Mammary Cell News Volume 6.22 | Jun 12 2014

MicroRNA-205 Signaling Regulates Mammary Stem Cell Fate and Tumorigenesis
Knockdown of microRNA-205 in mammary epithelial cells promoted epithelial-mesenchymal transition, disrupted epithelial cell polarity, and enhanced symmetric division to expand the stem cell population. [J Clin Invest] Full Article

CXCL12-γ in Primary Tumors Drives Breast Cancer Metastasis
To investigate the effects of chemokine C-X-C motif chemokine ligand 12 (CXCL12) isoforms on tumor growth and metastasis, researchers used a mouse xenograft model of metastatic human breast cancer combining CXCR4⁺ breast cancer cells and mammary fibroblasts secreting an isoform of CXCL12. [Oncogene] Abstract

Mammary Glands Exhibit Molecular Laterality and Undergo Left-Right Asymmetric Ductal Epithelial Growth in MMTV-cNeu Mice
Scientists showed that despite their morphologic symmetry, left versus right mammary glands in wild-type mice have baseline differences in gene expression that are L-R independently regulated during pubertal development, including genes that regulate luminal progenitor cell renewal, luminal cell differentiation, mammary tumorigenesis, tamoxifen sensitivity and chemotherapeutic resistance. [Oncogene] Abstract

A Genomic Analysis of Mouse Models of Breast Cancer Reveals Molecular Features of Mouse Models and Relationships to Human Breast Cancer
The degree to which mouse models model human breast cancer and are reflective of the human heterogeneity has yet to be demonstrated with gene expression studies on a large scale. To this end, researchers have built a database consisting of 1,172 mouse mammary tumor samples from 26 different major oncogenic mouse mammary tumor models. [Breast Cancer Res] Full Article | Press Release

Mesenchymal Precursor Cells Maintain the Differentiation and Proliferation Potentials of Breast Epithelial Cells
Investigators isolated and characterized human breast epithelial and mesenchymal precursors from reduction mammoplasty tissue and tagged them with lentiviral vectors. [Breast Cancer Res] Abstract |  Full Article

Obatoclax Analog SC-2001 Inhibits STAT3 Phosphorylation through Enhancing SHP-1 Expression and Induces Apoptosis in Human Breast Cancer Cells
Researchers examined the efficacy and drug mechanism of an obatoclax analog SC-2001, a novel STAT3 inhibitor, in human breast cancer cells. STAT3-regulated proteins, including Mcl-1, survivin, and cyclin D1, were repressed by SC-2001. [Breast Cancer Res Treat] Abstract

Phenotypic Plasticity in Normal Breast Derived Epithelial Cells
Explant culture was utilized to isolate cells from pieces of breast tissue. Selective media and trypsinization were employed to select either epithelial cells or stromal cells. The primary, non-transformed epithelial cells were characterized by immunohistochemistry, flow cytometry, and in vitro cell culture. [BMC Cell Biol]
Abstract | Full Article

Age-Related Dysfunction in Mechanotransduction Impairs Differentiation of Human Mammary Epithelial Progenitors
Multipotent progenitors from women aged <30 years were exposed to a physiologically relevant range of matrix elastic modulus (stiffness). Increased stiffness causes a differentiation bias towards myoepithelial cells while reducing production of luminal cells and progenitor maintenance. [Cell Rep]
Abstract | Full Article | Graphical Abstract | Press Release

CLINICAL RESEARCH

Final Report of the Phase I/II Clinical Trial of the E75 (nelipepimut-S) Vaccine with Booster Inoculations to Prevent Disease Recurrence in High-Risk Breast Cancer Patients
E75 is a human leukocyte antigen-A2/A3-restricted immunogenic peptide derived from the HER2 protein. Investigators conducted phase I/II clinical trials vaccinating breast cancer patients with nelipepimut-S and GM-CSF in the adjuvant setting to prevent disease recurrence. [Ann Oncol] Abstract

Significant Survival Impact of MACC1 Polymorphisms in HER2 Positive Breast Cancer Patients
Researchers aimed to investigate the influence of metastasis-associated in colon cancer-1 (MACC1) polymorphisms on event-free and overall survival in patients with human epidermal growth factor 2 (HER2)-positive breast cancer. [Eur J Cancer] Abstract

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Eisai Presents Data on Halaven^® from Pooled Analysis of Over 1,800 Advanced Breast Cancer Patients
Eisai Co., Ltd. announced that it has presented data from a pooled analysis of two randomized open-label Phase III clinical studies on the use of Halaven^®, a non-taxane microtubule dynamics inhibitor, in patients with locally advanced or metastatic breast cancer who had progressed after receiving prior therapy including an anthracycline and a taxane in either the adjuvant or metastatic setting. [Press release from Eisai Co., Ltd. discussing research presented at the 50^th Annual Meeting of the American Society of Clinical Oncology (ASCO), Chicago] Press Release

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Immune Design Announces Treatment of First Patient in Phase I Clinical Trial of LV305 Immuno-Oncology Agent
Immune Design announced treatment of the first patient in a Phase I clinical trial of LV305, an immuno-oncology investigational agent from the company’s DCVex™ lentiviral vector platform. [Immune Design Corp] Press Release

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NEW Postdoctoral Fellow – Cancer Biology (The University of Queensland)

NEW Postdoctoral Fellow – Tumor Microenvironment of Bone Metastasis of Breast and Prostate Cancer (University of Edinburgh)

Postdoctoral Scholar – Cancer and RNA Research (Northwestern University)

PhD Scholarship – Breast Cancer (Royal College of Surgeons in Ireland)

Postdoctoral Research Associates – Pattern Formation during Branching Morphogenesis of the Mammary Gland (Princeton University)

Postdoctoral Positions – Breast Cancer, Mouse Models, Tumor Microenvironment (National Cancer Institute)

Postdoctoral Position – Breast Cancer Research (University of Oklahoma Health Sciences Center)

Postdoctoral Fellow – Mechanisms that Contribute to Metastases of Breast Cancer Cells (Icahn School of Medicine at Mount Sinai)

Postdoctoral Position – Breast Cancer Metastasis (Weizmann Institute of Science)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

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