Single-Cell RNA Sequencing Reveals Molecular and Functional Platelet Bias of Aged Hematopoietic Stem Cells By analyzing hematopoietic stem cell (HSC) transcriptomes and HSC function at the single-cell level, scientists identified increased molecular platelet priming and functional platelet bias as the predominant age-dependent change to HSCs, including a significant increase in a previously unrecognized class of HSCs that exclusively produce platelets. [Nat Commun] Full Article MIP-1α/CCL3-Expressing Basophil-Lineage Cells Drive the Leukemic Hematopoiesis of Chronic Myeloid Leukemia in Mice To delineate the cellular process facilitating CCL3-mediated crosstalk between normal and leukemic hematopoiesis, investigators precisely examined CCL3-expressing cells and their functions in both normal hematopoiesis and chronic myeloid leukemia leukemogenesis. [Blood] Abstract Leukemic Stem Cell Quantification in Newly Diagnosed Chronic Myeloid Leukemia Patients Predicts Response to Nilotinib Therapy Researchers identified Philadelphia chromosome positive CD34+CD38– bone marrow cells and addressed their response-predictive value in chronic myeloid leukemia patients subjected to nilotinib. [Clin Cancer Res] Abstract MiR144/451 Expression Is Repressed by RUNX1 during Megakaryopoiesis and Disturbed by RUNX1/ETO Investigators found that the hematopoietic master regulator RUNX1 controls the expression of certain microRNAs, of importance during erythroid/megakaryocytic differentiation. [PLoS Genet] Full Article Abnormal Proplatelet Formation and Emperipolesis in Cultured Human Megakaryocytes from Gray Platelet Syndrome Patients Researchers report the first analysis of cultured megakaryocytes from Gray Platelet Syndrome patients with NBEAL2 mutations. Megakaryocytes cultured from peripheral blood or bone marrow hematopoietic progenitor cells from four patients were used to investigate megakaryopoiesis, megakaryocyte morphology and platelet formation. [Sci Rep] Full Article Long-Term Restoration of Thymidine Phosphorylase Function and Nucleoside Homeostasis Using Hematopoietic Gene Therapy in a Murine Model of MNGIE Scientists showed that ectopic expression of thymidine phosphroylase in the hematopoietic system restores normal nucleoside levels in plasma, as well as in tissues affected in mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) such as small intestine, skeletal muscle, brain and liver. [Hum Gene Ther] Abstract GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia Researchers present evidence that GATA-1 binds to the PU.1 gene and inhibits its expression in human acute myeloid leukemia-erythroleukemias. [PLoS One] Full Article CLINICAL RESEARCH Long-Term Follow-Up of Patients with Acute Myeloid Leukemia Surviving and Free of Disease Recurrence for At Least 2 Years after Autologous Stem Cell Transplantation: A Report from the Acute Leukemia Working Party of the European Society for Blood and Marrow Transplantation Leukemia recurrence is a major cause of treatment failure after autologous stem cell transplantation for acute myeloid leukemia. It usually occurs within the first 2 years after transplantation. Researchers assessed the follow-up of and characterize risk factors for outcome among patients who survived free of disease recurrence after this period. [Cancer] Abstract Somatic, Hematologic Phenotype, Long-Term Outcome and Effect of Hematopoietic Stem Cell Transplantation. An Analysis of 97 Fanconi Anemia Patients from the Italian National Database on Behalf of the Marrow Failure Study Group of the AIEOP (Italian Association of Pediatric Hematology-Oncology) Scientists analyzed 97 Fanconi Anemia patients from a clinic/biological database for genotype, somatic and hematologic phenotype, adverse hematological events, solid tumors and treatment. [Am J Hematol] Abstract |