LABORATORY RESEARCH CD95 and CD95L Promote and Protect Cancer Stem Cells The authors report that stimulation of CD95 on cancer cells or reducing miR-200c levels increases the number of cancer stem cells (CSCs), which are more sensitive to induction of death induced by CD95R/L elimination than non-CSC, while becoming less sensitive to CD95-mediated apoptosis. [Nat Commun] Abstract Targeting of miR-34a-NOTCH1 Axis Reduced Breast Cancer Stemness and Chemoresistance Researchers found that primary and mature microRNA (miR)-34a were suppressed by treatment with p53 RNAi or the dominant negative p53 mutant in MCF7 cells. Ectopic miR-34a expression reduced cancer stem cell properties and increased sensitivity to doxorubicin treatment, by directly targeting NOTCH1. [Cancer Res] Abstract A Kinase Inhibitor Screen Identifies Mcl-1 and Aurora Kinase A as Novel Treatment Targets in Antiestrogen-Resistant Breast Cancer Cells Scientists performed a kinase inhibitor screen on antiestrogen responsive MCF-7 cells and a panel of MCF-7-derived tamoxifen- and fulvestrant-resistant cell lines. They identified common and distinct molecular mechanisms involved in tamoxifen- and fulvestrant-resistant cell growth. [Oncogene] Abstract Molecular Profiling and Computational Network Analysis of TAZ-Mediated Mammary Tumorigenesis Identifies Actionable Therapeutic Targets Scientists demonstrate that the oncogenic activity of TAZ is essential for propagation of the malignant phenotype. They show that constitutively active TAZ tumor-derived cells exhibit unique tumor-initiating properties, including increased self-renewal and metastatic seeding potential, acquired chemotherapy resistance and the ability to efficiently regenerate tumor formation in vivo. [Oncotarget] Full Article Vascular Endothelial Growth Factor-C Promotes Breast Cancer Progression via a Novel Anti-Oxidant Mechanism that Involves Regulation of Superoxide Dismutase 3 In vitro and in vivo assays were performed to determine whether vascular endothelial growth factor-C expression alters tumor initiating cell characteristics and the response of breast cancer cells to chemotherapy and oxidative stress. [Breast Cancer Res] Abstract | Full Article ABCB4 Is Frequently Epigenetically Silenced in Human Cancers and Inhibits Tumor Growth Researchers identified the ATP-binding cassette sub-family B member 4 (ABCB4) as a novel epigenetically silenced target gene. They investigated the epigenetic regulation of ABCB4 in 26 human lung, breast, skin, liver, head and neck cancer cells lines and in primary cancers by methylation and expression analysis. [Sci Rep] Full Article TRAIL-Mediated Apoptosis in Breast Cancer Cells Cultured as 3D Spheroids Investigators show that the breast cancer cell lines BT20 and MCF7, cultured as 3D tumor spheroids, are more resistant to TNF-alpha-related-apoptosis-inducing-ligand (TRAIL)-mediated apoptosis by downregulating the expression of death receptors that initiate TRAIL-mediated apoptosis. [PLoS One] Full Article Sclerotium rolfsii Lectin Induces Stronger Inhibition of Proliferation in Human Breast Cancer Cells than Normal Human Mammary Epithelial Cells by Induction of Cell Apoptosis The authors investigated the influence of S. rolfsii lectin on proliferation of human breast cancer cells, non-tumorigenic breast epithelial cells and normal mammary epithelial cells. [PLoS One] Full Article Berberine-Induced Apoptosis in Human Breast Cancer Cells Is Mediated by Reactive Oxygen Species Generation and Mitochondrial-Related Apoptotic Pathway Berberine-induced oxidative stress and mitochondrial-related apoptotic pathway in human breast cancer cells were investigated. Results revealed that berberine exerted an antitumor activity in breast cancer cells by reactive oxygen species generation and mitochondrial-related apoptotic pathway. [Tumor Biol] Abstract CLINICAL RESEARCH Endocrine Therapy with or without Inhibition of Epidermal Growth Factor Receptor and Human Epidermal Growth Factor Receptor 2: A Randomized, Double-Blind, Placebo-Controlled Phase III Trial of Fulvestrant with or without Lapatinib for Postmenopausal Women with Hormone Receptor-Positive Advanced Breast Cancer – CALGB 40302 (Alliance) Investigators determined whether lapatinib would improve progression-free survival among women with hormone receptor-positive metastatic breast cancer treated with fulvestrant. [J Clin Oncol] Abstract Phase I Study to Assess the Combination of Afatinib with Trastuzumab in Patients with Advanced or Metastatic HER2-Positive Breast Cancer This Phase I study utilized a 3+3 dose escalation to determine the maximum tolerated dose of oral once-daily afatinib in combination with the recommended dose of intravenous trastuzumab. [Clin Cancer Res] Abstract |