MT1-MMP Sheds LYVE-1 on Lymphatic Endothelial Cells and Suppresses VEGF-C Production to Inhibit Lymphangiogenesis The authors report that membrane type 1-matrix metalloproteinase (MT1-MMP) is an endogenous suppressor of lymphatic vessel growth. MT1-MMP-deficient mice exhibit spontaneous corneal lymphangiogenesis without concomitant changes in angiogenesis. [Nat Commun] Full Article Single-Cell Differences in Matrix Gene Expression Do Not Predict Matrix Deposition Researchers showed that both chondrocytes and chondrogenically induced mesenchymal stem cells exhibit substantial mRNA expression heterogeneity. Surprisingly, this variability does not correlate with cell-to-cell differences in cartilage-like matrix production. [Nat Commun] Full Article Oncogenic Ras Differentially Regulates Metabolism and Anoikis in Extracellular Matrix-Detached Cells Scientists discovered that oncogenic Ras facilitates the survival of extracellular matrix-detached cancer cells by using distinct effector pathways to regulate metabolism and block anoikis. [Cell Death Differ] Abstract SPARC Promotes Cell Invasion In Vivo by Decreasing Type IV Collagen Levels in the Basement Membrane Using the model of anchor cell invasion through the basement membrane (BM) extracellular matrix in Caenorhabditis elegans, researchers found that secreted protein acidic and rich in cysteine (SPARC) overexpression is highly pro-invasive and rescues BM transmigration in mutants with defects in diverse aspects of invasion, including cell polarity, invadopodia formation, and matrix metalloproteinase expression. [PLoS Genet] Full Article Three-Dimensional Characterization of Mechanical Interactions between Endothelial Cells and Extracellular Matrix during Angiogenic Sprouting Researchers studied the three-dimensional cell-extracellular matrix interactions of endothelial cells that form multicellular structures called sprouts. They analyzed data collected in-situ from angiogenic sprouting experiments and identified the differentiated interaction behavior exhibited by the tip and stalk cells. [Sci Rep] Full Article Tumor-Induced Remote ECM Network Orientation Steers Angiogenesis Investigators used automated sequential microprinting of tumor and endothelial cells in extracellular matrix (ECM) scaffolds to study its mechanical aspects. Quantitative reflection microscopy shows that tumor spheroids induce radial orientation of the surrounding collagen fiber network up to a distance of five times their radius. [Sci Rep] Full Article Collagen VI-NG2 Axis in Human Tendon Fibroblasts under Conditions Mimicking Injury Response Scientists investigated the molecular basis of the interaction between collagen VI and the cell membrane both in tissue sections and fibroblast cultures of human tendon, and analyzed the deposition of collagen VI during migration and myofibroblast trans-differentiation, two crucial events for tendon repair. [Matrix Biol] Full Article BMP7 Enhances the Effect of BMSCs on Extracellular Matrix Remodeling in a Rabbit Model of Intervertebral Disc Degeneration The authors investigated the efficacy and feasibility of stem cell therapy using bone morphogenetic protein (BMP)-7-transduced bone marrow mesenchymal stem cells (BMSCs) to promote extracellular matrix remodeling of degenerated intervertebral discs. [FEBS J] Abstract Fluid-Induced, Shear Stress-Regulated Extracellular Matrix and Matrix Metalloproteinase Genes Expression on Human Annulus Fibrosus Cells Researchers investigated the expression of the extracellular matrix and matrix metalloproteinase genes in human annulus fibrosus cells following fluid-induced shear stress in a custom-fabricated bio-microfluidic device. [Stem Cell Res Ther] Full Article |