Hematopoiesis News Volume 6.39 | Oct 6 2015

Single Cell RNA-Seq Reveals Changes in Cell Cycle and Differentiation Programs upon Aging of Hematopoietic Stem Cells
Investigators used single cell RNA-seq to dissect variability in hematopoietic stem and progenitor cell populations from young and old mice from two strains. They found that cell cycle dominates the variability within each population, and that there is a lower frequency of cells in the G1 phase among old compared to young long-term hematopoietic stem cells, suggesting that they traverse through G1 faster. [Genome Res] Abstract

Epigenetic Control of Apolipoprotein E Expression Mediates Gender-Specific Hematopoietic Regulation
Scientists demonstrated that expression of a truncated DNA methyltransferase 3B isoform DNMT3B7, which has been shown to alter cellular epigenetic patterns, decreases the overall number of hematopoietic stem and progenitor cells, and markedly diminishes blood cell reconstitution within the female hormonal microenvironment. [Stem Cells] Abstract

Vascular Platform to Define Hematopoietic Stem Cell Factors and Enhance Regenerative Hematopoiesis
Investigators examined the capacity of bone marrow endothelial cells (BMECs) to support ex vivo and in vivo hematopoiesis. They demonstrated that AKT1-activated BMECs have a unique transcription factor/cytokine profile that supports functional hematopoietic stem cells in lieu of complex serum and cytokine supplementation. [Stem Cell Rep] Full Article | Graphical Abstract

The Bone Marrow Microenvironment Is Similarly Impaired in Allogeneic Hematopoietic Stem Cell Transplantation Patients with Early and Late Poor Graft Function
Investigators recently reported that bone marrow microenvironment abnormalities may occur in cases of late poor graft function (PGF). To determine whether these abnormalities occur in early PGF, they performed a nested case–control study comparing cellular elements of the bone marrow microenvironment in ten subjects with early PGF, 30 subjects with late PGF and 40 subjects without PGF. [Bone Marrow Transplant] Abstract

Kinetics of Hematopoietic Stem Cells and Supportive Activities of Stromal Cells in a Three-Dimensional Bone Marrow Culture System
The authors analyzed the kinetics of hematopoietic cells, and more than 50% of hematopoietic progenitor cells, including CFU-Mix, CFU-GM and BFU-E in three-dimensional culture were in a resting (non-S) phase. They also examined the hematopoietic supportive ability of stromal cells by measuring the expression of various mRNAs relevant to hematopoietic regulation. [Growth Factors] Abstract

CLINICAL RESEARCH

Long-Term Survival Outcomes of Reduced-Intensity Allogeneic or Autologous Transplantation in Relapsed Grade 3 Follicular Lymphoma
Researchers compared the long-term outcomes after allogeneic (allo-) vs autologous hematopoietic cell transplantation (auto-HCT) in the rituximab era. A total of 197 patients undergoing first reduced-intensity conditioning allo-HCT or first auto-HCT during 2000–2012 were included. [Bone Marrow Transplant] Abstract

A Randomized Controlled Trial of Cyclosporine and Tacrolimus with Strict Control of Blood Concentrations after Unrelated Bone Marrow Transplantation
Investigators performed a randomized controlled trial to compare cyclosporine and tacrolimus with target blood concentrations of 500 and 15 ng/ml, respectively, to prevent acute GVHD after unrelated hematopoietic stem cell transplantation. [Bone Marrow Transplant] Abstract

Visit our reviews page to see a complete list of reviews in the Hematopoiesis research field.

Grants Support Baylor Researchers’ Work on Immunotherapy for Leukemia
Two Baylor College of Medicine researchers have received Translational Research Grants from the Leukemia and Lymphoma Society for projects that use immunotherapy approaches to treat leukemia. [Baylor College of Medicine] Press Release

Kiadis Pharma Announces Initiation of a Repeat-Dosing Phase II Clinical Trial with ATIR101™
Kiadis Pharma N.V., a clinical stage biopharmaceutical company developing innovative T-cell immunotherapy treatments for blood cancers and inherited blood disorders, announced that it has obtained regulatory approvals from the national authorities both in Canada and in Belgium to start a further Phase II clinical trial with its lead product ATIR101™. [Kiadis Pharma N.V.] Press Release

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Visit our events page to see a complete list of events in the Hematopoiesis community.

NEW Associate Professor – Leukemia Research (University of Heidelberg)

NEW Postdoctoral Fellow – Leukemia, Stem Cells and Development (Josep Carreras Leukemia Research Institute)

Principal Scientist – Hematopoietic Products (STEMCELL Technologies Inc.)

Postdoctoral Positions – Cancer and Developmental Biology (North Carolina Central University)

Assistant Professor – Stem Cell Biology (University of South Carolina)

Postdoctoral Position – Hematology and Immunology (University of Liege)

Director of Research – Oncology and Hematology (CancerCare Manitoba)

Postdoctoral Fellow – HMGA Chromatin Remodeling Proteins in Cancer and Stem Cell Biology (Johns Hopkins University School of Medicine)

Regulatory Coordinator – Leukemia (Fred Hutchinson Cancer Research Center)

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