3D Scaffolds with Different Stiffness but the Same Microstructure for Bone Tissue Engineering Investigators developed novel three-dimensional (3D) scaffolds with different degrees of stiffness but the same 3D microstructure that was maintained by using decellularized cancellous bone. [ACS Appl Mater Interfaces] Abstract | Graphical Abstract Promotion of Periostin Expression Contributes to the Migration of Schwann Cells Scientists performed comparative gene expression analysis of dorsal root ganglia (DRG) explant cultures from erbB3 deficient and wild type mice to identify genes that are involved in Schwann cell development and migration. The extracellular matrix gene periostin was found to exhibit the most prominent down regulation in erbB3 deficient DRG. [J Cell Sci] Abstract Vinculin Is Required for Cell Polarization, Migration, and Extracellular Matrix Remodeling in 3D Collagen To address the role of vinculin in 3D cell migration, investigators analyzed the morphodynamics, migration, and extracellular matrix remodeling of primary murine embryonic fibroblasts with cre/loxP-mediated vinculin gene disruption in 3D collagen I cultures. [FASEB J] Abstract Tissue-Specific Effects of Esophageal Extracellular Matrix Scientists characterized the in vitro cellular response and in vivo host response to a homologous esophageal mucosal extracellular matrix (ECM) vs. non-homologous ECMs derived from small intestinal submucosa and urinary bladder. [Tissue Eng Part A] Abstract Reduced FoxO3a Expression Causes Low Autophagy in Idiopathic Pulmonary Fibrosis Fibroblasts on Collagen Matrix Researchers investigated whether reduced FoxO3a expression causes abnormally low autophagy in idiopathic pulmonary fibrosis (IPF) fibroblasts on collagen. They found that FoxO3a mRNA and protein levels are low in IPF fibroblasts, which subsequently suppresses the autophagosomal marker, LC3-B expression on collagen matrix. [Am J Physiol Lung Cell Mol Physiol] Abstract Extracellular Matrix Stiffness Modulates VEGF Calcium Signaling in Endothelial Cells: Individual Cell and Population Analysis The authors examined endothelial cell calcium signaling in response to VEGF as a function of extracellular matrix stiffness. They developed a new analytical tool to analyze both population based and individual cell responses. Endothelial cells on soft substrates, 4 kPa, were the most responsive to VEGF, whereas cells on the 125 kPa substrates exhibited an attenuated response. [Integr Biol] Abstract Nanometer-Sized Extracellular Matrix Coating on Polymer-Based Scaffold for Tissue Engineering Applications Researchers report a novel approach for layer-by-layer fabrication of nanometer-size fibronectin and gelatin layers on electrospun fibrous poly(carbonate urethane)urea scaffolds. [J Biomed Mater Res A] Abstract Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a Investigators demonstrated that elevated concentration of Hcy induced the expression of collagen type I in cultured human liver cells as well as in liver tissue of hyperhomocysteinemia mice. [PLoS One] Full Article |