Niche Displacement of Human Leukemic Stem Cells Uniquely Allows Their Competitive Replacement with Healthy HSPCs Scientists provide functional evidence that the spatial localization of phenotypically primitive human acute myeloid leukemia cells is restricted to niche elements shared with their normal counterparts, and that their intrinsic ability to initiate and retain occupancy of these niches can be rivaled by healthy hematopoietic stem and progenitor cells (HSPCs). [J Exp Med] Abstract Transcription Factor EKLF (KLF1) Recruitment of the Histone Chaperone HIRA Is Essential for β-Globin Gene Expression Researchers found that a novel interaction between erythroid Krüppel-like factor (EKLF) and the histone cell cycle regulation defective homolog A (HIRA) histone chaperone accounts for these effects. HIRA is not only critical for β-globin expression but is also required for activation of the erythropoietic regulators EKLF and GATA binding protein 1. [Proc Natl Acad Sci USA] Abstract The Runx-PU.1 Pathway Preserves Normal and AML/ETO9a Leukemic Stem Cells Scientists show that Runx factors are essential in hematopoietic stem cells (HSCs) by driving the expression of the hematopoietic transcription factor PU.1. Mechanistically, using a knock-in mouse model in which all three Runx binding sites in the -14kb enhancer of PU.1 are disrupted, they observed failure to form chromosomal interactions between the PU.1 enhancer and its proximal promoter. [Blood] Abstract HOXA9 Promotes Hematopoietic Commitment of Human Embryonic Stem Cells Investigators show that during hematopoietic differentiation of human embryonic stem cells, HOXA9 expression parallels hematopoietic development but is restricted to the hemogenic precursors (HEP), and diminishes as HEPs differentiate into blood cells. [Blood] Abstract CITED2-Mediated Human Hematopoietic Stem Cell Maintenance Is Critical for Acute Myeloid Leukemia Since the transcriptional co-activator CITED2 can be overexpressed in acute myeloid leukemia (AML) cells, investigators analyzed the consequences of high CITED2 expression in normal and AML cells. [Leukemia] Abstract FHL2 Regulates Hematopoietic Stem Cell Functions under Stress Conditions The authors identified FHL2 as a critical regulator of hematopoietic stem cells (HSCs) that is essential for maintaining HSC self-renewal under regenerative stress. They found that Fhl2 loss has limited effects on hematopoiesis under homeostatic conditions. [Leukemia] Abstract Sheep CD34+ Amniotic Fluid Cells Have Hematopoietic Potential and Engraft after Autologous In Utero Transplantation Scientists studied the functional hematopoietic potential of transduced GFP+ sheep amniotic fluid stem cells, before and after autologous in utero stem cell transplantation. [Stem Cells] Abstract Lysosomal Cross-Correction by Hematopoietic Stem Cell-Derived Macrophages via Tunneling Nanotubes Despite controversies on the potential of hematopoietic stem cells (HSCs) to promote tissue repair, the authors previously showed that HSC transplantation could correct cystinosis, a multi-systemic lysosomal storage disease, caused by a defective lysosomal membrane cystine transporter, cystinosin. Addressing the cellular mechanisms, they report vesicular cross-correction after HSC differentiation into macrophages. [Stem Cells] Abstract CD133-Targeted Gene Transfer into Long-Term Repopulating Hematopoietic Stem Cells Scientists show that the lentiviral vector CD133-LV, which uses a surface marker on human primitive hematopoietic stem cells as entry receptor, transfers genes preferentially into cells with high engraftment capability. [Mol Ther] Abstract CLINICAL RESEARCH The Impact of Total Body Irradiation on the Outcome of Patients with Follicular Lymphoma Treated with Autologous Stem Cell Transplantation in the Modern Era. A Retrospective Study of the EBMT Lymphoma Working Party Researchers investigated the impact of the high-dose regimen on the outcome of patients with follicular lymphoma having had autologous stem cell transplantation in a recent time period. [Ann Oncol] Abstract Phase II Study of Targeted Therapy with Temozolomide in Acute Myeloid Leukemia and High-Risk Myelodysplastic Syndrome Patients Pre-Screened for Low O6-Methylguanine DNA Methyltransferase Expression Resistance to temozolomide is largely mediated by the DNA repair enzyme O6-methylguanine DNA methyltransferase. Investigators conducted a prospective multicenter study of patients with previously untreated acute myeloid leukemia or high-risk myelodysplastic syndrome who were not candidates for intensive therapy. [Br J Haematol] Abstract |