PRDM16 Suppresses MLL1r Leukemia via Intrinsic Histone Methyltransferase Activity Investigators report that PRDM16 is a histone H3K4 methyltransferase on chromatin. Mutation in the N-terminal PR domain of PRDM16 abolished the intrinsic enzymatic activity of PRDM16. They showed that the methyltransferase activity of PRDM16 is required for specific suppression of MLL fusion protein-induced leukemogenesis both in vitro and in vivo. [Mol Cell] Abstract | Graphical Abstract MiR-22 Has a Potent Anti-Tumor Role with Therapeutic Potential in Acute Myeloid Leukemia Researchers showed that miR-22 is an essential anti-tumor gatekeeper in de novo acute myeloid leukemia where it is significantly downregulated. Forced expression of miR-22 significantly suppressed leukemic cell viability and growth in vitro, and substantially inhibited leukemia development and maintenance in vivo. [Nat Commun] Full Article | Press Release MEK and PI3K-AKT Inhibitors Synergistically Block Activated IL7-Receptor Signaling in T-Cell Acute Lymphoblastic Leukemia Researchers showed that inhibiting both the MEK and PI3K-AKT pathways synergistically prevents the proliferation of BaF3 cells expressing mutant IL7Ra, JAK, and RAS. [Leukemia] Full Article Long-Term In Vitro Maintenance of Clonal Abundance and Leukemia-Initiating Potential in Acute Lymphoblastic Leukemia Investigators showed that Nestin-positive human mesenchymal stem cells support expansion of a range of biologically and clinically distinct patient-derived acute lymphoblastic leukemia samples. [Leukemia] Full Article Erythroleukemia Cells Acquire an Alternative Mitophagy Capability Using CRISPR/Cas9 deletion of the canonical autophagy-essential gene Atg7, scientists found that erythroleukemia K562 cells are armed with two sets of autophagic machinery. [Sci Rep] Full Article Transcriptional, Epigenetic and Retroviral Signatures Identify Regulatory Regions Involved in Hematopoietic Lineage Commitment Investigators defined the transcriptional and epigenetic profile of human hematopoietic stem/progenitor cells and early myeloid and erythroid progenitors by a combination of cap analysis of gene expression, ChIP-seq and Moloney leukemia virus integration site mapping. [Sci Rep] Full Article Endothelial Cell-Selective Adhesion Molecule Expression in Hematopoietic Stem/Progenitor Cells Is Essential for Erythropoiesis Recovery after Bone Marrow Injury To determine the physiological importance of endothelial cell-selective adhesion molecule (ESAM) in erythroid recovery, ESAM-knockout (KO) mice were treated with the anti-cancer drug, 5-fluorouracil (5-FU). ESAM-KO mice experienced severe and prolonged anemia after 5-FU treatment compared to wild-type mice. [PLoS One] Full Article CLINICAL RESEARCH Incidence and Risk Factors of Poor Graft Function after Allogeneic Stem Cell Transplantation for Myelofibrosis The authors retrospectively studied a cohort of 100 patients with primary myelofibrosis (MF) or post-ET/PV MF who received a reduced-intensity hematopoieteic stem cell transplantation. [Bone Marrow Transplant] Abstract Autologous Transplant Remains the Preferred Therapy for Relapsed APL in CR2 Data on patients in CR2 were collected from two large transplant registries. The outcome of patients in CR2 who received only arsenic trioxide (ATO)-based therapy as reinduction was retrospectively compared with those who got an auto-hematopoietic cell transplantation, with or without ATO. [Bone Marrow Transplant] Abstract |