Mesenchymal Stem Cells Do Not Exert Direct Beneficial Effects on CNS Remyelination in the Absence of the Peripheral Immune System The authors determined potential regenerative functions of mesenchymal stem cells in the toxic cuprizone model of demyelination that allows studying direct effects on de- and remyelination without the influence of the peripheral immune system. [Brain Behav Immun] Abstract BMP-2 Derived Peptide and Dexamethasone Incorporated Mesoporous Silica Nanoparticles for Enhanced Osteogenic Differentiation of Bone Mesenchymal Stem Cells Bone morphogenetic protein-2 (BMP-2) peptide functionalized mesoporous silica nanoparticles (MSNs-pep) were synthesized by covalently grafting BMP-2 peptide on the surface of nanoparticles via an aminosilane linker, and dexamethasone was then loaded into the channel of MSNs to construct nanoparticulate osteogenic delivery systems. [ACS Appl Mater Interfaces] Abstract Human Bone Marrow- and Adipose-Mesenchymal Stem Cells Secrete Exosomes Enriched in Distinctive miRNA and tRNA Species Researchers characterized the full small RNAome of mesenchymal stem cell (MSC)-produced exosomes. They set up a protocol for isolating exosomes released by early passage adipose- and bone marrow-MSCs and characterized them via electron microscopy, protein analysis and small RNA-sequencing. [Stem Cell Res Ther] Abstract | Full Article EphB4 Promotes Osteogenesis of CTLA4 Modified Bone Marrow Derived Mesenchymal Stem Cells through Cross-Talk with Wnt Pathway in Xenotransplantation Cytotoxic T lymphocyte-associated antigen 4-Ig modified bone marrow derived mesenchymal stem cells (MSCs-CTLA4) were investigated for their osteogenic capacity via xenotransplantation in vivo. MSCs-CTLA4 were treated with ephrinB2-FC or FC under conditions of osteogenic induction and cultured with or without immune activation conditions established by PHA and PBMCs in vitro. [Tissue Eng Part A] Abstract A Xeno-Free Microcarrier-Based Stirred Culture System for the Scalable Expansion of Human Mesenchymal Stem/Stromal Cells Isolated from Bone Marrow and Adipose Tissue Scientists optimized a previously developed xeno-free microcarrier-based system for the scalable expansion of human mesenchymal stem/stromal cells isolated from bone marrow and adipose-derived stem/stromal cells. [Biotechnol J] Abstract Multi-Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co-Cultured Human Mesenchymal Stem Cells Investigators developed multi-layered scaffolds by assembling a gelatin layer with layers containing different amounts of gelatin and hydroxyapatite nanocrystals, and using a gelatin solution (as a glue) to stick layers together. In vitro tests demonstrated that the different layers of the scaffolds promote chondrogenic and osteogenic differentiation of human mesenchimal stem cells. [Macromol Biosci] Abstract Viability and MR Detectability of Iron Labeled Mesenchymal Stem Cells Used for Endoscopic Injection into the Porcine Urethral Sphincter The authors evaluated proliferation, migration, differentiation and adhesion effects as well as the obtained iron load of an iron labeling strategy for mesenchymal stem cells. [NMR Biomed] Abstract Suppression of Ornithine Decarboxylase Promotes Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Suppression of ornithine decarboxylase by its irreversible inhibitor, α-difluoromethylornithine, or by RNA interference through siRNA, enhanced osteogenic gene expression and alkaline phosphatase activity, and accelerated matrix mineralization of human bone marrow-derived mesenchymal stem cells. [FEBS Lett] Abstract |