiffness) on keratinocyte colony formation in vitro during the process of nascent epithelial sheet formation as triggered by the calcium switch model of keratinocyte culture. [Cell Mol Bioeng] Abstract Acellular Lung Scaffolds Direct Differentiation of Endoderm to Functional Airway Epithelial Cells: Requirement of Matrix-Bound HS Proteoglycans Scientists examined the role of the lung extracellular matrix in differentiation of pluripotent cells in vitro and demonstrated the robust inductive capacity of the native lung matrix alone. [Stem Cell Reports] Full Article | Graphical Abstract |