Hematopoiesis News Volume 5.03 | Jan 28 2014

High c-Kit Expression Identifies Hematopoietic Stem Cells with Impaired Self-Renewal and Megakaryocytic Bias
Investigators demonstrate that even the most rigorously defined hematopoietic stem cells (HSCs) can be separated into functionally distinct subsets based on c-Kit activity. Functional and transcriptome studies showed HSCs with low levels of surface c-Kit expression (c-Kit^lo) and signaling exhibit enhanced self-renewal and long-term reconstitution potential compared with c-Kit^hi HSCs. [J Exp Med] Abstract

Clonal Expansion Capacity Defines Two Consecutive Developmental Stages of Long-Term Hematopoietic Stem Cells
By analyzing the cellular output after transplantation of stem cells differing in surface expression levels of the Kit receptor, scientists showed that long-term hematopoietic stem cells can be systematically subdivided into two subtypes with distinct reconstitution behavior. [J Exp Med] Abstract

Loss of p15Ink4b Accelerates Development of Myeloid Neoplasms in Nup98-HoxD13 Transgenic Mice
Researchers report that loss of p15Ink4b collaborates with Nup98-HoxD13 transgene in the development of predominantly myeloid neoplasms, namely acute myeloid leukemia, myeloproliferative disease and myelodysplastic syndromes. [Stem Cells] Abstract

Hematopoietic Stem/Progenitor Cells Express Myoglobin and Neuroglobin: Adaptation to Hypoxia or Prevention from Oxidative Stress?
Investigators provide for the first time novel functional and molecular evidences that human hematopoietic stem/progenitor cells express myoglobin at level comparable with that of a muscle-derived cell line. [Stem Cells] Abstract

Posttransplantation Bone Marrow Assessment by Quantifying Hematopoietic Cell-Derived mRNAs in Plasma Exosomes/Microvesicles
Researchers hypothesized that hematopoietic precursor cells might release exosomes and microvesicles (EMV) in bone marrow, and the resulting EMV would penetrate into peripheral blood. [Clin Chem] Abstract

Gene and miRNA Expression Profiles of Hematopoietic Progenitor Cells Vary Depending on Their Origin
Hematopoietic progenitors cells from G-CSF mobilized peripheral blood, bone marrow, or umbilical cord blood have different biological properties and different kinetic of engraftment post-transplantation, which might be at least in part explained by a different gene and miRNA expression pattern. In order to assess differences in gene and miRNA expression the authors analyzed whole genome expression profile as well as the expression of 384 miRNAs in CD34+ cells isolated from 18 healthy individuals. [Biol Blood Marrow Transplant] Abstract

Quantitative Phenotyping-Based In Vivo Chemical Screening in a Zebrafish Model of Leukemia Stem Cell Xenotransplantation
Scientists developed a novel phenotype-based in vivo screening method using leukemia stem cells xenotransplanted into zebrafish. Aldehyde dehydrogenase-positive cells were purified from chronic myelogenous leukemia K562 cells tagged with a fluorescent protein (Kusabira-orange) and then implanted in young zebrafish at 48 hours post-fertilization. [PLoS One] Full Article

Flt3 Signaling Regulates the Proliferation, Survival, and Maintenance of Multipotent Hematopoietic Progenitors that Generate B Cell Precursors
To further define the role of Flt3 signaling in lymphoid progenitor survival, mice deficient in Flt3 ligand that also expressed a Bcl2 transgene were generated. [Exp Hematol] Abstract

CLINICAL RESEARCH

HLA-Haploidentical T-Cell Depleted Allogeneic Hematopoietic Stem Cell Transplantation in Children with Fanconi Anemia
Researchers report the outcome of 12 consecutive pediatric patients with Fanconi anemia, who had neither an HLA-identical sibling nor an HLA-matched unrelated donor, and who were given T-cell depleted, CD34⁺ positively selected cells from an haploidentical related donor, after a reduced intensity, fludarabine-based conditioning regimen. [Biol Bone Marrow Transplant] Abstract

Health-Related Quality of Life of Patients with Newly Diagnosed Chronic Myeloid Leukemia Treated with Allogeneic Hematopoietic SCT versus Imatinib
To evaluate and compare the health-related quality of life of patients with newly diagnosed CML in the first chronic phase receiving HLA-identical sibling donor hematopoietic SCT or imatinib, a cross-sectional study that was part of a prospective cohort study at the Institute of Hematology, Peking University was performed. [Bone Marrow Transplant] Abstract

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Minister Sherlock Opens Ireland’s First Human Use Stem Cell Manufacturing Facility at NUI Galway
The Centre for Cell Manufacturing Ireland at NUI Galway is the first ever facility on the island of Ireland to receive a license from the Irish Medicines Board to manufacture culture-expanded stem cells for human use. [National University of Ireland, Galway] Press Release

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NEW Postdoctoral Position – Hematopoietic Stem Cell Physiology and Bone Marrow Failure (University of Michigan)

NEW Research Associate – Normal & Leukemic Stem Cells (King’s College London)

Postdoctoral Fellow – Morphogenetic Signaling Pathways in Development and Disease (University of Rochester Medical Center)

Scientific Affairs Coordinator (American Society of Hematology)

Postdoctoral Position – Erythroid and Myeloid Differentiation of Hematopoietic Stem Cells (Imagine Institute)

Postdoctoral Associate – Hematopoietic Differentiation (Northwestern University Feinberg School of Medicine)

Postdoctoral Fellow – Mammalian Hematopoietic and Erythroid Development (Mount Sinai Medical Center)

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