Adipose Tissue-Derived Mesenchymal Stem Cells Cultured at High Density Express IFN-β and Suppress the Growth of MCF-7 Human Breast Cancer Cells Scientists found that type I interferon is expressed in adipose tissue-derived stem cells (ASCs) cultured at high density, and ASCs and their conditioned medium suppress the growth of MCF-7 cells in vitro. [Cancer Lett] Abstract Identification of Stable Reference Genes for Gene Expression Analysis of Three-Dimensionally Cultivated Human Bone Marrow-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering Mesenchymal stromal cells isolated from bone marrow aspirates were cultivated on human cancellous bone cube scaffolds for 14 days. Osteogenic differentiation was assessed by cell-specific alkaline phosphatase activity and expression of osteogenic marker genes. [Tissue Eng Part C Methods] Abstract The Use of Adipose Mesenchymal Stem Cells and Human Umbilical Vascular Endothelial Cells on a Fibrin Matrix for Endothelialized Skin Substitute Scientists produced a new model of endothelialized skin substitute that promotes the formation of capillary-like structures by seeding human umbilical vein endothelial cells with dermal fibroblasts and adipose derived mesenchymal stem cells in a fibrin matrix. [Tissue Eng Part A] Abstract Immunomodulatory Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Pro-Inflammatory Cytokine-Stimulated Human Corneal Epithelial Cells Scientists investigated the modulatory effect of rat bone marrow mesenchymal stem cells on human corneal epithelial cells stimulated with pro-inflammatory cytokines interferon gamma and tumor necrosis factor alpha in an in vitro co-cultured model. [PLoS One] Full Article Toll Like Receptor 3 & 4 Responses of Human Turbinate Derived Mesenchymal Stem Cells: Stimulation by Double Stranded RNA and Lipopolysaccharide Researchers investigated the influence of Toll like receptor on the immune response, proliferation, and differentiation potential of human turbinated mesenchymal stromal cell cultures in vitro. [PLoS One] Full Article In Vitro Characterization of Scaffold-Free Three-Dimensional Mesenchymal Stem Cell Aggregates Scientists describe a novel method for generating and culturing rabbit mesenchymal stem cells (MSCs) as scaffold-free three dimensional cell aggregates by using micropatterned wells via a forced aggregation technique. The viability and proliferative capability of MSC aggregates were assessed via Live/Dead staining and 5-ethynyl-2’-deoxyuridine incorporation. [Cell Tissue Res] Abstract High Extracellular Magnesium Inhibits Mineralized Matrix Deposition and Modulates Intracellular Calcium Signaling in Human Bone Marrow-Derived Mesenchymal Stem Cells Researchers examined the effects of extracellular Mg2+ on the deposition of calcium phosphate matrix and Ca2+ signaling with or without ATP stimulation in human bone marrow-derived mesenchymal stem cells. [Biochem Biophys Res Commun] Abstract Bone Marrow Mesenchymal Stem Cells Ameliorate Colitis-Associated Tumorigenesis in Mice Mesenchymal stem cells (MSCs) were isolated from the bone marrow of allogenic mice and identified by flow cytometry. Mice in the model of colitis-associated tumorigenesis induced by azoxymethane and dextran sulfate sodium were injected with MSCs. [Biochem Biophys Res Commun] Abstract Low Frequency Magnetic Force Augments Hepatic Differentiation of Mesenchymal Stem Cells on a Biomagnetic Nanofibrous Scaffold Researchers evaluated hepatic trans-differentiation potential of human mesenchymal stem cells on a biomagnetic electrospun nanofibrous scaffold fabricated from a blend of poly-l-lactide, collagen and fibrin-rich blood clot, under the influence of a low frequency magnetic field. [J Mater Sci Mater Med] Abstract Safety of Repeated Transplantations of Neurotrophic Factors-Secreting Human Mesenchymal Stromal Stem Cells Scientists studied the toxicity and tolerability of three consecutive intramuscular injections of cryopreserved human mesenchymal stem cell-neurotrophic factor-secreting cells in C57BL/B6 mice to investigate the effect of repeated administration of these cells. [Clin Transl Med] Abstract | Full Article |