Hematopoiesis News Volume 7.19 | May 17 2016

High Levels of Canonical Wnt Signaling Lead to Loss of Stemness and Increased Differentiation in Hematopoietic Stem Cells
In the blood system, the role of Wnt signaling has been the subject of much debate but there is consensus that high Wnt signals lead to loss of reconstituting capacity. To better understand this phenomenon, the authors have taken advantage of a series of hypomorphic mutant Apc alleles resulting in a broad range of Wnt dosages in hematopoietic stem cells and performed whole-genome gene expression analyses. [Stem Cell Reports] Full Article

Expression of CDKN1C in the Bone Marrow of Patients with Myelodysplastic Syndrome and Secondary Acute Myeloid Leukemia Is Associated with Poor Survival after Conventional Chemotherapy
Investigators tested the hypothesis that proliferative activity of hematopoietic stem cells has impact on survival in newly diagnosed patients with myelodysplastic syndrome and secondary acute myeloid leukemia. [Int J Cancer] Abstract

Calreticulin-Mutant Proteins Induce Megakaryocytic Signaling to Transform Hematopoietic Cells and Undergo Accelerated Degradation and Golgi-Mediated Secretion
Researchers demonstrated the potency of calreticulin mutants to drive expression of megakaryocytic differentiation markers such as NF-E2 and CD41 as well as thrombopoietin receptor. [J Hematol Oncol] Full Article

Repression of Primitive Erythroid Program Is Critical for the Initiation of Multi-Lineage Hematopoiesis in Mouse Development
Investigators found that the earliest committed hematopoietic progenitors highly expressed Gata1, Scl/tal1, and Klf1 genes. Expression of these transcription factors, which is known to form a core erythroid transcriptional network, explained the prompt generation of primitive erythrocytes from these earliest progenitors. [J Cell Physiol] Abstract

MLL1 and MLL1 Fusion Proteins Have Distinct Functions in Regulating Leukemic Transcription Program
The authors demonstrated that mixed lineage leukemia protein-1 (MML1), probably through C-terminal interaction with WDR5, is recruited to regulatory enhancers that are enriched for binding sites of E-twenty-six family transcription factors, whereas MLL-AF9 binds to chromatin regions that have no H3K4me1 enrichment. [Cell Disc] Abstract

CLINICAL RESEARCH

Phase II Study of Intensified Chemotherapy and Allogeneic Hematopoietic Stem Cell Transplantation for Older Patients with Acute Lymphoblastic Leukemia
Scientists determined the efficacy of an intensified, multi-agent approach derived from a Dana-Farber consortium trial in younger adults for patients older than 50 years. [Cancer] Abstract

Busulfan, Melphalan, and Bortezomib versus High Dose Melphalan as a Conditioning Regimen for Autologous Hematopoietic Stem Cell Transplantation in Multiple Myeloma
The authors compared a novel conditioning combination incorporating busulfan, melphalan and bortezomib versus standard melphalan 200 mg/m² in newly diagnosed patients undergoing autologous hematopoietic cell transplantation for multiple myeloma. [Biol Blood Marrow Transplant] Abstract

Filgrastim-Stimulated Bone Marrow Compared to Filgrastim-Mobilized Peripheral Blood in Myeloablative Sibling Allografting for Patients with Hematologic Malignancies: A Randomized Canadian Blood and Marrow Transplant Group Study
Researchers conducted a Phase III, open label, multicenter randomized trial of 230 adults with hematologic malignancies receiving allografts from siblings after myeloablative conditioning to compare filgrastim-mobilized peripheral blood and filgrastim-stimulated marrow. [Biol Blood Marrow Transplant] Abstract

Nonmyeloablative Allogeneic Hematopoietic Cell Transplantation
The authors summarize the encouraging early results seen with the regimens and discuss two hurdles that need to be overcome for achieving even greater success, disease relapse and graft-versus-host disease. [Haematologica] Full Article

DNA Methylation in Hematopoietic Development and Disease
The authors review some of the recent literature regarding the role of DNA methylation in hematopoietic health and disease. [Exp Hematol] Abstract

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HemaCare Signs Distribution Agreement with China-Based Partner
HemaCare Corporation has expanded its global capabilities through a strategic distribution agreement in China with Shanghai-based MT-Biological Science and Technology Co. Ltd. This partnership ensures researchers in China can access HemaCare’s human healthy and disease state cryopreserved hematopoietic cell products for their basic scientific and cell therapy research and development needs in a timely manner and with local region support. [HemaCare Corporation] Press Release

ArcherDX Launches NGS Assays for Hematological Malignancy Profiling
ArcherDX, Inc., announced three new targeted next-generation sequencing assays to capture and identify known and novel gene fusions, point mutations and relative expression levels in myeloid and lymphoid origin malignancies. [ArcherDX, Inc. (Business Wire)] Press Release

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NEW Technical Sales Representative (STEMCELL Technologies Inc.)

NEW Postdoctoral Research Associate – Myeloid Leukemia (Upstate Medical University)

Scientist – Hematology (STEMCELL Technologies Inc.)

PhD Student – Leukemia Biology (Helmholtz Association)

Postdoctoral Fellow – Leukemia Lymphoma (Emory University)

Postdoctoral Scientist – Mouse Hematopoiesis (Lund University)

Postdoctoral Fellow – Hematopoietic Stem Cell Biology and Blood Disorders (Brigham & Women’s Hospital)

Research Technician – Hematopoietic Stem Cell Biology (Fred Hutchinson Cancer Research Center)

Postdoctoral Research Associate – Stem Cell Aging Biology (University of Southern California)

Postdoctoral Fellow – Hematopoietic Stem Cells (University of Pennsylvania)

Postdoctoral Fellow – Hematology/Oncology (Boston Children’s Hospital)

Assistant/Associate/Full Member Faculty – Myeloid Malignancies (Fred Hutchinson Cancer Research Center)

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