Hematopoiesis News Volume 5.15 | Apr 22 2014

CD166 Regulates Human and Murine Hematopoietic Stem Cells and the Hematopoietic Niche
Scientists demonstrated that CD166 is a functional hematopoietic stem cell marker that identifies both murine and human long-term repopulating cells. Both murine LSKCD48-CD166+CD150+ and LSKCD48-CD166+CD150+CD9+ cells as well as human Lin-CD34+CD38-CD49f+CD166+ cells sustained significantly higher levels of chimerism in primary and secondary recipients than CD166- cells. [Blood] Abstract

Depletion of Sf3b1 Impairs Proliferative Capacity of Hematopoietic Stem Cells but Is Not Sufficient to Induce Myelodysplasia
Investigators analyzed the function of pre-mRNA splicing factor Sf3b1 in hematopoiesis. Sf3b1^+/- mice maintained almost normal hematopoiesis and did not develop hematological malignancies during a long observation period. [Blood] Abstract

EDAG Positively Regulates Erythroid Differentiation and Modifies GATA1 Acetylation through Recruiting p300
Researchers found that EDAG is highly expressed in CMPs and MEPs and upregulated during the erythroid differentiation of CD34⁺ cells following erythropoietin treatment. [Stem Cells] Abstract

Evi1 Defines Leukemia-Initiating Capacity and Tyrosine Kinase Inhibitor Resistance in Chronic Myeloid Leukemia
Ecotropic viral integration site 1 (Evi1)-internal ribosomal entry site-green fluorescent protein allele models of chronic myeloid leukemia in chronic phase (CML-CP), by retroviral overexpression of BCR-ABL and by crossing BCR-ABL transgenic mice, revealed that Evi1 is predominantly enriched in the stem cell fraction and associated with an enhanced proliferative as well as a leukemia-initiating capacity and that Evi1-high CML-CP cells exhibit resistance to tyrosine kinase inhibitors. [Oncogene] Full Article

Targeted shRNA Screening Identified Critical Roles of Pleckstrin-2 in Erythropoiesis
Using a mouse fetal liver erythroblast culture system and a targeted shRNA functional screening strategy, investigators identified a critical role of pleckstrin-2 in actin dynamics and protection of early stage terminal erythroblasts from oxidative damage. [Haematologica] Abstract | Full Article

IL-3 and CSF-1 Interact to Promote Generation of CD11c⁺ IL-10-Producing Macrophages
Researchers uncovered an instructive role of CSF-1 in interleukin-3 (IL-3)-mediated hematopoiesis. CSF-1 significantly promoted IL-3-driven CD11c⁺ cell expansion and dampened basophil and mast cell generation from C57BL/6 bone marrow. [PLoS One] Full Article

Proteinase-Activated Receptor 1 (PAR1) Regulates Leukemic Stem Cell Functions
Investigators analyzed expression and function of PAR1 in primary hematopoietic cells and their leukemic counterparts. Acute myeloid leukemia patients’ blast cells expressed much lower levels of PAR1 mRNA and protein than CD34⁺ progenitor cells. [PLoS One] Full Article

CLINICAL RESEARCH

Validation and Refinement of the Disease Risk Index for Allogeneic Stem Cell Transplantation: A Study from the CIBMTR
Because the outcome of allogeneic hematopoietic cell transplantation (HCT) is predominantly influenced by disease type and status, it is essential to be able to stratify patients undergoing HCT by disease risk. The Disease Risk Index was developed for this purpose. Investigators analyzed 13,131 patients reported to the CIBMTR who underwent HCT between. [Blood] Abstract

Outcomes of Matched Sibling Donor Hematopoietic Stem Cell Transplantation for Severe Sickle Cell Disease with Myeloablative Conditioning and Intermediate-Dose of Rabbit Anti-Thymocyte Globulin
Investigators postulated that an intermediate dose of rabbit anti-thymocyte (r-ATG) globulin would be effective in preventing both rejection and graft versus host disease. Fifteen patients, median age 5 (range 1.5-18) years, underwent matched sibling donor hematopoietic stem cell transplantation using busulfan, cyclophosphamide and r-ATG. [Pediatr Blood Cancer] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.

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First Volunteers to Receive Blood Cultured from Stem Cells in 2016
Red blood cells cultured in a laboratory will be trialed in human volunteers for the first time within the next three years, as part of a long-term research program funded by the Wellcome Trust. The £5 million Strategic Award was granted to a consortium led by the Scottish National Blood Transfusion Service, and will follow on from previous research that proved that red blood cells could be generated from stem cells. [Wellcome Trust]
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Postdoctoral Position – Development of Novel Treatment for Mixed Lineage Leukemia (Oslo University Hospital)

Postdoctoral Researcher – Regulation of Lymphoid Development (Lund University)

MSc & PhD Positions – Cell Biology, Neurobiology, Neuroimmunology, Cancer Biology, Computational Biology (Hanyang University)

Research Associate – Hematology/Oncology (University of Chicago)

Postdoctoral Fellow – Hematopoietic Stem Cell and Leukemia Research (City of Hope)

Postdoctoral Position – Immunotherapies for Cancer Based on Genetic Engineered T Lymphocytes and/or Hematopoietic Stem Cells (Roswell Park Cancer Institute)

Postdoctoral Fellow – Mammalian Hematopoietic and Erythroid Development (The Icahn School of Medicine at Mount Sinai)

Director of GMP/GLP Quality Operations (University of Pennsylvania, Perelman School of Medicine)

Postdoctoral Position – Human Stem Cell Biology and Hematopoiesis (Johns Hopkins University School of Medicine)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Research Technologist – Particle Chemistry (STEMCELL Technologies Inc.)

Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

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