Effects of a miR-31, Runx2 and Satb2 Regulatory Loop on the Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells The effects of miR-31, Runx2 and Satb2 on the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) were investigated using mimics and inhibitors of miR-31, small interfering RNA for knockdown of Runx2 and plasmids for over-expression of Runx2. The results showed that miR-31 expression decreased progressively in BMSC cultures during differentiation. [Stem Cells Dev] Abstract Glycosaminoglycan Mimetic Associated to Human Mesenchymal Stem Cell Based Scaffolds Inhibit Ectopic Bone Formation but Induce Angiogenesis In Vivo Researchers developed a strategy associating the glycosaminoglycan mimetic [OTR4120] with bone substitutes to optimize stem cell-based therapeutic products. They showed that [OTR4120] was able to potentiate proliferation, migration and osteogenic differentiation of human mesenchymal stem cells in vitro. [Tissue Eng Part A] Abstract Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo(poly(ethylene glycol) fumarate) Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model Researchers analyzed the morphology of bone mesenchymal stem cells (BMSCs) seeded into rehydrated freeze-dried oligo[poly(ethylene glycol) fumarate] (OPF) hydrogel and in vivo gross morphological and histological outcome of defects implanted with BMSCs-OPF scaffold in a porcine model. The analyses were based on magnified histologic sections for different types of cartilage repair tissues, the outcome of the subchondral bone, scaffold, and statistical assessment of neotissue filling percentage, cartilage phenotype and Wakitani scores. [Tissue Eng Part A] Abstract Poly(L-lactide-co-glycolide) Scaffolds Coated with Collagen and Glycosaminoglycans: Impact on Proliferation and Osteogenic Differentiation of Human Mesenchymal Stem Cells Researchers analyzed poly(L-lactide-co-glycolide) scaffolds modified with artificial extracellular matrices (aECM) consisting of collagen type I, chondroitin sulphate, and sulphated hyaluronan. They investigated the effect of these aECM coatings on proliferation and osteogenic differentiation of human mesenchymal stem cells in vitro. They found that scaffolds were homogeneously coated, and cross-linking of aECM did not significantly influence the amount of collagen immobilized. [J Biomed Mater Res A] Abstract Autologous Rabbit Adipose Tissue-Derived Mesenchymal Stromal Cells for the Treatment of Bone Injuries with Distraction Osteogenesis Although distraction osteogenesis is the most important therapy for treating bone defects, this treatment is restricted in many situations. Researchers examined the therapeutic potential of adipose tissue-derived mesenchymal stem cells (MSCs) and osteoblasts differentiated from adipose tissue-derived MSCs in the treatment of bone defects. [Cytotherapy] Abstract Inflammatory Environment Induces Gingival Tissue-Specific Mesenchymal Stem Cells to Differentiate towards a Pro-Fibrotic Phenotype Under the influence of inflammatory cytokines, gingival tissue-specific mesenchymal stem cells exhibited a higher rate of proliferation than those under normal conditions, while their potential for osteogenic and adipogenic differentiation was suppressed. [Biol Cell] Abstract S100A16 Inhibits Osteogenesis but Stimulates Adipogenesis Researchers aimed to demonstrate, in the mouse bone marrow-derived mesenchymal stem cell model, whether S100A16 significantly stimulates adipogenic, rather than osteogenic differentiation. The overexpression of S100A16 led to a significant increase in Oil Red O staining (a marker of adipocyte differentiation) but a decrease in Alizarin Red S staining (a marker of osteoblast differentiation). In contrast, reducing the expression of S100A16 resulted in minimal Oil Red O staining but increased Alizarin Red S staining. [Mol Biol Rep] Abstract Downregulated MicroRNA-32 Expression Induced by High Glucose Inhibits Cell Cycle Progression via PTEN Upregulation and Akt Inactivation in Bone Marrow-Derived Mesenchymal Stem Cells Using a microRNA microarray approach, the authors identified that miRNA-32-5p expression is significantly reduced under hyperglycemic conditions in rat bone marrow-derived mesenchymal stem cells. [Biochem Biophys Res Commun] Abstract Engineering Tubular Bone Using Mesenchymal Stem Cell Sheets and Coral Particles Rabbit bone marrow mesenchymal stem cells were continuously cultured to form a cell sheet with osteogenic potential and coral particles were integrated into the sheet. The composite sheet was then wrapped around a cylindrical mandrel to fabricate a tubular construct. The resultant tubular construct was cultured in a spinner-flask bioreactor and subsequently implanted into a subcutaneous pocket in a nude mouse for assessment of its histological characteristics, radiological density and mechanical property. [Biochem Biophys Res Commun] Abstract Comparison of Cell Proliferation; Apoptosis; Cellular Morphology and Ultrastructure between Human Umbilical Cord and Placenta-Derived Mesenchymal Stem Cells The authors compared various properties of human umbilical cord-derived mesenchymal stem cells (MSCs) with human placenta-derived MSCs, including cell proliferation, apoptosis, cellular morphology, ultrastructure, and their ability to secrete various growth factors (i.e. vascular endothelial growth factor, insulin-like growth factors-1, and hepatocyte growth factor), which will allow the selection appropriate MSC sources for cellular therapy. [Neurosci Lett] Abstract |