Intestinal Cell News Volume 1.07 | May 8 2015

Reducing DNA Methylation Suppresses Colon Carcinogenesis by Inducing Tumor Cell Differentiation
Scientists found that a genome-wide reduction in the DNA methylation levels induced cellular differentiation in association with decreased cell proliferation in Apc^Min/+ mouse colon tumor cells in vivo. [Carcinogenesis] Abstract

An Essential Role of cAMP Response Element-Binding Protein in Epidermal Growth Factor-Mediated Induction of Sodium/Glucose Cotransporter 1 Gene Expression and Intestinal Glucose Uptake
The authors demonstrated that CREB is involved in epidermal growth factor-mediated transcription regulation of the sodium/glucose cotransporter 1 gene in the normal and inflamed intestine. [Int J Biochem Cell Biol] Abstract

Downregulated Long Noncoding RNA BANCR Promotes the Proliferation of Colorectal Cancer Cells via Downregulation of p21 Expression
Researchers showed that BRAF activated non-coding RNA (BANCR) expression was significantly down-regulated in colorectal cancer tissues compared with normal tissues, and overexpression of BANCR suppressed colorectal cancer cell growth in vitro and in vivo. [PLoS One] Full Article

Commensal Streptococcus salivarius Modulates PPARγ Transcriptional Activity in Human Intestinal Epithelial Cells
Researchers used human intestinal epithelial cell lines engineered to monitor peroxisome proliferator-activated receptor (PPARγ) transcriptional activity in response to a wide range of S. salivarius strains. [PLoS One] Full Article

Altered Expression of Caspases-4 and -5 During Inflammatory Bowel Disease and Colorectal Cancer: Diagnostic and Therapeutic Potential
Scientists demonstrated that caspases-4 and -5 are involved in inflammatory bowel disease-associated intestinal inflammation. They also found robust expression of caspases-4 and -5 within intestinal epithelial cells, exclusively within neoplastic tissue, of colorectal tumours. [Clin Exp Immunol] Abstract

Short Hairpin RNA- Mediated Gene Knockdown of FOXM1 Inhibits the Proliferation and Metastasis of Human Colon Cancer Cells through Reversal of Epithelial-to-Mesenchymal Transformation
Investigators investigated FOXM1 protein expression in 87 colorectal cancer tissue specimens, invasive lymph nodes and adjacent paired normal colorectal tissues by immunohistochemical analysis. [J Exp Clin Cancer Res] Abstract

Mitogen-Activated Protein Kinase Phosphatase-1 Prevents Lipopolysaccharide-Induced Apoptosis in Immature Rat Intestinal Epithelial Cells
The authors demonstrated that mitogen-activated protein kinase phosphatase-1 has a central role in preventing inflammation-induced apoptosis in rIEC-6 enterocytes. [Pediatr Res] Abstract

INTESTINAL STEM CELL & ORGANOID RESEARCH

PERK Limits Drosophila Lifespan by Promoting Intestinal Stem Cell Proliferation in Response to ER Stress
The authors showed that the PKR-like ER kinase (PERK) integrates both cell-autonomous and non-autonomous endoplasmic reticulum (ER) stress stimuli to induce intestinal stem cell proliferation. [PLoS Genet] Full Article

Overlapping DNA Methylation Dynamics in Mouse Intestinal Cell Differentiation and Early Stages of Malignant Progression
Genome-scale analysis of DNA methylation together with microarray gene expression have been applied to compare intestinal crypt stem cells, differentiated cells, Apc^Min/+ adenomas and the corresponding non-tumor adjacent tissue, together with small and large intestine samples and the colon cancer cell line CT26. [PLoS One] Full Article

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Generon Received US FDA Clearance to Initiate Phase IIa Study with F-652, a First-in-Class Biologic, to Treat Patients with Acute GvHD
Generon Corporation announced that it received US FDA clearance of the IND for a Phase IIa study, entitled “Recombinant Human Interleukin-22-IgG2-Fc (F-652) in Combination with Systemic Corticosteroids for the Treatment of Newly Diagnosed Grade II-IV Lower Gastrointestinal Acute Graft-versus-Host Disease (GvHD) in Hematopoietic Stem Cell Transplantation Recipients”. [Generon Corporation Ltd.] Press Release

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NEW Postdoctoral Fellow – Principles of Cellular Processes (University of Southern California)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Assistant/Associate Professor – Cardiovascular and Lymphatic Biology (Texas A&M Health Science Center)

Postdoctoral Position – Genomics or Computational Genomics (Norwegian PSC research center)

Postdoctoral Fellow – Stem Cell Homeostasis in Gut Development and Disease (Sickkids Research Institute)

Postdoctoral Position – Physiology (Radboud University Nijmegen Medical Centre)

Postdoctoral Fellow – Transcriptional Mechanisms of Stomach and Intestinal Stem Cells (Sickkids Research Institute)

Postdoctoral Position – Functional Role of Intestinal Macrophages (University of Massachusetts Medical School)

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