Mesenchymal Cell News Volume 11.10 | Mar 19 2019

TOP STORY

Safe and Effective Delivery of Antitumor Drug Using Mesenchymal Stem Cells Impregnated with Submicron Carriers

Scientists evaluated the capability of human MSCs (hMSCs) associated with vincristine (VCR) loaded carriers to target tumors using a 3D spheroid model in a collagen gel. Compared to free VCR, the developed hMSC-based drug delivery platform showed enhanced antitumor activity regarding tumors that expressed the CXCL12 gene, inducing directed migration of hMSCs via the CXCL12/CXCR4 pathway. [ACS Appl Mater Interfaces] Abstract

(Ranked by impact factor of the journal)

IN VITRO

A Novel Endogenous Damage Signal, Colony Stimulating Factor 2 (CSF-2), Activates Multiple Beneficial Functions of Adipose Tissues-Derived Mesenchymal Stem Cells

Researchers identified that CSF-2 was actively secreted by stem cells in response to various types of injuries as an endogenous damage signal that promoted the therapeutic effects of MSCs by enhancing their multi-lineage differentiation and migratory capacities possibly through its receptor, CD116. [Mol Ther] Abstract | Graphical Abstract

Expression and Function of Phosphoinositide 3-Kinase Delta in Mesenchymal Stromal Cells from Normal and Leukemic Bone Marrow

Scientists found that phosphoinositide 3-kinase delta (PI3Kδ) was unexpectedly expressed in primary MSCs derived from chronic lymphocytic leukemia (CLL) patients and healthy donors. PI3Kδ inhibition in MSCs using idelalisib or duvelisib significantly reduced their ability to support CLL migration and adhesion. [Br J Haematol] Abstract

METTL3 Inhibits BMSC Adipogenic Differentiation by Targeting the JAK1/STAT5/C/EBPβ Pathway via an m⁶A-YTHDF2-Dependent Manner

The authors found that the deletion of N⁶-methyladenosine (m⁶A) “writer” protein methyltransferase-like (METTL)3 in porcine bone marrow-derived MSCs (pBMSCs) could promote adipogenesis and janus kinase (JAK)1 protein expression via an m⁶A-dependent way. Knockdown of METTL3 decreased mRNA m6A levels of JAK1, leading to enhanced YTH m⁶A RNA binding protein 2 (YTHDF2)-dependent JAK1 mRNA stability. [FASEB J] Abstract

Uptake and Distribution of Carboxylated Quantum Dots in Human Mesenchymal Stem Cells: Cell Growing Density Matters

Researchers investigated the uptake dynamics, cytotoxity, subcellular and extracellular distribution of non-targeted carboxylated quantum dots in human bone marrow MSCs at different cell growing densities. [J Nanobiotechnology] Abstract

Cell Cycle-Dependent Expression of Bk Channels in Human Mesenchymal Endometrial Stem Cells

Researchers aimed to verify the role of calcium-activated potassium channels of big conductance (BK) channels as potassium transporting pathway regulating cell cycle passageway of endometrium-derived MSCs. [Sci Rep] Full Article

IN VIVO

Hypoxic BMSC-Derived Exosomal miRNAs Promote Metastasis of Lung Cancer Cells via STAT3-Induced EMT

Investigators demonstrated that exosomes derived from hypoxic bone marrow-derived MSCs (BMSCs) were taken by neighboring cancer cells and promoted cancer cell invasion and EMT. Exosome-mediated transfer of select microRNAs, including miR-193a-3p, miR-210-3p and miR-5100, from BMSCs to epithelial cancer cells activated STAT3 signaling and increased the expression of mesenchymal related molecules. [Mol Cancer] Full Article

Mesenchymal Stem Cell Exosomes Enhance Periodontal Ligament Cell Functions and Promote Periodontal Regeneration

Scientists investigated the therapeutic effects of human MSC exosome-loaded collagen sponge for the regeneration of surgically created periodontal intrabony defects in an immunocompetent rat model. They observed that relative to control rats, exosome-treated rats repaired the defects more efficiently with regeneration of periodontal tissues including newly-formed bone and periodontal ligament. [Acta Biomater] Abstract

Bone Marrow-Derived Mesenchymal Stem Cells Promote Colorectal Cancer Progression via CCR5

The authors found that the xenografts in which C-C chemokine receptor type 5 (CCR5)-overexpressing HCT116 cells were inoculated into immunocompromised mice were highly promoted in vivo by a mixture with MSCs. Notably, the CCR5 inhibitor, maraviroc, significantly abolished the MSC-induced tumor growth in vivo. [Cell Death Dis] Full Article

HMGB1-Modified Mesenchymal Stem Cells Attenuate Radiation-Induced Vascular Injury Possibly via Their High Motility and Facilitation of Endothelial Differentiation

Female F344 rats received an intravenous injection of male F344 high mobility group box 1 (HMGB1)-modified MSC (MSC-H) cells or vehicle control at four doses of 2×10⁶ cells with a 15-day interval starting from 30 days after irradiation to the abdominal aorta. The aortas were procured for histological and biomedical analysis at 90 days after irradiation. [Stem Cell Res Ther] Full Article

Calpain Inhibitor MDL28170 Improves the Transplantation-Mediated Therapeutic Effect of Bone Marrow-Derived Mesenchymal Stem Cells Following Traumatic Brain Injury

Significant neurological function improvement was observed when bone marrow-derived MSCs were transplanted into a MDL28170-preconditioned traumatic brain injury brain compared with the one without MDL28170-precondition group. [Stem Cell Res Ther] Full Article

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Production and Quality Testing of Multipotent Mesenchymal Stromal Cell Therapeutics for Clinical Use

The authors examine the current challenges and concepts as well as discuss possible future options for the production and quality testing of MSC therapeutics [Transfusion] Abstract

Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field.

Bone Therapeutics to Present Data

In the poster presentation, Bone Therapeutics will highlight the in vitro chondro-osteogenic characteristics and the potent in vivo osteogenic properties of its proprietary allogenic ex vivo differentiated bone-forming cells derived from human bone marrow MSCs. [Press release from Bone Therapeutics discussing research presented at the 2019 Herbert Fleisch/International Federation of Musculoskeletal Research Societies (IFMRS) Workshop, Bruges] Press Release

BrainStorm Announces First Patient Enrolled in Phase II Clinical Trial of Progressive MS

BrainStorm Cell Therapeutics Inc. announced that the first patient has been enrolled in its Phase II open-label, multicenter study of repeated intrathecal administration of autologous MSC-NTF cells in participants with progressive Multiple Sclerosis (MS). [BrainStorm Cell Therapeutics Inc.] Press Release

National MS Society Funds New Clinical Trial of Individuals’ Own Stem Cells to Treat Progressive MS

The National MS Society has committed $1 million over three years to help support a Phase II clinical trial of stem cells in progressive MS. The trial is being led by Dr. Saud Sadiq at the Tisch MS Research Center of New York, and follows a Phase I trial at Tisch MSRCNY that suggested the procedure was safe. [National MS Society] Press Release

Trump Proposes Slashing Science Spending at the NSF

US President Donald Trump has proposed cuts across the board at the National Science Foundation (NSF) in his 2020 budget request to Congress. The biggest decreases would hit funding for research in Antarctica and the Arctic, the geosciences, maths and physical sciences. [Nature News] Editorial

Appeals Court Dismisses Charges of Institutional Racism at UK-Kenyan Research Partnership

An appeals court in Kenya has dismissed charges of institutional racism at a UK-Kenyan research partnership. The Court of Appeal in Nairobi overturned a 2014 verdict by a lower court that found that six Kenyan academics working for the Kenya Medical Research Institute-Wellcome Trust Research Programme, had faced “systemic discrimination” in their careers. [ScienceInsider] Editorial

NEW DASCS2019 Stem Cell Conference
May 2-3, 2019
Vejle, Denmark

Visit our events page to see a complete list of events in the community.

NEW Junior Professorship – Translational Pediatric Sarcoma Research (German Research Center)

NEW Associate Professor – Department of Endocrinology (University of Southern Denmark)

NEW Assistant Professor – Medical Biotechnology (Aarhus University)

Cell Biologist – MSCs & Liver Disease (Hepatx Corporation)

Postdoctoral Fellow & Research Associate – Morphogenetic Signaling Pathways (University of Rochester Medical Center)

Faculty Position – Bioengineering (University of Pittsburgh)

Assistant Professor – Biomedical Engineering (Binghamton University)

Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California at Davis)

Research Scientist – Stem Cell Biology (MD Anderson Cancer Center)

Assistant Professor – Department of Biological Sciences (Rutgers University Newark)

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