LABORATORY RESEARCH Rare Somatic Cells from Human Breast Tissue Exhibit Extensive Lineage Plasticity Scientists identified cell surface markers associated with repression of p16INK4a/cyclin-dependent kinase inhibitor 2A, a critical determinant in the acquisition of a plastic state. These cell surface markers allowed direct isolation of rare cells from healthy human breast tissue that exhibit extensive lineage plasticity. [Proc Natl Acad Sci USA] Abstract | Press Release TNRC9 Downregulates BRCA1 Expression and Promotes Breast Cancer Aggressiveness The breast cancer gene TNRC9 has been associated with disease susceptibility but its function is undetermined. Researchers report that TNRC9 is often amplified and overexpressed in breast cancer, particularly in advanced breast cancer. [Cancer Res] Abstract Inherited Variation in miR-290 Expression Suppresses Breast Cancer Progression by Targeting the Metastasis Susceptibility Gene Arid4b Highly metastatic MMTV-PyMT mice were crossed with AKXD (AKR/J x DBA/2J) recombinant inbred strains to produce F1 progeny with varying metastatic indices. When mammary tumors from the F1 progeny were analyzed by microRNA (miRNA) microarray, miR-290 was identified as a top candidate for a progression-associated miRNA. [Cancer Res] Abstract miR203 Mediates Subversion of Stem Cell Properties during Mammary Epithelial Differentiation via Repression of ΔNP63α and Promotes Mesenchymal-to-Epithelial Transition Data presented indicate that expression of miR203, a microRNA that targets ΔNp63α and ΔNp63β is activated during luminal epithelial differentiation and that this pattern is observed in the murine mammary hierarchy. [Cell Death Dis] Full Article The Role of Versican in Modulating Breast Cancer Cell Self-Renewal There is little literature on the potential role of breast cancer stem cells on the cellular-extracellular matrix interactions involving versican. An anti-versican shRNA was used to observe the effect of reduction of versican on breast cancer self-renewal. A versican G3 construct was exogenously expressed in breast cancer cell lines. [Mol Cancer Res] Abstract MicroRNA miR-30 Family Regulates Non-Attachment Growth of Breast Cancer Cells To explore the notion that microRNAs (miRNAs) may have a role in sustaining breast tumor-initiating cells (BT-ICs), investigators performed a comprehensive profiling of miRNA expression in a model of putative BT-ICs enriched by non-attachment growth conditions. [BMC Genomics] Abstract | Full Article Methyl Antcinate A Suppresses the Population of Cancer Stem-Like Cells in MCF7 Human Breast Cancer Cell Line The authors examined the effect of Methyl antcinate A (MAA) on cancer stem-like cells in the MCF7 human breast cancer cell line. Although MAA displayed very low cytotoxic effect towards MCF7 under normal culture conditions, it did show good inhibitory effects on the self-renewal capability which was examined by mammosphere culture including primary and secondary sphere. [Molecules] Abstract | Full Article CLINICAL RESEARCH The Exportability of the ACOSOG Z0011 Criteria for Omitting Axillary Lymph Node Dissection after Positive Sentinel Lymph Node Biopsy Findings: A Multicenter Study From a multicenter database, the authors selected 188 patients with positive sentinel lymph nodes (SLNs) and then excluded patients with positive SLNs on immunohistochemistry only. They retrospectively applied the Z0011 criteria and grouped the patients as eligible or ineligible for omitting completion axillary lymph node dissection. [Ann Surg Oncol] Abstract A Retrospective Case Series of 103 Consecutive Patients with Leptomeningeal Metastasis and Breast Cancer Approximately 2-5% of patients with breast cancer (BC) develop leptomeningeal metastasis (LM). 103 consecutive patients with BC were diagnosed with LM and initially treated with intra-cerebrospinal fluid liposomal cytarabine from 2007 to 2011 at a single institution. [J Neurooncol] Abstract |