LABORATORY RESEARCH Unbiased Quantitative Assessment of Her-2 Expression of Circulating Tumor Cells in Patients with Metastatic and Non-Metastatic Breast Cancer Expression of Her-2 on circulating tumor cells was determined by a manual review and an automated algorithm using Her-2- fluorescein isothiocyanate fluorescence of leukocytes to determine the Her-2-expression threshold in each sample. [Ann Oncol] Abstract Suppression of FOXQ1 in Benzyl Isothiocyanate-Mediated Inhibition of Epithelial-Mesenchymal Transition in Human Breast Cancer Cells Loss of E-cadherin expression and induction of mesenchymal markers are biochemical hallmarks of epithelial-to-mesenchymal transition (EMT), a developmental process implicated in progression of cancer to aggressive state. Researchers offer novel insights into the mechanism by which benzyl isothiocyanate inhibits EMT. [Carcinogenesis] Abstract Fucoidan Induces Changes in the Epithelial-Mesenchymal Transition and Decreases Metastasis by Enhancing Ubiquitin-Dependent TGFβ Receptor Degradation in Breast Cancer Using 4T1 and MDA-MB-231 cells, the authors found that fucoidan effectively reverses TGFβ receptor-induced epithelial-mesenchymal transition morphological changes, up-regulates epithelial markers, down-regulates mesenchymal markers, and decreases the expression of transcriptional repressors Snail, Slug, and Twist. [Carcinogenesis] Abstract Neutralization of Prolactin Receptor Function by Monoclonal Antibody LFA102, a Novel Potential Therapeutic for the Treatment of Breast Cancer Scientists describe the biological properties of LFA102, a humanized neutralizing monoclonal antibody directed against the extracellular domain of the prolactin receptor. [Mol Cancer Ther] Abstract The Tyrosine Kinase Inhibitor E-3810 Combined with Paclitaxel Inhibits the Growth of Advanced- Stage Triple-Negative Breast Cancer Xenografts Researchers investigated the activity of E-3810 combined with different cytotoxic agents in a MDA-MB-231 triple-negative breast cancer xenograft model. [Mol Cancer Ther] Abstract Distinct Phospho-Tyrosine Dependent Functions of ShcA Are Required for TGFβ-Induced Breast Cancer Cell Migration, Invasion and Metastasis Through a structure/function approach, investigators showed that the PTB, but not the SH2 domain, of ShcA is required for transforming growth factor beta (TGFβ)-induced migration and invasion of ErbB2-expressing breast cancer cells. [J Biol Chem] Abstract | Full Article Cyclooxygenase-2 Enzyme Induces the Expression of the α-2,3-Sialyltransferase-3 (ST3Gal-I) in Breast Cancer Using the breast cancer cell line T47D, researchers showed that PGE2, one of the final products of the cyclooxygenase-2 pathway, can induce the mRNA expression of the sialyltransferase ST3Gal-I, resulting in increased sialyltransferase activity, demonstrated by a reduction in PNA lectin staining. [J Biol Chem] Abstract The Prognostic Impact of Circulating Tumor Cells in Subtypes of Metastatic Breast Cancer Researchers aimed to assess the impact of circulating tumor cells on overall survival and progression free survival in patients with predefined molecular subgroups of metastatic breast cancer. [Breast Cancer Res Treat] Abstract Estrogen Receptor and Progesterone Receptor Expression in Normal Terminal Duct Lobular Units Surrounding Invasive Breast Cancer Scientists evaluated relationships of breast cancer risk factors and tumor pathology to estrogen receptor and progesterone receptor expression in terminal duct lobular units surrounding breast cancers. [Breast Cancer Res Treat] Abstract CLINICAL RESEARCH Risk of Asynchronous Contralateral Breast Cancer in Noncarriers of BRCA1 and BRCA2 Mutations with a Family History of Breast Cancer: A Report from the Women’s Environmental Cancer and Radiation Epidemiology Study From their population-based case-control study comparing women with contralateral breast cancer (CBC) to women with unilateral breast cancer, researchers selected women who tested negative for BRCA1 and BRCA2 mutations. Rate ratios and 95% CIs were estimated to examine the association between family history of breast cancer and risk of asynchronous CBC. [J Clin Oncol] Abstract Risk of Myelodysplastic Syndrome and Acute Myeloid Leukemia Post Radiation Treatment for Breast Cancer: A Population-Based Study The authors hypothesized that breast cancer radiation treatment is associated with increased risk of myelodysplastic syndromes and acute myeloid leukemia as seen in a previously conducted study. [Breast Cancer Res Treat] Abstract |