INTESTINAL CANCERS & DISEASES Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells Scientists showed that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with segmented filamentous bacteria (SFB) indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. [Cell] Abstract | Graphical Abstract INPP4B Is an Oncogenic Regulator in Human Colon Cancer Investigators showed that silencing of INPP4B blocks activation of Akt and serum- and glucocorticoid-regulated kinase 3, inhibits colon cancer cell proliferation and retards colon cancer xenograft growth. [Oncogene] Full Article Wnt Activated β-Catenin and YAP Proteins Enhance the Expression of Non-Coding RNA Component of RNase MRP in Colon Cancer Cells The authors examined the expression of RMRP and found a significant increase in colorectal and breast cancer patient tissues. They tested whether the oncogenic signaling pathways, Wnt/β-catenin and Hippo/YAP pathways, are relevant to the enhanced expression of RMRP in cancer cells because of the predicted β-catenin/TCF and YAP/TBX5 elements in the upstream regions of the RMRP gene. [Oncotarget] Full Article Clusterin, a Gene Enriched in Intestinal Stem Cells, Is Required for L1-Mediated Colon Cancer Metastasis Researchers identified the clusterin gene that is also enriched in Lgr5+ intestinal stem cells, as a gene induced during L1-mediated colorectal cancer metastasis. [Oncotarget] Full Article Prognostic Significance of Leucine-Rich-Repeat-Containing G-Protein-Coupled Receptor 5, an Intestinal Stem Cell Marker, in Gastric Carcinomas Investigators examined the LGR5 expression in gastric cancer tissues by real time-PCR and RNA in situ hybridization, and analyzed its clinicopathological relevance and prognostic value. The effects of LGR5 on cancer cell proliferation and migration were assessed with an in vitro transfection technique. [Gastric Cancer] Abstract Involvement of Notch-1 in Resistance to Regorafenib in Colon Cancer Cells Scientists generated regorafenib-resistant SW480 cells (Reg-R-SW480 cells) by culturing such cells with increasing concentration of regorafenib. Examination of intracellular signaling found that Akt signaling was activated in Reg-R-SW480 cells but not in wild-type SW480 cells, after regorafenib treatment as measured by Western Blot. [J Cell Physiol] Abstract LPA Induces Colon Cancer Cell Proliferation through a Cooperation between the ROCK and STAT-3 Pathways Researchers investigated the signaling pathways triggered by lysophosphatidic acid (LPA) to regulate the mechanisms involved in the progression of colorectal cancer (CRC). They used three cell line models of CRC, and initially analyzed the expression profile of LPA receptors. [PLoS One] Full Article INTESTINAL STEM CELL & ORGANOID RESEARCH Postnatal Epigenetic Regulation of Intestinal Stem Cells Requires DNA Methylation and Is Guided by the Microbiome Scientists used whole genome bisulfite sequencing, and found that differentiation of mouse colonic intestinal stem cells to intestinal epithelium is not associated with major changes in DNA methylation. They detected extensive dynamic epigenetic changes in intestinal stem cells and their progeny during the suckling period, suggesting postnatal epigenetic development in this stem cell population. [Genome Biol] Full Article Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells when Donor Colon Is Aganglionic The authors hypothesized that tissue-engineered colon (TEC) might contain a diverse population of enteric neuronal subtypes, and that aganglionic TEC can be populated by neurons and glia when supplemented with enteric nervous system progenitor cells in the form of neurospheres. [Tissue Eng Part A] Abstract | Full Article | Press Release |