The Ro60 Autoantigen Binds Endogenous Retroelements and Regulates Inflammatory Gene Expression Investigators cataloged the Ro60-associated RNAs in human cell lines and found that among other RNAs, Ro60 bound an RNA motif derived from endogenous Alu retroelements. Alu transcripts were induced by type I interferon and stimulated pro-inflammatory cytokine secretion by human peripheral blood cells. [Science] Abstract TCR-Induced Sumoylation of the Kinase PKC-θ Controls T Cell Synapse Organization and T Cell Activation Scientists found that the kinase PKC-θ was sumoylated upon costimulation with antigen or via the T cell antigen receptor (TCR) plus the coreceptor CD28, with Lys325 and Lys506 being the main sumoylation sites. Analysis of primary mouse and human T cells revealed that sumoylation of PKC-θ was essential for T cell activation. [Nat Immunol] Abstract Chimeric Antigen Receptor-Modified T Cells Derived from Defined CD8+ and CD4+ Subsets Confer Superior Antitumor Reactivity In Vivo Investigators analyzed CD19 CAR-expressing effector T-cells derived from different subsets (CD4+/CD8+ naïve, central memory, effector memory). T-cells derived from each of the subsets were efficiently transduced and expanded, but showed clear differences in effector function and proliferation in vitro and in vivo. [Leukemia] Abstract Multiple Sclerosis Associated Genetic Variants of CD226 Impair Regulatory T Cell Function Researchers investigated phenotypical and functional consequences of a genetic variant in the CD226 gene that, among other autoimmune diseases, predisposes to multiple sclerosis. Phenotypically, effector and regulatory CD4+ memory T cells of healthy individuals carrying the predisposing CD226 genetic variant showed, in comparison to carriers of the protective variant, reduced surface expression of CD226 and an impaired induction of CD226 after stimulation. [Brain] Abstract Inhibition of Super-Enhancer Activity in Autoinflammatory Site-Derived T Cells Reduces Disease-Associated Gene Expression With H3K27ac chromatin immunoprecipitation, scientists identified a disease-specific, inflammation-associated, typical enhancer and super-enhancer signature in juvenile idiopathic arthritis patient synovial-fluid-derived CD4+ memory/effector T cells. [Cell Rep] Full Article | Graphical Abstract Immunological Adaptations to Pregnancy in Women with Type 1 Diabetes The systemic immune response was assessed by using flow cytometry to evaluate the number and activational status of subpopulations of lymphocytes, natural killer cells and monocytes in peripheral blood of non-pregnant and pregnant women with and without type 1 diabetes. [Sci Rep] Full Article NK Cells Preferentially Target Tumor Cells with a Cancer Stem Cell Phenotype Researchers demonstrated that activated NK cells are capable of preferentially killing cancer stem cells (CSCs) identified by multiple CSC markers from a wide variety of human cancer cell lines in vitro and dissociated primary cancer specimens ex vivo. [J Immunol] Abstract Signal Strength and Metabolic Requirements Control Cytokine-Induced Th17 Differentiation of Uncommitted Human T Cells Human Th17 cells can be efficiently generated with appropriate cytokines from precommitted precursors, but the requirements of uncommitted T cells are still ill defined. In standard human Th17 cultures, IL-17 production was restricted to CCR6+CD45RA+ T cells, which expressed CD95 and produced IL-17 ex vivo, identifying them as Th17 memory stem cells. [J Immunol] Abstract Bispecific Antibody Releasing-Mesenchymal Stromal Cell Machinery for Retargeting T Cells towards Acute Myeloid Leukemia Blasts Researchers examined the use of mesenchymal stromal cells as autonomous cellular machines for the constant production of a recently described, fully humanized anti-CD33-anti-CD3 bispecific antibody, which is capable of redirecting human T cells against CD33-expressing leukemic cells. [Blood Cancer J] Full Article Subscribe to our sister publications: Immunology of Infectious Disease News & Immune Regulation News! |