Chitosan Microspheres with Extracellular Matrix-Mimicking Nanofibrous Structure as Cell-Carrier Building Blocks for Bottom-Up Cartilage Tissue Engineering Investigators report the fabrication of chitosan microspheres with extracellular matrix-mimicking nanofibrous structure for the first time based on a physical gelatin process. [Nanoscale] Abstract Arsenic Promotes NF-κB-Mediated Fibroblast Dysfunction and Matrix Remodeling to Impair Muscle Stem Cell Function Scientists tested the novel hypothesis that arsenic drives a maladaptive fibroblast phenotype to promote pathogenic myomatrix remodeling and compromise the muscle stem cell niche. [Stem Cells] Abstract | Press Release Osteogenesis of Peripheral Blood Mesenchymal Stem Cells in Self Assembling Peptide Nanofiber for Healing Critical Size Calvarial Bony Defect Peripheral blood mesenchymal stem cells (PBMSCs) were seeded into a nanofiber scaffold of self-assembling peptide (SAP) and cultured in osteogenic medium. The results indicated SAP can serve as a promising scaffold for PBMSCs survival and osteogenic differentiation in 3D conditions. [Sci Rep] Full Article Human Proximal Tubule Epithelial Cells Cultured on Hollow Fibers: Living Membranes That Actively Transport Organic Cations Unique human conditionally immortalized proximal tubule epithelial cell monolayers were cultured on biofunctionalized MicroPES (polyethersulfone) hollow fiber membranes and functionally tested using microfluidics. [Sci Rep] Full Article Transplantation of Three-Dimensional Artificial Human Vascular Tissues Fabricated Using an Extracellular Matrix Nanofilm-Based Cell-Accumulation Technique Scientists established a novel three-dimensional tissue-constructing technique, referred to as the ‘cell-accumulation method’, which is based on the self-assembly of cultured human cells. In this technique, cells are coated with fibronectin and gelatin to construct extracellular matrix nanofilms and cultured to form multi-layers in vitro. [J Tissue Eng Regen Med] Abstract Cardiac Derived Extracellular Matrix Enhances Cardiogenic Properties of Human Cardiac Progenitor Cells Researchers investigated the influence of the myocardial matrix hydrogel on Sca-1-like human fetal and adult cardiac progenitor cells when encapsulated in 3D in vitro. They also evaluated the use of the myocardial matrix as hydrogel for in vivo cell transplantation and demonstrated that the gelation properties of the hydrogel are not influenced by the cells. [Cell Transplant] Abstract Perlecan Diversely Regulates the Migration and Proliferation of Distinct Cell Types In Vitro The authors cultured various cell types, which were derived from epithelial/endothelial, connective and muscle tissues, in the presence of either antiserum against perlecan or exogenous perlecan, and examined the effects of perlecan on cell migration and proliferation. [Cells Tissues Organs] Abstract Extracellular Matrix Remodeling in Wound Healing of Critical Size Defects in the Mitral Valve Leaflet The synthesis and turnover of valvular extracellular matrix due to healing of a critical sized wound was investigated. Acute valve injury elicited distinct, heterogeneous alterations in valvular matrix composition and structure, resulting in partial wound closure. [Heart Vessels] Abstract |